FastQCFastQC Report
Sat 2 Sept 2023
SRR12391524_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12391524_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98887477
Total Bases7.5 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCT56301785.693519716353973Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC36661173.7073622578114716No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTGA29994863.0332313969341134Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG22302102.2553007394455014TruSeq Adapter, Index 4 (100% over 49bp)
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG9824710.9935241850694603No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT9553730.9661213219141994Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGATCGGAAGAGCACACGTCTGAA8231690.8324299749299904Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCTG6198530.6268265899836841Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTG6012300.6079940739108957Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAA5624970.5688253124306124Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC5529520.5591729274274032No Hit
AACTTTGCAAGGAGAGCCAAAGCTAAGACCAGATCGGAAGAGCACACGTC5491590.5553372546859497No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACGT4236830.4284496003472715No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGAAGATCGGAAGAGCACACGTC4043320.4088808939882246No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTG3609450.3650057731779323Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT3358440.33962237705791604No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCT3152810.3188280352222962Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGACCGGCGCAAGACGGACCAGAGCGAGATCGGAAGAGCACACGTCTGAA2946890.29800436712527306Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACTCGCCGAATCCCGGGGCCGAGGGAAGATCGGAAGAGCACACGTCTGAA2926920.2959849000900286Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAG2838130.28700600784869856Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CACTCGCCGAATCCCGGGGCCGAGGGAAGATCGGAAGAGCACACGTCTGA2372700.239939380797429Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT2317630.23437042488201007No Hit
ACTTTGCAAGGAGAGCCAAAGCTAAGACAGATCGGAAGAGCACACGTCTG2255510.22808853743937668Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCT2052290.2075379069485209Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGCCGCGACCGGCTCCGGGACGGCTGGAGATCGGAAGAGCACACGTCTG1972580.19947723006422746Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGT1970460.1992628449808665Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1950680.197262591703093Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGCCGGGTTAAGGCGCCCGATGCCGACGCTAGATCGGAAGAGCACACGT1947370.1969278678229398No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCT1886270.19074912792041404Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAACCCTTGTGTCGAGGGCTGACTTTCAGATCGGAAGAGCACACGTCTGA1796740.18169540314998633Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC1708740.17279639968972008No Hit
TTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTC1662130.16808296160695857Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGGGACATGTGGCGTTCCCGGAGTCGGGTTAGATCGGAAGAGCACACGTC1627490.16457999024487197No Hit
GGGAGAGCGACGCTCAGACAGGCGTAAGATCGGAAGAGCACACGTCTGAA1586020.1603863348642215Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GAGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCA1459370.14757884863419055Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACGTC1405560.14213731026831639No Hit
AACTTTGCAAGGAGAGCCAAAGCTAAGACCCAGATCGGAAGAGCACACGT1391730.14073875097450408No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGAGATCGGAAGAGCACACGTCT1379720.13952423925225638Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAACT1370040.1385453488716271Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GAATACAAGCTTGGGCTGCAGGTCGACCCGAGATCGGAAGAGCACACGTC1292790.1307334395840638No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC1281750.12961701914995769No Hit
TGCTAAGACTTCACCAGTCAAAGCGAAGATCGGAAGAGCACACGTCTGAA1280410.12948151159726726Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGAGAGCGACGCTCAGACAGGAGATCGGAAGAGCACACGTCTGAACTCC1223850.12376187937325976Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC1200230.12137330594449285No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATATCGTATG1175280.11885023621342873TruSeq Adapter, Index 4 (97% over 49bp)
TGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACGT1159050.11720897682524552No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGAAGAGATCGGAAGAGCACACGT1155310.1168307691781842No Hit
GCACCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCT1150080.11630188522253429Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT1123600.11362409418130873Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AACTTTGCAAGGAGAGCCAAAGCTAAGACAGATCGGAAGAGCACACGTCT1058130.10700343785694927Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT1050540.10623589880850132No Hit
TAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAAC1032920.10445407561566163Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)