FastQCFastQC Report
Sat 2 Sept 2023
SRR12391521_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12391521_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84972216
Total Bases6.4 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCT61424487.228772284813662Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTGA26916733.1677095487306106Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC24702312.907104364560764No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG10586741.245906073580569No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGATCGGAAGAGCACACGTCTGAA7219960.8496847957925447Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC7141850.8404923792972517No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT6999600.8237516131155153Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACTTTGCAAGGAGAGCCAAAGCTAAGACCAGATCGGAAGAGCACACGTC5945710.6997240133174826No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC5928310.6976762851518431No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTG5068680.5965102757823805Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCTG4683870.5512237082295229Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTG4471550.5262367171876511Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGCCGCGACCGGCTCCGGGACGGCTGGGAAGATCGGAAGAGCACACGTC4288340.5046755518297887No Hit
CTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAA4216640.49623749956103297Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG3979240.46829895550799805TruSeq Adapter, Index 3 (100% over 49bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT3771200.44381565852066285No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT3721250.4379372664589564No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACGT3614970.4254296486748092No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCT2773530.3264043390371272Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACTTTGCAAGGAGAGCCAAAGCTAAGACCCAGATCGGAAGAGCACACGT2547410.29979328772595504No Hit
ACTTTGCAAGGAGAGCCAAAGCTAAGACAGATCGGAAGAGCACACGTCTG2233560.26285768515205016Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CACTCGCCGAATCCCGGGGCCGAGGGAAGATCGGAAGAGCACACGTCTGA2192230.2579937423310226Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCCGCGACCGGCTCCGGGACGGCTGGGAAGAGATCGGAAGAGCACACGT2118980.24937327749578755No Hit
GGACCGGCGCAAGACGGACCAGAGCGAGATCGGAAGAGCACACGTCTGAA1981160.23315385819760193Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCT1905070.22419916646636592Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCT1834100.21584702463214564Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCGCGACCGGCTCCGGGACGGCTGGGAAGATCGGAAGAGCACACGTCT1582760.18626794433606392Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTCGCCGAATCCCGGGGCCGAGGGAAGATCGGAAGAGCACACGTCTGAA1541720.18143813031779704Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC1499790.176503576180713No Hit
GAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACGTC1434600.16883165669117067No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGAGATCGGAAGAGCACACGTC1329830.1565017440524324No Hit
ACGCCGGGTTAAGGCGCCCGATGCCGACGCTAGATCGGAAGAGCACACGT1319630.15530135167947132No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGAGATCGGAAGAGCACACGTCT1256490.14787068751978882Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC1214490.1429278953958315No Hit
TAACTTTGCAAGGAGAGCCAAAGCTAAGACCAGATCGGAAGAGCACACGT1196550.14081661704574117No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC1192750.1403694120440498No Hit
GGGAGAGCGACGCTCAGACAGGCGTAAGATCGGAAGAGCACACGTCTGAA1158040.13628454741017934Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AACTTTGCAAGGAGAGCCAAAGCTAAGACAGATCGGAAGAGCACACGTCT1150180.13535953916983876Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACGT1147540.13504884937919No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGAGATCGGAAGAGCACACGTCTG1131140.13311880674031143Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT1129630.1329411015949025No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT1042900.12273423585893065No Hit
GGGAGAGCGACGCTCAGACAGGAGATCGGAAGAGCACACGTCTGAACTCC1004250.11818569024962229Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGCTAAGACTTCACCAGTCAAAGCGAAGATCGGAAGAGCACACGTCTGAA903350.10631122059944864Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGGACATGTGGCGTTCCCGGAGTCGGGTTAGATCGGAAGAGCACACGTC850360.10007506453638916No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)