FastQCFastQC Report
Sat 2 Sept 2023
SRR12381711_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12381711_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79627461
Total Bases3.9 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGTATGC1972835824.77582199939792RNA PCR Primer, Index 9 (100% over 50bp)
AACACGAGCTGACGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG10726621.3471005938516614RNA PCR Primer, Index 6 (100% over 34bp)
ATCTCGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGA4873090.6119861086616839RNA PCR Primer, Index 9 (100% over 40bp)
GGGGCTGATTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAG4639110.5826017735263467RNA PCR Primer, Index 9 (100% over 39bp)
ACTGAGTCTCGGGTGGAGACAGCCTGGCCATCTGGAATTCTCGGGTGCCA4381120.5502021469703774No Hit
TTGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATC4217750.5296853556588976RNA PCR Primer, Index 9 (100% over 42bp)
AGCACCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGA3895440.4892081137686909RNA PCR Primer, Index 9 (100% over 40bp)
AAGGTCGGCTCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCA3761960.4724450525930997RNA PCR Primer, Index 9 (100% over 38bp)
ACTGAGTCTCGGGTGGAGACAGCCTGGCCTGGAATTCTCGGGTGCCAAGG3504870.4401584523710984Illumina Small RNA Adapter 2 (100% over 21bp)
AGACTGCGATCCGAACTGAGAACTGGAATTCTCGGGTGCCAAGGAACTCC2557520.32118567738835724RNA PCR Primer, Index 1 (100% over 27bp)
ACGAGCTGACGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATC2441790.30665174668824363RNA PCR Primer, Index 9 (100% over 37bp)
GGTTAAGCGACTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCA2391990.30039762287535454RNA PCR Primer, Index 9 (100% over 38bp)
ATTTAGCTACCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGA2308980.2899728273390508RNA PCR Primer, Index 9 (100% over 35bp)
ACGCAGTCGAGTTGCAGACTGCGATCCGAACTGAGAACTGGAATTCTCGG2293780.2880639381431489No Hit
ACAACACGAGCTGACGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA2284180.2868583239141582RNA PCR Primer, Index 1 (100% over 32bp)
ACGACACGAGCTGACGACAACCATGCACCTGGAATTCTCGGGTGCCAAGG2122440.26654623585197573Illumina Small RNA Adapter 2 (100% over 21bp)
AGGATGTGATGAGCCGACATCGAGGTGCCTGGAATTCTCGGGTGCCAAGG2103560.26417519453496074Illumina Small RNA Adapter 2 (100% over 21bp)
TGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGTATGCC1956380.2456916213867475RNA PCR Primer, Index 9 (98% over 50bp)
AGCTACCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAG1878820.23595126309502698RNA PCR Primer, Index 9 (100% over 39bp)
CGAACTGAGAACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCA1850910.23244619089386764RNA PCR Primer, Index 9 (100% over 38bp)
ATGCGGAACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGA1664350.20901708771048222RNA PCR Primer, Index 9 (100% over 40bp)
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCT1497810.1881021925338044RNA PCR Primer, Index 9 (100% over 43bp)
AGGATTGTCAGAGGATGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1280050.16075484310619925RNA PCR Primer, Index 1 (100% over 31bp)
ACCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCT1278940.16061544396097222RNA PCR Primer, Index 9 (100% over 43bp)
ATTTTCGGCGCAGAGTCACTCGACCTGGAATTCTCGGGTGCCAAGGAACT1208380.15175417937789074RNA PCR Primer, Index 1 (100% over 25bp)
ATTTCGGAGAGAACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGA1119240.14055954892245026RNA PCR Primer, Index 9 (100% over 35bp)
AAATTGAAGAGTTTGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1026970.1289718379944326RNA PCR Primer, Index 1 (100% over 32bp)
GAAAGGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGAT1011780.1270642046466859RNA PCR Primer, Index 9 (100% over 41bp)
ATCGAGGTGCCAAACACCGCCGTCGATATGAACTCTTGGGCGGTATCTGG894680.11235822274931007No Hit
ACGGCGTGGACTACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGA887010.11139498721427274RNA PCR Primer, Index 9 (100% over 35bp)
AGAGGATGACGCAGGACGCGTTGGAATTCTCGGGTGCCAAGGAACTCCAG867760.1089774795155154RNA PCR Primer, Index 1 (100% over 29bp)
AGTGGGTCGGAGGTTCGAATCTGGAATTCTCGGGTGCCAAGGAACTCCAG822270.10326462625751685RNA PCR Primer, Index 1 (100% over 29bp)
ACGCCCAATAATTCCGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTC817050.10260907352050318RNA PCR Primer, Index 1 (100% over 31bp)
AATAATTCCGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATC811460.10190705440174716RNA PCR Primer, Index 9 (100% over 37bp)
TTGGATGATAACGAGGCGCAAAAAATGTGGAATTCTCGGGTGCCAAGGAA808160.1014926245105316RNA PCR Primer, Index 1 (100% over 23bp)
ATGGAGTCGAGTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGA801570.10066502057625572RNA PCR Primer, Index 9 (100% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)