FastQCFastQC Report
Sat 2 Sept 2023
SRR12391518_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12391518_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65094927
Total Bases4.9 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCT56455248.672755712591858Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTGA20803843.195923393538793Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC11253601.728798313269481No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGATCGGAAGAGCACACGTCTGAA10182311.5642248127876388Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG10111201.5533007664329972TruSeq Adapter, Index 2 (100% over 49bp)
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG7021261.0786186149344634No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT5987120.9197521644044551No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTG5557390.8537362673438439Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC3553840.5459473055404148No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT3537100.5433756765715398Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAA2923230.4490718608533043Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTG2898750.4453111991353796Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACTTTGCAAGGAGAGCCAAAGCTAAGACCAGATCGGAAGAGCACACGTC2876720.4419269108328519No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAAGATCGGAAGAGCACACGTCTGA2675300.410984407433163Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTCGCCGAATCCCGGGGCCGAGGGAAGATCGGAAGAGCACACGTCTGAA2524910.38788122459988317Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTC2514670.3863081373453265Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCTG2506540.38505919209341766Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACGT2351840.3612938992926438No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCT2319600.3563411323896254Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGACCTCAGATCAGACGTGGCGACCAGATCGGAAGAGCACACGTCTGAA2185740.33577731794675797Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAG2055560.3157788317359969Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCGCCGCGACCGGCTCCGGGACGGCTGGGAAGATCGGAAGAGCACACGTC1970390.302694862842384No Hit
GTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGT1854220.2848486180804842Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCT1788750.27479099869026663Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT1765100.27115784306817026No Hit
AACTTTGCAAGGAGAGCCAAAGCTAAGACCCAGATCGGAAGAGCACACGT1618010.24856161218215975No Hit
CTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1566050.24057942333970203Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC1561730.23991577715418594No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCT1552330.23847173221347956Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGCTAAGACTTCACCAGTCAAAGCGAAGATCGGAAGAGCACACGTCTGAA1531750.2353101955241458Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCGCCGCGACCGGCTCCGGGACGGCTGGAGATCGGAAGAGCACACGTCTG1501080.2305986148505858Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTTTGCAAGGAGAGCCAAAGCTAAGACAGATCGGAAGAGCACACGTCTG1464000.22490231842490585Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAATACAAGCTTGGGCTGCAGGTCGACCCGAGATCGGAAGAGCACACGTC1431780.21995262395793144No Hit
CCTCTAGCATCACCAGTATTAGAGGCAGATCGGAAGAGCACACGTCTGAA1148430.1764238863037668Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGCGCCGCGACCGGCTCCGGGACGGCTGGAGATCGGAAGAGCACACGTCT1123210.17254954445221207Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTC1106240.16994258246883048No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC1061060.16300194944530777No Hit
ACGCCGGGTTAAGGCGCCCGATGCCGACGCTAGATCGGAAGAGCACACGT1033850.15882190020736947No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC977130.1501084715864264No Hit
TACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC960980.1476274794808511Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCA960150.14749997338502277Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC906310.13922897555442376No Hit
TGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACGT867260.13323004417840426No Hit
TCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA853110.13105629567723456Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACGTC832620.1279085849500991No Hit
AACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAA795290.12217388307386842Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGACCGGCGCAAGACGGACCAGAGCGAGATCGGAAGAGCACACGTCTGAA790270.12140270162681033Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTAACCAGTGAAATTGACCTGCCCGTGAGATCGGAAGAGCACACGTCTGA766680.11777876331284617Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGCTAAGACTTCACCAGTCAAAGCGAGATCGGAAGAGCACACGTCTGAAC753040.11568336193079994Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT743090.11415482499888202No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGATCGGAAGAGCACACGTCTGAAC712470.10945092541543215Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATGCTAAGACTTCACCAGTCAAAGCGAAGATCGGAAGAGCACACGTCTGA708570.1088518003868412Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATGCTAAGACTTCACCAGTCAAAGCGAGATCGGAAGAGCACACGTCTGAA708510.10884258307870905Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCCGGGTTAAGGCGCCCGATGCCGACGCTAGATCGGAAGAGCACACGTC684680.1051817755322162No Hit
CGGGACATGTGGCGTTCCCGGAGTCGGGTTAGATCGGAAGAGCACACGTC679060.10431842100383645No Hit
CCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTGAA668890.10275608727543391Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGAGAGCGACGCTCAGACAGGCGTAAGATCGGAAGAGCACACGTCTGAA659610.1013304769509919Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTCGGGGGCTCGCACGAAAGCCGCCGTGGCGAGATCGGAAGAGCACACGT656160.10080048173339222No Hit
GAATACAAGCTTGGGCTGCAGGTCGACAGATCGGAAGAGCACACGTCTGA651640.10010611118743554Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)