FastQCFastQC Report
Sat 2 Sept 2023
SRR12391509_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12391509_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75598536
Total Bases5.7 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT40562025.36545046322061No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCT33397364.417725761250192Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTG32978434.3623106669684715Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC30885294.085434934877575No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTGA28560933.7779739544162605Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCT20994462.777098752282716Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTCGCCGAATCCCGGGGCCGAGGGAAGATCGGAAGAGCACACGTCTGAA20924302.767818149282679Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCCGGGTTAAGGCGCCCGATGCCGACGCTAGATCGGAAGAGCACACGTC19063112.5216242282787062No Hit
CCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTC15788992.0885311853129007No Hit
ACGCCGGGTTAAGGCGCCCGATGCCGACGCTAGATCGGAAGAGCACACGT13286391.757493028700979No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAAGATCGGAAGAGCACACGTCTGA12468611.64931897622991Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGATCGGAAGAGCACACGTCTGAA12078311.5976909923229203Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTG11577271.5314145765997373Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC10427361.3793071336725355No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC8661041.1456623974834645No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT7661831.0134892030184288Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCGGGTTAAGGCGCCCGATGCCGACGCTAGATCGGAAGAGCACACGTCT7144840.9451029580784474Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTCT7063880.9343937559849043Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGAAC5924310.7836540644120409Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCGCCGCGACCGGCTCCGGGACGGCTGGAGATCGGAAGAGCACACGTCTG5621450.743592442054698Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT5221500.6906879784021215No Hit
AACGCCGGGTTAAGGCGCCCGATGCCGACGCTAGATCGGAAGAGCACACG4848340.6413272341676035No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGATCGGAAGAGCACACGTCTGAAC4421420.5848552411120765Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCT3855070.5099397692039962Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG3849180.5091606535872599TruSeq Adapter, Index 3 (100% over 49bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCT3739260.4946206894800185Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTAGATCGGAAGAGCACACGTC3414440.45165424896587947No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGAGATCGGAAGAGCACACGTCT3389310.4483301105196005Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG3322410.4394807328014923No Hit
CAGGGAAGAGCCCAGCGCCGAATCCCCAGATCGGAAGAGCACACGTCTGA3120530.41277651196843285Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCTG3017890.3991995294723697Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGAACT2649620.35048562316074483Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCCGGGTTAAGGCGCCCGATGCCGACGCAGATCGGAAGAGCACACGTCT2552760.3376732057350952Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT2050080.2711798545940096No Hit
ACGCCGGGTTAAGGCGCCCGATGCCGACGCAGATCGGAAGAGCACACGTC2028520.26832794751475086No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGAAGATCGGAAGAGCACACGTCT1973990.26111484486948267Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGCCGGGTTAAGGCGCCCGATGCCGACGCTCAGATCGGAAGAGCACACG1960560.2593383554411689No Hit
CGCCGGGTTAAGGCGCCCGATGCCGACGCTCAGATCGGAAGAGCACACGT1958160.2590208889759452No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGAAGATCGGAAGAGCACACGTC1897880.25104718959107886No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGAGATCGGAAGAGCACACGTC1805590.23883928122629253No Hit
GCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTCTG1688580.22336146826970302Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGA1685000.22288791412574446Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC1618860.21413906745495706No Hit
GAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAA1546260.20453570688194278Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCCGCGACCGGCTCCGGGACGGCTGGAGATCGGAAGAGCACACGTCTGA1507490.19940730069164303Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGTCCTAAGGCGAGCTCAGGGAGGACAAGATCGGAAGAGCACACGTCTGA1507090.19935438961410576Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA1506790.1993147063059528Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCGTCGGGCCCATACCCGGCCGTCGCCGGCAAGATCGGAAGAGCACACG1444110.1910235404558628No Hit
GGGGTCGGCGGCGACGTCGGCTACCCACCCGAAGATCGGAAGAGCACACG1323910.17512376165591353No Hit
GCGACCTCAGATCAGACGTGGCGACCAGATCGGAAGAGCACACGTCTGAA1298250.17172951603189776Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AACGCCGGGTTAAGGCGCCCGATGCCGACGCTCAGATCGGAAGAGCACAC1230570.1627769617125919No Hit
CTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTGAA1209940.16004807288860726Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCATCTAAGGCTAAATACCGGCACGAGATCGGAAGAGCACACGTCTGAA1175470.15548846078183312Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GAACGCCGGGTTAAGGCGCCCGATGCCGACGCTAGATCGGAAGAGCACAC1143430.15125028347109792No Hit
AGCACGAGCGCACGTGTTAGGACCCGAAGATCGGAAGAGCACACGTCTGA1096130.1449935485523159Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGCTAAGACTTCACCAGTCAAAGCGAAGATCGGAAGAGCACACGTCTGAA1081840.14310330030729695Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTTGAACCCCATTCGTGATGGGGATCGGGAGATCGGAAGAGCACACGTCT1066250.1410410910602819Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACGT1046600.1384418343762636No Hit
CTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCTG1021080.13506610762938584Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC1020760.13502377876735602No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACGT1014610.13421027095022053No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTG1001800.1325157936920895Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACGTC990150.13097475855881655No Hit
CTCCATCTAAGGCTAAATACCGGCACGAGAAGATCGGAAGAGCACACGTC980280.12966917772058445No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACACG972830.1286837089014528No Hit
CCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTGAA966620.1278622644226867Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AACGCCGGGTTAAGGCGCCCGATGCCGACGCAGATCGGAAGAGCACACGT959340.12689928281150842No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTAGATCGGAAGAGCACACGTC957750.1266889612782978No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACACGT948120.12541512708658803No Hit
GAACGCCGGGTTAAGGCGCCCGATGCCGACGCAGATCGGAAGAGCACACG913300.12080921778696878No Hit
CCGCCTGGGAATACCGGGTGCTGTAGGCTAGATCGGAAGAGCACACGTCT903900.11956580746484297Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCT872890.11546387617876622Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTCTAGCATCACCAGTATTAGAGGCAGATCGGAAGAGCACACGTCTGAA818490.10826796963369766Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGAACCCCATTCGTGATGGGGATCGGGGAGATCGGAAGAGCACACGTCT813000.10754176509449864Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT803400.10627189923360422No Hit
ACAGGGAAGAGCCCAGCGCCGAATCCCCAGATCGGAAGAGCACACGTCTG803120.10623486147932812Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)