FastQCFastQC Report
Sat 2 Sept 2023
SRR12414241_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12414241_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46377074
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length20-35
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT560411112.083795972121916No Hit
ACAAAGCATCTGGCCTACACCCAGAAGAA13520982.9154448165487974No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT12920002.785859237260203No Hit
CGACTGCATAATTTGTGGTAGTGGGG8833071.9046199421722896No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT7535331.6247963379492203No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTT7044041.5188625310859414No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGG6734311.4520773777146871No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT6660151.4360867181918375No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT5407081.1658950282201934No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCC3959420.853745106903467No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGG3726440.8035090786451944No Hit
GACCGCCTGGGAATACCGGGTGCTGTAGGCTT3506250.7560308785327854No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGC3313750.7145233008878481No Hit
TGGCAATTTTTGACAGTCTCTACGGAGACTG3244950.6996883848256575No Hit
TTGGCAATTTTTGACAGTCTCTACGGAGACTG2888710.6228745694478267No Hit
ACAAAGCATCTGGCCTACACCCAGAAGAAT2518470.5430420211503642No Hit
CAACCCGCAGTTTTATCCGGTAAAGCG2508670.5409289081066219No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCT2389320.5151942099667607No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC2332560.5029554042154536No Hit
TCGACTGCATAATTTGTGGTAGTGGGG2263970.48816576914705745No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCC1917000.41335078620958277No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGC1671260.36036339851884575No Hit
TCACAAAGCATCTGGCCTACACCCAGAAGAA1631680.35182900930748673No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGA1603090.3456643254380386No Hit
ACAAAGCATCTGGCCTACACCCAGAAGA1569800.33848620980271416No Hit
ACAACCCGCAGTTTTATCCGGTAAAGCG1533880.33074100362605885No Hit
CACAAAGCATCTGGCCTACACCCAGAAGAA1400370.3019530727617702No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGAAGGCCC1396560.3011315461600704No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC1295870.27942038775451855No Hit
CCGCCTGGGAATACCGGGTGCTGTAGGCTT1159180.2499467732699135No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTT1102470.23771874870760498No Hit
ATCACAAAGCATCTGGCCTACACCCAGAAGAA1075920.23199393734930326No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGC1038890.22400938877687712No Hit
GACTGCATAATTTGTGGTAGTGGGG995940.2147483474270067No Hit
CGACTGCATAATTTGTGGTAGTGGGGG985880.21257917220047132No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGC914870.19726772758453887No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGAT887420.1913488548242608No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCC880110.18977264499265303No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCT764840.16491769187508468No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGC709250.1529311659463467No Hit
CAAAGCATCTGGCCTACACCCAGAAGAA705650.15215492033844136No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG644780.13902990085144223No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCC608900.1312933196259859No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGA570450.12300258528599713No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGC562310.12124740771701122No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTG549930.1185779853209368No Hit
TGGAATGAGTCCACTTTAAATCCTTTAACGA470430.10143589481302767No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)