FastQCFastQC Report
Sat 2 Sept 2023
SRR12391506_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12391506_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65732312
Total Bases4.9 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCT28752844.3742322649475645Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC22541933.4293529793992334No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT14008712.131175608124053No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG12133101.8458349677400665TruSeq Adapter, Index 2 (100% over 49bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT7804101.1872547553172934No Hit
ATAAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGT6857731.0432814229933065No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT6809851.0359973341573623Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC6551500.9966939851438665No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTG5606530.8529336378735621Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTGA5415370.8238520501150179Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG5191600.7898094319274818No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCT5030420.7652887669613689Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGACCTCAGATCAGACGTGGCGACCCAGATCGGAAGAGCACACGTCTGA4024880.6123137734756691Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAGATCGGAAGAGCACACGT3747760.5701549034210146No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC3510390.5340432875691334No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCT3422480.5206693475196795Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTC3385930.5151089162967522No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACACGTCTG3343450.508646341239298Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC3174340.48291926807625446No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT2943000.4477250092770204No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCTG2867130.4361827406892367Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTAGATCGGAAGAGCACACGTC2765370.42070176993013725No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTG2751760.41863125094398024Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACGT2741120.4170125645359926No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCT2658130.40438711481805173Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAATACAAGCTTGGGCTGCAGGTCGACAGATCGGAAGAGCACACGTCTGA2567870.3906556641427735Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA2516440.38283150606356275Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TAAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTC2491700.3790677559006292No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC2485060.378057598217449No Hit
GCGACCTCAGATCAGACGTGGCGACCAGATCGGAAGAGCACACGTCTGAA2444820.3719357992458869Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGCCGGGTTAAGGCGCCCGATGCCGACGCTAGATCGGAAGAGCACACGT2333850.3550536910979185No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGAAGATCGGAAGAGCACACGTC2274090.3459622719492964No Hit
GGGGTCGGCGGCGACGTCGGCTACCCACCCGAAGATCGGAAGAGCACACG2103420.3199978725835781No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC2078890.3162660701786969No Hit
GTCAACAAGTACCGTAAGGGAAAGTTGAGATCGGAAGAGCACACGTCTGA2073010.31537153295323006Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC2055800.31275333811474637No Hit
CTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCTG1975680.3005645077568548Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTGAACCCCATTCGTGATGGGGATCGGGAGATCGGAAGAGCACACGTCT1834750.27912451946007927Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT1791520.2725478452667236No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTAGATCGGAAGAGCACACGT1746210.2656547361364682No Hit
CGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTCT1663630.25309166061282007Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGACCTCAGATCAGACGTGGCGACCCGCAGATCGGAAGAGCACACGTCT1624290.2471067806043396Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAATACAAGCTTGGGCTGCAGGTCGACCCGAGATCGGAAGAGCACACGTC1517840.23091230991540354No Hit
TCCCGGAGTCGGGTTGCTTGGGAATGCAGCCCAAGATCGGAAGAGCACAC1487550.22630422614679976No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG1461470.22233661886105574Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTCAACAAGTACCGTAAGGGAAAGTTGAAGATCGGAAGAGCACACGTCTG1293580.1967951469590785Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA1242190.18897707416711584Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCGACCTCAGATCAGACGTGGCGACCCGAGATCGGAAGAGCACACGTCTG1239400.18855262538156273Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAAGGCTAAATACCGGCACGAGACCGAAGATCGGAAGAGCACACGTCTGA1231360.18732948264469992Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAG1200660.18265902468180337Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1200500.18263468353281107Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTAAGGCTAAATACCGGCACGAGACCGAAGATCGGAAGAGCACACGTCTG1198490.18232889784859538Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC1195940.1819409607865307Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTC1173020.17845409119338446Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGT1165690.17733896230517499Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACACG1131900.17219841590236473No Hit
ATCTAAGGCTAAATACCGGCACGAGACCGAAGATCGGAAGAGCACACGTC1125930.17129018678058974No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG1116410.1698418884155482No Hit
GAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACGTC1114770.1695923916383772No Hit
AGTCAACAAGTACCGTAAGGGAAAGTTGAAGATCGGAAGAGCACACGTCT1044700.15893248970156412Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACGT941840.14328417354314268No Hit
CTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTCT941480.14322940595791Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGCGCCGCGACCGGCTCCGGGACGGCTGGAGATCGGAAGAGCACACGTC931270.1416761363878392No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACACGTCT931220.1416685297787791Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACAGATCGGAAGAGCA910870.13857263989132163No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAAGATCGGAAGAGCACACGTCTGA909580.13837638937757127Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT902290.13726734577660982No Hit
GACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACG878610.13366485572575024No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCAGATCGGAAGAGCACACGTC870770.13247213942512778No Hit
CGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACGT831820.12654659096731605No Hit
AACGCCGGGTTAAGGCGCCCGATGCCGACGCTAGATCGGAAGAGCACACG829980.12626666775390466No Hit
CATAAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACG827690.12591828505895244No Hit
ACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGATCGGAAGAGCACACGTC780900.11880002030051826No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGAGATCGGAAGAGCACACGT771040.11729999699386809No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT762130.11594449925935969No Hit
AGATTAAGCCATGCATGTCTAAGTACGAGATCGGAAGAGCACACGTCTGA746410.11355298137086674Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCTAAGGCTAAATACCGGCACGAGACCGAGATCGGAAGAGCACACGTCT743740.1131467884470578Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTCCCTCAGTAACGGCGAGTGAACAGGGAAGATCGGAAGAGCACACGTC742980.11303116798934443No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT738610.11236635035749237Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAATACAAGCTTGGGCTGCAGGTCGACCAGATCGGAAGAGCACACGTCTG735020.11182019582697776Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAAGATCGGAAGAGCACACGT728370.11080851682198552No Hit
CGCGACCTCAGATCAGACGTGGCGACCAGATCGGAAGAGCACACGTCTGA705810.10737641481407195Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA699840.10646818569229696Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAGATCGGAAGAGCACACG687200.10454523492190568No Hit
TCTAAGGCTAAATACCGGCACGAGACCGAAGATCGGAAGAGCACACGTCT687160.10453914963465762Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCGGTGCGCCGCGACCGGCTCCGGGACGGCTAGATCGGAAGAGCACACG684040.10406449722930786No Hit
ATAAACGATGCCGACCGGCGATGCGGCGGCAGATCGGAAGAGCACACGTC679670.10339967959745582No Hit
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA673190.10241386306326788Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)