FastQCFastQC Report
Sat 2 Sept 2023
SRR12414242_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12414242_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42310466
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length20-35
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAAAGCATCTGGCCTACACCCAGAAGAA19026034.496766828330371No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGG13741933.247879614467021No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT13341423.1532198203631228No Hit
CGACTGCATAATTTGTGGTAGTGGGG8468602.001537870086328No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTT7194441.7003925222662402No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGC6440991.5223160151438653No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT5178961.2240375702787107No Hit
TGGCAATTTTTGACAGTCTCTACGGAGACTG4865091.149854979143931No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT3575760.8451242300191163No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGG3460560.8178969241321993No Hit
TTGGCAATTTTTGACAGTCTCTACGGAGACTG3404390.8046212490309136No Hit
GACCGCCTGGGAATACCGGGTGCTGTAGGCTT3170780.7494079597232515No Hit
CCGCCTGGGAATACCGGGTGCTGTAGGCTT2911930.6882292433271711No Hit
CAACCCGCAGTTTTATCCGGTAAAGCG2750550.6500873802713495No Hit
ACAAAGCATCTGGCCTACACCCAGAAGA2684550.6344884029403033No Hit
ACAAAGCATCTGGCCTACACCCAGAAGAAT2568380.6070318393562482No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGA2523800.5964954392135506No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT2508280.5928273160593409No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCG2175370.5141446563126958No Hit
TCACAAAGCATCTGGCCTACACCCAGAAGAA2131550.5037878807574466No Hit
CACAAAGCATCTGGCCTACACCCAGAAGAA1983490.4687941749447997No Hit
TCGACTGCATAATTTGTGGTAGTGGGG1901170.44933799594644036No Hit
GACTGCATAATTTGTGGTAGTGGGG1828630.43219330177077225No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCC1793790.4239589325251109No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC1637150.386937359659428No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT1503240.35528798004730083No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGG1500310.3545954799930589No Hit
ATCACAAAGCATCTGGCCTACACCCAGAAGAA1441900.3407903850550831No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCT1349820.31902744819685985No Hit
CGTGCTTTAAGGAAAGATTAAAAAAAGTA1094280.2586310441487456No Hit
CAAAGCATCTGGCCTACACCCAGAAGAA1062740.25117662376963656No Hit
GCTACATGTGGCTTAGAACATGTCCG1056850.2497845332169114No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCC1047960.24768339824004773No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGAAGGCCC1032630.24406018123270018No Hit
ACAACCCGCAGTTTTATCCGGTAAAGCG985360.23288800458969183No Hit
CGACTGCATAATTTGTGGTAGTGGGGG949430.22439601586992683No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGC904930.21387852357853968No Hit
CAACCCGCAGTTTTATCCGGTAAAGC867190.20495874472287778No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG848660.20057921366311587No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGC843570.19937620162349426No Hit
ACTGCATAATTTGTGGTAGTGGGG838830.19825591143335553No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGC824830.19494703745404268No Hit
TCGCTTCTGGCGCCAAGCGCCCGTCCGC815820.1928175407002135No Hit
CGTGCTTTAAGGAAAGATTAAAAAAAGT809380.1912954586697296No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCT795420.18799603861607198No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGA787690.18616906748320852No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC785530.18565855549782884No Hit
ATTGATCATCGACACTTCGAACGCAC734720.17364970643433708No Hit
ATAGAATTACCTATCCCTAACTCGTTAGCCCA624450.147587596884421No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGAT623110.14727089037497246No Hit
CAAAATGTAAACTTAAAATATAGCCAAAAGAG563840.13326253603541025No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGA506310.11966542746184833No Hit
ATTGATCATCGACACTTCGAACGCACT451410.10668991450011446No Hit
ACAACCCGCAGTTTTATCCGGTAAAGC445870.1053805457968721No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGC442330.10454387337638872No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCC431080.10188495678586948No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCT428110.10118300280597241No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)