FastQCFastQC Report
Sat 2 Sept 2023
SRR12414243_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12414243_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49317040
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length20-35
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAAAGCATCTGGCCTACACCCAGAAGAA20741484.205743085959742No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGG15761063.195864958643098No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT14472482.9345800153456088No Hit
CGACTGCATAATTTGTGGTAGTGGGG9445751.9153116245419433No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTT8175461.6577353385361326No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGC7579951.5369839714630076No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT5986051.2137893920640817No Hit
TGGCAATTTTTGACAGTCTCTACGGAGACTG5484091.1120071277594925No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT3912760.793389059846252No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGG3903290.7914688310571762No Hit
TTGGCAATTTTTGACAGTCTCTACGGAGACTG3805450.7716298463979185No Hit
GACCGCCTGGGAATACCGGGTGCTGTAGGCTT3621780.734387140834081No Hit
CCGCCTGGGAATACCGGGTGCTGTAGGCTT3382280.6858238045105708No Hit
CAACCCGCAGTTTTATCCGGTAAAGCG3177460.6442925203945735No Hit
ACAAAGCATCTGGCCTACACCCAGAAGA3047760.6179932940014242No Hit
ACAAAGCATCTGGCCTACACCCAGAAGAAT2865350.5810060782236728No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGA2849760.5778448990450359No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT2775550.5627973617232502No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCG2623550.5319763716557198No Hit
TCACAAAGCATCTGGCCTACACCCAGAAGAA2516680.510306376862845No Hit
CACAAAGCATCTGGCCTACACCCAGAAGAA2154260.4368185925189346No Hit
TCGACTGCATAATTTGTGGTAGTGGGG2131470.43219747170552003No Hit
GACTGCATAATTTGTGGTAGTGGGG2105130.4268565185582914No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCC2018250.4092398894986398No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC1889370.38310693423611797No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGG1796220.36421893933618077No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT1668290.338278615261581No Hit
ATCACAAAGCATCTGGCCTACACCCAGAAGAA1651110.3347950323052641No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCT1605160.32547776589998106No Hit
GCTACATGTGGCTTAGAACATGTCCG1372520.27830542952293974No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGAAGGCCC1291710.26191961236927436No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCC1279970.2595390964259007No Hit
CGTGCTTTAAGGAAAGATTAAAAAAAGTA1241180.2516736608685355No Hit
CAAAGCATCTGGCCTACACCCAGAAGAA1154360.23406919798917375No Hit
ACAACCCGCAGTTTTATCCGGTAAAGCG1130120.22915406115208858No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGC1094060.22184218679791No Hit
CGACTGCATAATTTGTGGTAGTGGGGG1057570.21444312148498776No Hit
ACTGCATAATTTGTGGTAGTGGGG1010560.204910919227918No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG1008430.20447901982762953No Hit
CAACCCGCAGTTTTATCCGGTAAAGC1007980.20438777347545597No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGC992830.20131581295227777No Hit
TCGCTTCTGGCGCCAAGCGCCCGTCCGC987270.20018841357875494No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGC951150.19286437304428652No Hit
ATTGATCATCGACACTTCGAACGCAC940990.1908042331818779No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGA940380.19068054368226478No Hit
CGTGCTTTAAGGAAAGATTAAAAAAAGT933090.1892023527770523No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCT928540.1882797507717414No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC881880.17881851789969552No Hit
ATAGAATTACCTATCCCTAACTCGTTAGCCCA728550.14772784416907422No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGAT718260.145641344249371No Hit
CAAAATGTAAACTTAAAATATAGCCAAAAGAG633640.12848297464730243No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGA579830.11757193862405367No Hit
ATTGATCATCGACACTTCGAACGCACT569350.11544691246676606No Hit
ATTGATCATCGACACTTCGAACGCA552600.11205052046919281No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGC537790.10904750163432356No Hit
ACAACCCGCAGTTTTATCCGGTAAAGC522730.10599379038158008No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCT522710.10598973498815013No Hit
CGCCTGGGAATACCGGGTGCTGTAGGCTT507970.10300091003028569No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCC496460.10066703111135623No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)