FastQCFastQC Report
Sat 2 Sept 2023
SRR12391527_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12391527_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124222030
Total Bases9.4 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC68134075.4848620651264515No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC26078502.09934582456912No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCT25637972.0638827106592927Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG19697941.5857042426371555TruSeq Adapter, Index 1 (100% over 49bp)
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG18664231.5024895342637694No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT16955391.3649261729179598Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT15191051.2228950050164211No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTGA12546481.0100044251410156Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC12204280.9824569764316362No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTG11405320.9181398822736997Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT11105470.8940016517199082No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCT8792220.7077826694669215Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAGATCGGAAGAGCACACGT8114280.6532078086310456No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCTG7426600.597848867869894Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCT7292320.5870391910355998Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACGT6740500.5426171187187973No Hit
ATAAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGT6332090.5097396975399613No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT6105510.49149977664992267No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACACGTCTG5804610.46727702002615795Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT5736130.46176431024352127No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTG5282980.42528527347363426Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACGTC4496380.3619631719108116No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC4428110.3564673673421695No Hit
CCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTC4281680.34467960312675616No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC4281190.34464015762743533No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC4052340.32621749942421646No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCT3970340.3196164158644002Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA3872960.31177722663202334Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACGT3758180.30253731966866104No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGAGATCGGAAGAGCACACGTC3350430.26971302916237966No Hit
GTCAACAAGTACCGTAAGGGAAAGTTGAGATCGGAAGAGCACACGTCTGA3338400.2687446019035432Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC3269050.26316185623435717No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG3031370.2440283740331727No Hit
CTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGTCTGAACTCCA3008840.2422146860746037Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTCT2968590.23897452005896216Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGCCGCGACCGGCTCCGGGACGGCTGGGAAGATCGGAAGAGCACACGTC2963280.23854705964795456No Hit
GAATACAAGCTTGGGCTGCAGGTCGACAGATCGGAAGAGCACACGTCTGA2910070.234263600425786Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC2905410.23388846567714278Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGT2729390.21971867630886407Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAG2628290.21158002328572476Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG2619260.21085309908395475Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTC2503250.20151417586719522Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TAAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTC2388040.1922396534656534No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACACGTCT2369030.19070932909404234Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGCGCCGCGACCGGCTCCGGGACGGCTGGAGATCGGAAGAGCACACGTC2317290.18654420636983635No Hit
CTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCTG2200300.1771263921544351Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGTCTGAAC2055060.16543442415165813Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA2039130.1641520429186353Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTCAACAAGTACCGTAAGGGAAAGTTGAAGATCGGAAGAGCACACGTCTG1995430.16063414838736736Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA1963810.158088706165887Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGCCGGGTTAAGGCGCCCGATGCCGACGCTAGATCGGAAGAGCACACGT1902370.15314272355716615No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT1885720.15180238159044737No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGAGATCGGAAGAGCACACGT1836040.147803090965427No Hit
CGGGACATGTGGCGTTCCCGGAGTCGGGTTAGATCGGAAGAGCACACGTC1733040.1395114860061456No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGAGATCGGAAGAGCACACGTCT1725880.13893509870994702Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGATCGGAAGAGCACACGTC1704500.13721398692325348No Hit
GGGGTCGGCGGCGACGTCGGCTACCCACCCGAAGATCGGAAGAGCACACG1639720.13199913091099863No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAAGATCGGAAGAGCACACGTCTGA1622370.13060243823096435Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTAGATCGGAAGAGCACACGTC1610330.12963320596193767No Hit
TCAACAAGTACCGTAAGGGAAAGTTGAAGATCGGAAGAGCACACGTCTGA1483430.11941762664802692Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACG1461780.11767477958619739No Hit
TCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1436330.11562602865208368Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA1423550.11459722562898063Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAGATCGGAAGAGCACACGTC1421230.11441046326484923No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA1408910.11341869071049636Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGCGCCGCGACCGGCTCCGGGACGGCTGGAGATCGGAAGAGCACACGTCT1401540.11282539820030313Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACGTCT1392660.11211054915138643Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT1349800.1086602754760971Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACAGATCGGAAGAGCA1323240.10652216841086883No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)