FastQCFastQC Report
Sat 2 Sept 2023
SRR12391536_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12391536_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58921249
Total Bases4.4 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCT40913346.9437326421916135Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTG33578405.698860864269866Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT18549483.148181736609148No Hit
GAACGCCGGGTTAAGGCGCCCGATGCCGACGCTAGATCGGAAGAGCACAC11401651.9350659046620007No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAAGATCGGAAGAGCACACGTCTGA10377401.7612321829769768Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTGA10115821.7168373331665119Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGCCGGGTTAAGGCGCCCGATGCCGACGCTAGATCGGAAGAGCACACGT9333441.584053318353791No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC9068371.5390661525182536No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCT7122191.2087642609205382Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTC6936131.1771865189076354No Hit
AGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGATCGGAAGAGCACACGT6690001.135413813105014No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCT6686251.1347773703846638Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG5168880.8772522795638633TruSeq Adapter, Index 4 (100% over 49bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC5034130.8543827711459409No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAGATCGGAAGAGCACACGT4377180.7428864924434986No Hit
CGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTCT4373910.7423315143913531Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT4114880.6983694456307264No Hit
GGGGTCGGCGGCGACGTCGGCTACCCACCCGAAGATCGGAAGAGCACACG4068180.6904436122866302No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTG3853640.6540322999602401Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTCGCCGAATCCCGGGGCCGAGGGAAGATCGGAAGAGCACACGTCTGAA3467200.5884464533329903Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAAGATCGGAAGAGCACAC2928800.4970702504965569No Hit
GACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACG2914850.49470268357685354No Hit
AGCGTCGGGCCCATACCCGGCCGTCGCCGGCAAGATCGGAAGAGCACACG2911480.49413073371883204No Hit
GGGTCGGCGGCGACGTCGGCTACCCACCCGAAGATCGGAAGAGCACACGT2760060.46843202526137895No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT2591050.4397479761503359Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAATACAAGCTTGGGCTGCAGGTCGACCAGATCGGAAGAGCACACGTCTG2577370.43742623310649775Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACGCCGGGTTAAGGCGCCCGATGCCGACGCTAGATCGGAAGAGCACACG2477020.4203950259099226No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACACGTCTG2232260.3788548338478025Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAATACAAGCTTGGGCTGCAGGTCGACAGATCGGAAGAGCACACGTCTGA2104860.3572327531617668Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAACGCCGGGTTAAGGCGCCCGATGCCGACGCAGATCGGAAGAGCACACG2077760.3526333937693683No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGAGATCGGAAGAGCACACGTCT2074130.3520173172160692Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG1838610.31204532001689234No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGA1795030.30464900701612757Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGTCCTAAGGCGAGCTCAGGGAGGACAAGATCGGAAGAGCACACGTCTGA1717540.2914975546428081Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTG1671680.28371428446807023Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGAACGCCGGGTTAAGGCGCCCGATGCCGACGCAGATCGGAAGAGCACAC1657180.28125337261604894No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGATCGGAAGAGCACACGTCTGAAC1628740.2764265910249119Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCAGATCGGAAGAGCACA1619310.27482614973080427No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTAGATCGGAAGAGCACACGT1595730.27082419790524126No Hit
GGTCGGCGGCGACGTCGGCTACCCACCCGAAGATCGGAAGAGCACACGTC1569110.26630630318104764No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC1564300.26548995931841163No Hit
GGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAAGATCGGAAGAGCACA1549580.262991709493463No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCTG1502460.25499459456468754Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTAGATCGGAAGAGCACACGTC1373060.23303307776113164No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGATCGGAAGAGCACACGTCTGAA1352830.229599681432415Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAGATCGGAAGAGCACACG1339700.22737128332089498No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGAGATCGGAAGAGCACACGTCTGA1304740.22143793998664218Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGATCGGAAGAGCACACG1281830.21754969926044845No Hit
GTCAACAAGTACCGTAAGGGAAAGTTGAAGATCGGAAGAGCACACGTCTG1213730.2059918994588862Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAATACAAGCTTGGGCTGCAGGTCGACCCGTAGATCGGAAGAGCACACGT1178820.2000670420275714No Hit
GCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCAGATCGGAAGAGCACA1139040.19331565765009495No Hit
GGGTCGGCGGCGACGTCGGCTACCCACCCGAGATCGGAAGAGCACACGTC1118400.18981267691728668No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTAGATCGGAAGAGCACACGTC1085880.18429344564640848No Hit
GGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGATCGGAAGAGCACAC1082230.18367397473193414No Hit
ATAAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGT1013980.1720907172215579No Hit
CAGGGAAGAGCCCAGCGCCGAATCCCCGCAGATCGGAAGAGCACACGTCT1011020.17158835176762802Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGACCTCAGATCAGACGTGGCGACCCGCAGATCGGAAGAGCACACGTCT985890.1673233369509869Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCGGGTTAAGGCGCCCGATGCCGACGCTAGATCGGAAGAGCACACGTC983010.16683454894175784No Hit
GTCAACAAGTACCGTAAGGGAAAGTTGAGATCGGAAGAGCACACGTCTGA955960.16224367545229734Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACGT935390.15875257498360226No Hit
GGGGTCGGCGGCGACGTCGGCTACCCACCCGAGATCGGAAGAGCACACGT888180.15074018542953835No Hit
GCGTCGGGCCCATACCCGGCCGTCGCCGGCAAGATCGGAAGAGCACACGT833900.14152788920004056No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGAGATCGGAAGAGCACACGTCTG821660.139450540160817Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACAGGGAAGAGCCCAGCGCCGAATCCCCAGATCGGAAGAGCACACGTCTG726810.12335278228742233Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT724160.12290302943170808No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGAAGATCGGAAGAGCACACGTC718100.12187453799562192No Hit
CGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACGT708700.12027918824327706No Hit
ACCGAACGCCGGGTTAAGGCGCCCGATGCAGATCGGAAGAGCACACGTCT688440.1168407003727976Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTC681320.11563230779442575Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACGCCGGGTTAAGGCGCCCGATGCCGACGCTCAGATCGGAAGAGCACACG665480.1129439737436659No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC663960.11268600229435055No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC660480.11209538344986543No Hit
CGCGACCTCAGATCAGACGTGGCGACCCAGATCGGAAGAGCACACGTCTG598690.10160850459907936Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)