FastQCFastQC Report
Sat 2 Sept 2023
SRR12391530_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12391530_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35222163
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC25144857.138928407094136No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC13279553.770225582114307No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG11570053.284877762901728No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCT7015081.9916664402467275Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG6333511.7981604366546142TruSeq Adapter, Index 2 (100% over 49bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT4808911.3653079738458993Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTGA4687191.3307501870342262Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT4038471.1465706975463148No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCT3039190.8628629650030294Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACGT2730070.7751000414142653No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTG2028400.5758874036214073Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATAAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGT1948260.5531346839772446No Hit
GAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACGTC1827780.5189289482306921No Hit
TTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTC1579080.4483199967020765Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCTG1478870.419869160221648Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC1391100.39495019087839667No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTC1362540.38684165989465213No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT1228110.3486753496654933No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAGATCGGAAGAGCACACGT1203800.34177344531623455No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC1117610.3173030571688627No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC1018360.2891247763517533No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACGT988140.2805449512001861No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCT921880.2617329321881794Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC814910.2313628495785452No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT810990.23024991395332534No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC809650.22986947167327573No Hit
CGGGACATGTGGCGTTCCCGGAGTCGGGTTAGATCGGAAGAGCACACGTC769630.2185073074586589No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA764540.21706219461876888Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCGCCGCGACCGGCTCCGGGACGGCTGGGAAGATCGGAAGAGCACACGTC727900.2066596534687549No Hit
AGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAG698870.19841768377484367Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT695710.19752052138308487No Hit
AGATCGCAGCGAGGGAGCTGCTCTGCTACGTAGATCGGAAGAGCACACGT668790.1898776063241772No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA663000.18823375497978362Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGTCTGAACTCCA634580.1801649716969398Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GAATACAAGCTTGGGCTGCAGGTCGACCCGAGATCGGAAGAGCACACGTC612190.17380817867431933No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG582370.16534191838246845Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGATCGGAAGAGCACACGTC526780.14955924200339427No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG506930.1439235858399724No Hit
CTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCTG500650.14214061754242635Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGT498870.14163525391668877Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT478380.1358178939777208Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GAGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCA477410.13554249919290873Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCT475400.13497183577283428Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGACCTCAGATCAGACGTGGCGACCAGATCGGAAGAGCACACGTCTGAA424810.120608720140214Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCCGGGGCTACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACACGTCTG413860.11749988210548001Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCTACAGTCCGACGATCATCGGAAGAGCACACGTCTGAACTCCAGTCAC396640.11261091489469287Illumina Multiplexing Index Sequencing Primer (96% over 33bp)
AATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCTGAACTCCA395310.11223331173613614Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGGACATGTGGCGTTCCCGGAGTCGGGTTGAGATCGGAAGAGCACACGT382070.10847431487952627No Hit
GAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACGTCT381550.10832668056189507Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCGGAGTCGGGTTGCTTGGGAATGCAGCCCAAGATCGGAAGAGCACAC381030.10817904624426386No Hit
GCGACCTCAGATCAGACGTGGCGACCCAGATCGGAAGAGCACACGTCTGA378440.10744371377760077Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACAGATCGGAAGAGCA377260.10710869744143767No Hit
CTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGTCTGAAC357810.10158660613773209Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)