FastQCFastQC Report
Wed 30 Aug 2023
SRR12431995.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12431995.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70001943
Total Bases3.5 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAAAAA4086300.5837409398764831No Hit
CCGCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAA3693000.5275567851023792No Hit
GGGCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAA2651850.37882519918054275No Hit
CCGGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAAAAA2494670.3563715367157737No Hit
GCGCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAA2461550.3516402394716387No Hit
CGGCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAA2349420.3356221126605014No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA2287050.3267123599697797No Hit
CGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAAGATCGGAAG2213250.3161697954583918No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAG2210170.31572980767119563No Hit
GCCGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAAAAA2171410.3101928185050521No Hit
GCGGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAAAAA1945880.277975141347148No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAG1933490.2762051904759272No Hit
GGGGGGCCGCCGGTGAAATACCACTACTCTCATAAAAAAAAAAAAAAAGA1914790.27353383605366494No Hit
CGCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAG1808720.2583813994991539No Hit
CCCGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAAAAA1800820.25725285939563136No Hit
CCCCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAA1789520.25563861848806113No Hit
CCGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA1719280.24560461128914665No Hit
GGGGGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAAAAAAAGATC1713760.24481606174845746No Hit
GCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAG1696790.24239184332354888No Hit
CGCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA1677590.23964906231245608No Hit
CGCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAG1658570.2369319948733423No Hit
CCGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAG1623210.2318807065112464No Hit
CGGGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAAAAA1581720.22595372817008808No Hit
CCGCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAG1575540.22507089553214257No Hit
GGCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAG1547300.2210367217949936No Hit
GCGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA1424100.2034372103071482No Hit
CGCCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAA1400830.2001130168629748No Hit
GCGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAG1381890.1974073776780739No Hit
GCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA1365910.1951245838990498No Hit
GCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAG1365440.19505744290554905No Hit
GGCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA1352850.19325892139879602No Hit
GGCCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAA1344530.19207038296065584No Hit
GGCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAG1343280.19189181648857945No Hit
GCCCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAA1315100.18786621394209016No Hit
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA1312010.18742479762311742No Hit
CCGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAAGATCGGAA1261800.18025213957275443No Hit
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAG1251110.17872503910355747No Hit
GCGCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAG1231770.17596225864759213No Hit
GGGCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAG1227400.17533799026121316No Hit
CGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA1169730.16709964750549852No Hit
CGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAG1142640.1632297549226598No Hit
CCCCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAA1112950.15898844407790225No Hit
CGCGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAAAAA1100950.15727420594596925No Hit
CGGGGGGGTGGGGGCCGGGCCGCAAAAAAAAAAAAAAAGATCGGAAGAGC1093850.15625994838457555No Hit
CGGCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAG1052250.1503172561938745No Hit
GGCGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAAAAA1046540.14950156454942973No Hit
CCGGGGCCGCCGGTGAAATACCACTACTCTCATAAAAAAAAAAAAAAAGA1031540.1473587668845135No Hit
CCGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAA1022500.14606737415845727No Hit
CCGCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAAAAG978150.13973183572918826No Hit
CGGGGGGGGATGCGTGCATTTATCAAAAAAAAAAAAAAAGATCGGAAGAG977350.1396175531870594No Hit
GGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAA949210.1355976647676765No Hit
CCGGGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAAAAAAAGATC932890.13326630090824765No Hit
CCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAGA905810.12939783685718553No Hit
CGCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAGA890670.12723503974739672No Hit
GCGGGGCCGCCGGTGAAATACCACTACTCTCATAAAAAAAAAAAAAAAGA863910.12341228871318614No Hit
CGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAAA838290.11975239030150919No Hit
GCGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAA823410.11762673501791228No Hit
CGGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAGAT814230.11631534284698354No Hit
CGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAA799970.1142782565335365No Hit
GGGGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAAAAAAAAAAAAAAAGA785940.11227402645095151No Hit
CCGCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAAAAAAAAAAAAAA784270.1120354616442575No Hit
GCGGGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAAAAAAAGATC779170.11130691043818598No Hit
CCGCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAAAAAAAG778840.11125976888955783No Hit
GGGCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAAAAAAAAAAAAAA765400.10933982218179286No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAA760480.10863698454770034No Hit
CGGGGGCCGCCGGTGAAATACCACTACTCTCATAAAAAAAAAAAAAAAGA758360.10833413581105886No Hit
GGGCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAAAAAAAG745580.10650847220055021No Hit
CGCCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAA725080.103579982058498No Hit
CCGGGGGGGGATGCGTGCATTTATCAAAAAAAAAAAAAAAGATCGGAAGA719240.10274571950095727No Hit
CGCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAA707980.10113719272049349No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAA706480.10092291295400187No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)