FastQCFastQC Report
Sat 2 Sept 2023
SRR12391515_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12391515_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60763644
Total Bases4.6 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCT712848011.73148865133895Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTGA20047463.2992524279814424Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGATCGGAAGAGCACACGTCTGAA15878592.613172771534242Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTG10066381.6566452137070646Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC8885931.4623760879120418No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG5430760.8937515334004655No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAAGATCGGAAGAGCACACGTCTGAA5362180.8824651793430954Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CACTCGCCGAATCCCGGGGCCGAGGGAAGATCGGAAGAGCACACGTCTGA4615810.7596335071675425Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGCTAAGACTTCACCAGTCAAAGCGAAGATCGGAAGAGCACACGTCTGAA4332080.7129394675539867Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCTCTAGCATCACCAGTATTAGAGGCAGATCGGAAGAGCACACGTCTGAA3623000.5962446886825945Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTG3411910.5615051658192192Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT3361460.5532025037866393Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC3177830.522982130564783No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCT3061980.5039164537268371Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT2964780.48792004640143044No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGAGATCGGAAGAGCACACGTCTG2927160.4817288443069675Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAA2758210.4539243893931048Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCGACCTCAGATCAGACGTGGCGACCAGATCGGAAGAGCACACGTCTGAA2611690.4298112864988808Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATGCTAAGACTTCACCAGTCAAAGCGAAGATCGGAAGAGCACACGTCTGA2044100.336401812899832Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCTG1784650.2937035836757914Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAATACAAGCTTGGGCTGCAGGTCGACCCGAGATCGGAAGAGCACACGTC1755300.2888733927807226No Hit
TGCTAAGACTTCACCAGTCAAAGCGAGATCGGAAGAGCACACGTCTGAAC1675040.2756648366908344Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT1631610.26851747074286725No Hit
ATGCTAAGACTTCACCAGTCAAAGCGAGATCGGAAGAGCACACGTCTGAA1604270.2640180697523671Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGA1536620.2528847677403942Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTTTGCAAGGAGAGCCAAAGCTAAGACAGATCGGAAGAGCACACGTCTG1474520.24266484083805112Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCTAGCATCACCAGTATTAGAGGAGATCGGAAGAGCACACGTCTGAACT1447870.2382789945909103Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAA1431560.23559482377324176Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG1272300.20938507242916504TruSeq Adapter, Index 1 (100% over 49bp)
TTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACGT1258530.20711891472473243No Hit
GCGACCTCAGATCAGACGTGGCGACAGATCGGAAGAGCACACGTCTGAAC1258220.20706789737626663Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCAACAAGTACCGTAAGGGAAAGTTGAGATCGGAAGAGCACACGTCTGAA1251260.20592247561716345Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGATCGGAAGAGCACACGTCTGAAC1170670.19265961073697294Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT1123750.1849378881885359No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC1069470.17600491504426563No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC1068480.1758419886733587No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGAAGATCGGAAGAGCACACGTC1035800.17046377271251212No Hit
CCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTGAA1021150.16805279156727337Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GAATACAAGCTTGGGCTGCAGGTCGACAGATCGGAAGAGCACACGTCTGA1009370.16611413232557282Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACTTTGCAAGGAGAGCCAAAGCTAAGACCAGATCGGAAGAGCACACGTC978070.16096302585144498No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGAGATCGGAAGAGCACACGTCT968000.1593057848867655Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCT855180.14073876148704972Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC845480.13914241219634557No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCT757970.12474070844072487Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACTTTGCAAGGAGAGCCAAAGCTAAGACCCAGATCGGAAGAGCACACGT748390.1231641078010397No Hit
ATGCTAAGACTTCACCAGTCAAAGCGAAAGATCGGAAGAGCACACGTCTG737220.12132583753535255Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGCGCCGCGACCGGCTCCGGGACGGCTGGAGATCGGAAGAGCACACGTC724960.11930818368957596No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACACGT706150.11621258264234448No Hit
ACCTCTAGCATCACCAGTATTAGAGGCAGATCGGAAGAGCACACGTCTGA678330.11163418704776824Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAACT669150.11012341524481316Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTCAACAAGTACCGTAAGGGAAAGTTGAGATCGGAAGAGCACACGTCTGA642900.10580339783440242Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)