FastQCFastQC Report
Sat 2 Sept 2023
SRR12381709_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12381709_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82756219
Total Bases4.1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGC1950572123.570096889032595RNA PCR Primer, Index 2 (100% over 50bp)
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCT29143463.521603615070911RNA PCR Primer, Index 2 (100% over 43bp)
GGGGCTGATTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGT10565401.2766895500626967RNA PCR Primer, Index 2 (100% over 39bp)
AGTGGGTCGGAGGTTCGAATCTGGAATTCTCGGGTGCCAAGGAACTCCAG4154350.5019985265397395RNA PCR Primer, Index 1 (100% over 29bp)
AACACGAGCTGACGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC3189780.38544293571459565RNA PCR Primer, Index 2 (100% over 34bp)
TGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGCC3111940.3760369960836418RNA PCR Primer, Index 2 (98% over 50bp)
AAAAGAGATCGCGTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT2698990.32613742297723874RNA PCR Primer, Index 1 (100% over 30bp)
AAGGTCGGCTCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATG2485480.3003375492541533RNA PCR Primer, Index 2 (100% over 38bp)
GGGAGATTCCTGGAAGCAGGGCTGGAATTCTCGGGTGCCAAGGAACTCCA2331900.28177942735638994RNA PCR Primer, Index 1 (100% over 28bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCG2329530.28149304404542697RNA PCR Primer, Index 2 (100% over 45bp)
AACCGGTACTAATGAACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1781700.21529499795054674RNA PCR Primer, Index 1 (100% over 33bp)
GAAAGGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTAT1615620.19522641555192366RNA PCR Primer, Index 2 (100% over 41bp)
AGAAAGGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTA1556820.18812120935587934RNA PCR Primer, Index 2 (100% over 40bp)
TTGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATC1550380.1873430201058364RNA PCR Primer, Index 2 (100% over 42bp)
AGACTGCGATCCGAACTGAGAACTGGAATTCTCGGGTGCCAAGGAACTCC1522090.1839245459002906RNA PCR Primer, Index 1 (100% over 27bp)
GGAAAGGCGAAAAGAACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1501700.18146068273128815RNA PCR Primer, Index 1 (100% over 32bp)
AAATGGACCAGAAGCTGTTAACGGATTGGAATTCTCGGGTGCCAAGGAAC1427630.17251029798739356RNA PCR Primer, Index 1 (100% over 24bp)
GAGGACCGAACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATG1357090.16398646728894176RNA PCR Primer, Index 2 (100% over 38bp)
GAAGAGGTCTGGAAAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC1296920.15671571462200323RNA PCR Primer, Index 2 (100% over 34bp)
GGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGCC1164930.14076646010132485RNA PCR Primer, Index 2 (100% over 50bp)
GAGAAAGGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGT1145020.1383605986155554RNA PCR Primer, Index 2 (100% over 39bp)
CCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC1136540.13733590221177214RNA PCR Primer, Index 2 (100% over 44bp)
GAAAGGGCGCACGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGAT1119530.1352804675622022RNA PCR Primer, Index 2 (100% over 37bp)
ATCTCGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTA1059170.12798675589565056RNA PCR Primer, Index 2 (100% over 40bp)
AAGGGGTCGGAGGTTCGAATCTGGAATTCTCGGGTGCCAAGGAACTCCAG1016380.12281614750910745RNA PCR Primer, Index 1 (100% over 29bp)
GGCGAAAAGAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGT955760.11549101826413795RNA PCR Primer, Index 2 (100% over 39bp)
CGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCG949560.1147418298557115RNA PCR Primer, Index 2 (100% over 45bp)
AGAGGATGACGCAGGACGCGTTGGAATTCTCGGGTGCCAAGGAACTCCAG948860.11465724406766337RNA PCR Primer, Index 1 (100% over 29bp)
GGAAAAGGATGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGT946160.11433098459947766RNA PCR Primer, Index 2 (100% over 39bp)
AAAGAGATCGCGTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC908290.10975489346607292RNA PCR Primer, Index 1 (100% over 31bp)
GAAAGGCGAAAAGAACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC888250.10733332319109457RNA PCR Primer, Index 1 (100% over 33bp)
AGAGGATGACGCAGGACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT854230.10322245389195464RNA PCR Primer, Index 1 (100% over 30bp)
GAAAGGGCGCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGT833780.10075134051254807RNA PCR Primer, Index 2 (100% over 39bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)