FastQCFastQC Report
Sat 2 Sept 2023
SRR12414240_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12414240_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49051163
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length20-35
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT669184813.642587842412626No Hit
ACAAAGCATCTGGCCTACACCCAGAAGAA15870123.2354217574820807No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT15422633.144192524038625No Hit
CGACTGCATAATTTGTGGTAGTGGGG10146142.0684810266374316No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT8684411.7704799374481703No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT8336251.6995009883863508No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTT8135261.6585254054017027No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGG7200921.468042663942545No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT6850901.3966845189786836No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCC4605000.9388156607010522No Hit
GACCGCCTGGGAATACCGGGTGCTGTAGGCTT4161880.8484773337586307No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGG4121030.8401492947272219No Hit
TGGCAATTTTTGACAGTCTCTACGGAGACTG3827550.7803178897103826No Hit
TTGGCAATTTTTGACAGTCTCTACGGAGACTG3489820.7114652918627026No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGC3399460.693043710298979No Hit
ACAAAGCATCTGGCCTACACCCAGAAGAAT3029100.6175388746643989No Hit
CAACCCGCAGTTTTATCCGGTAAAGCG2937110.5987849870144771No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCT2874750.5860717308578391No Hit
TCGACTGCATAATTTGTGGTAGTGGGG2616940.533512324672098No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC2465580.5026547484715091No Hit
TCACAAAGCATCTGGCCTACACCCAGAAGAA1980030.40366626984970777No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGC1921060.3916441288048563No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCC1915430.39049634766050295No Hit
ACAACCCGCAGTTTTATCCGGTAAAGCG1853330.3778360973826451No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGA1787780.36447249986712854No Hit
ACAAAGCATCTGGCCTACACCCAGAAGA1779810.3628476658137545No Hit
CACAAAGCATCTGGCCTACACCCAGAAGAA1671570.34078091074007766No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC1553000.31660819132871526No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGAAGGCCC1533800.31269391104957084No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTT1403980.2862276680371473No Hit
CCGCCTGGGAATACCGGGTGCTGTAGGCTT1343910.2739812713513031No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGC1245100.2538369987272269No Hit
ATCACAAAGCATCTGGCCTACACCCAGAAGAA1227080.2501632835902382No Hit
CGACTGCATAATTTGTGGTAGTGGGGG1161360.23676502838475005No Hit
GACTGCATAATTTGTGGTAGTGGGG1133990.2311851402993238No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGAT1045470.21313867726235153No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCC1017250.20738550072706735No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGC924180.1884114348114437No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCT871610.17769405385964038No Hit
CAAAGCATCTGGCCTACACCCAGAAGAA846250.1725239419909371No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGC829290.1690663277443595No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCC674260.13746055317791342No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGA667530.1360885163925675No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG665220.13561757954648293No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGC619810.12635989894877722No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTG618480.12608875349194065No Hit
TGGAATGAGTCCACTTTAAATCCTTTAACGA526740.10738583303315356No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATT509810.10393433484951213No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)