FastQCFastQC Report
Sat 2 Sept 2023
SRR12391512_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12391512_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32804595
Total Bases2.4 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCT32432619.886605824580368Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG17145795.2266427919625285TruSeq Adapter, Index 4 (100% over 49bp)
CGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTG12947243.946776358616834Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTG12188413.715458154566456Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC10897623.3219797409478766No Hit
GAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGAAC9832922.99742155024319Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTGA6042241.8418883086348117Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTCGCCGAATCCCGGGGCCGAGGGAAGATCGGAAGAGCACACGTCTGAA5796591.7670055063932353Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCGCCGCGACCGGCTCCGGGACGGCTGGAGATCGGAAGAGCACACGTCTG5106191.5565471849294283Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCT5032971.534227141045332Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGAACT4961331.5123887370046787Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CACTCGCCGAATCCCGGGGCCGAGGGAAGATCGGAAGAGCACACGTCTGA4252611.2963458320396883Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT3866591.1786732925677028No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT3347891.0205551996602915No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC2881090.8782580611039398No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGATCGGAAGAGCACACGTCTGAAC2821200.8600014723547112Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAA2782060.8480702169924671Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGCGCCGCGACCGGCTCCGGGACGGCTGGAGATCGGAAGAGCACACGTCT2248290.6853582554517134Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGATCGGAAGAGCACACGTCTGAA2198040.6700402794181729Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCCGCGACCGGCTCCGGGACGGCTGGAGATCGGAAGAGCACACGTCTGA1816630.5537730308818017Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCCGCGACCGGCTCCGGGACGGCTGGGAAGATCGGAAGAGCACACGTCT1739870.5303738698801189Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGCCGCGACCGGCTCCGGGACGGCTGGGAAGATCGGAAGAGCACACGTC1442140.4396152429255718No Hit
CCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTC1416120.4316834272759655No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCT1227420.3741609978724017Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCGGGTTAAGGCGCCCGATGCCGACGCTAGATCGGAAGAGCACACGTC1184360.3610347879618694No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGAGATCGGAAGAGCACACGTC1024910.31242879236887394No Hit
AAACCCTTGTGTCGAGGGCTGACTTTCAGATCGGAAGAGCACACGTCTGA950090.2896210119344561Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAGGGCGCACCACCGGCCCGTCTCAGATCGGAAGAGCACACGTCTGAACT854480.26047570469929593Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CAGGGAAGAGCCCAGCGCCGAATCCCCAGATCGGAAGAGCACACGTCTGA845390.2577047514227809Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGCCGGGTTAAGGCGCCCGATGCCGACGCTAGATCGGAAGAGCACACGT795870.24260930519032473No Hit
CGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTCT760600.23185776260917107Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACACGT687320.2095194286044379No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAAGATCGGAAGAGCACACGT670970.20453537073083816No Hit
GCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTGA670360.20434942117102803Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTGAA644440.19644808905581673Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACACG629660.19194262267222018No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT623760.19014409414290895Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC616410.18790355436486872No Hit
GGGGTCGGCGGCGACGTCGGCTACCCACCCGAAGATCGGAAGAGCACACG612160.18660800415307674No Hit
ACTCGCCGAATCCCGGGGCCGAGGAGATCGGAAGAGCACACGTCTGAACT604440.1842546752977746Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTGAACTC596490.18183123431336373Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCT594710.18128862740113086Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCTG589800.17979188586233116Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCT579960.17679230607785282Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAAC554190.16893669926423416Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GAATACAAGCTTGGGCTGCAGGTCGACAGATCGGAAGAGCACACGTCTGA542980.16551949505854288Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGGCGCACCACCGGCCCGTCTCAGATCGGAAGAGCACACGTCTGAACTC531180.16192243799992045Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCGCCGAATCCCGGGGCCGAGGGAGATCGGAAGAGCACACGTCTGAACT522490.15927341886098578Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGCGTCGGGCCCATACCCGGCCGTCGCCGGCAAGATCGGAAGAGCACACG482490.14708000510294364No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACG482420.14705866662886707No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGAGATCGGAAGAGCA478870.14597650115784083No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCAGATCGGAAGAGCACACGTCTG477470.14554973167630936Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC417220.1271834022032584No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACAC416890.12708280653975457No Hit
TCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCG412780.12582993327611575TruSeq Adapter, Index 4 (100% over 50bp)
CGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTGAAC408840.1246288820209486Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCCGGGTTAAGGCGCCCGATGCCGACGCTAGATCGGAAGAGCACACGTCT402730.12276633806940765Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG367050.11188981299723408No Hit
TGTCCTAAGGCGAGCTCAGGGAGGACAAGATCGGAAGAGCACACGTCTGA360360.10985046454620154Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTG357880.10909447289320293TruSeq Adapter, Index 4 (100% over 35bp)
CTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTGAA351530.10715876845911373Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCGCGACCGGCTCCGGGACGGCTGGAGATCGGAAGAGCACACGTCTGAA345160.10521696731814552Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATAAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGT332040.1012175276055077No Hit
AGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGATCGGAAGAGCACACGT331640.10109559346792728No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)