FastQCFastQC Report
Sat 2 Sept 2023
SRR12391503_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12391503_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20444725
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG323673415.831633832198769TruSeq Adapter, Index 1 (100% over 49bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC242170411.845128755705934No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG10103494.941856640282518No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCT7984753.905530644212627Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC7392793.6159889653688175No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACG3348381.6377720903558255No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTG3273341.6010682462102084Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACGT2459911.203200336517121No Hit
GTGAACAGGGAAGAGCCCAGCGCCGAATCCCCAGATCGGAAGAGCACACG1775670.8685223205496773No Hit
TTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTC1650120.8071128371743811Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT1601210.7831897959008985No Hit
AGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAG1467780.7179260175913347Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT1372840.6714886113655233No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCT1351970.6612805992743849Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTG1338770.6548241661357636Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC1271200.6217740761981392No Hit
TGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACGT1184180.5792105298555006No Hit
GTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGT1105430.5406920366989529Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC1051270.5142010958817006No Hit
GAGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCA971430.47514945786749396Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA827770.4048819438754984Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC670300.3278596312740817TruSeq Adapter, Index 1 (100% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC662400.32399555386536133TruSeq Adapter, Index 1 (100% over 50bp)
CTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC651680.3187521475588446Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT645720.31583697017201257TruSeq Adapter, Index 1 (100% over 35bp)
CCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTC605930.2963747372488502No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGT605110.29597365579629953No Hit
AGATCGGAAGAGCACACGTCTAAGATCGGAAGAGCACACGTCTGAACTCC597100.2920557747780907Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT580720.28404392820152874No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT550930.2694729325045947No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACACGTCTG526020.2572888605740601Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCG518020.25337587079307744TruSeq Adapter, Index 1 (100% over 50bp)
AGAGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCC488550.23896139468738267Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCCGGGGCTACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTC460010.22500180364372718No Hit
GACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACG441640.2160166008591458No Hit
TCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA433760.21216230592487795Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACGTC411050.20105430618411352No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGT403590.19740544321334721TruSeq Adapter, Index 1 (100% over 50bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCTG385110.18836643681927737Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA338310.16547544660052899TruSeq Adapter, Index 1 (100% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATATCGTATG326000.159454333575042TruSeq Adapter, Index 1 (97% over 49bp)
ACGCCGGGTTAAGGCGCCCGATGCCGACGCTAGATCGGAAGAGCACACGT315270.154206036031299No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGAAGATCGGAAGAGCACACGT306430.14988218232331324No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTCT282380.13811875679423422Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC274860.13444054640011055TruSeq Adapter, Index 1 (100% over 36bp)
GTTCTACAGTCCGACGATCATCGGAAGAGCACACGTCTGAACTCCAGTCA274020.13402968247310737Illumina Multiplexing Index Sequencing Primer (96% over 32bp)
GGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTC272150.13311502111180268TruSeq Adapter, Index 1 (100% over 50bp)
CGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGA269570.13185308190743578Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTGAACAGGGAAGAGCCCAGCGCCGAATCCCCAGATCGGAAGAGCACAC264990.12961289525782324No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT264210.12923137875417742Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG262490.12839008595126614No Hit
TGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACGTC257950.12616946425055853No Hit
CAGAGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTC253800.12413960080167377Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCT252220.12336678531992971TruSeq Adapter, Index 1 (100% over 50bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACG243730.11921412491486189No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGAAGATCGGAAGAGCACACGTC240240.11750708312290824No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATGTCGTATG232950.11394137118498782TruSeq Adapter, Index 1 (97% over 49bp)
TTCTACAGTCCGACGATCGATCGGAAGAGCACACGTCTGAACTCCAGTCA213900.10462356426902293Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)