FastQCFastQC Report
Wed 30 Aug 2023
SRR12431996.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12431996.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99120412
Total Bases4.9 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAA8197230.8269971678487373No Hit
CGCCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAA5550190.5599442020075542No Hit
GCCCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAA4334620.4373085132051307No Hit
GGCCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAA4295170.4333285055352676No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAAAAA4249400.428710889539079No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAG4052490.408845152903521No Hit
CCGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAA3981940.401727547298734No Hit
CCGCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAA3906420.3941085313487196No Hit
GGGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAA3117320.31449828921211503No Hit
CGCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAG3071790.30990488619034395No Hit
CGGGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCGCTCAAAAA3006380.30330584178766323No Hit
GGGGGGCCGCCGGTGAAATACCACTACTCTCATAAAAAAAAAAAAAAAGA2991770.3018318769700029No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA2966930.29932583411780006No Hit
CCGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAA2901620.29273687845446006No Hit
GGGCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAA2865810.28912410089659435No Hit
CGGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAA2781420.28061021376706946No Hit
GGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAA2780940.28056178781823465No Hit
CCGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAG2780420.28050932637366355No Hit
GGGGGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAAAAAAAGATC2668240.2691917785813885No Hit
GCGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAA2667990.269166556733037No Hit
CGGGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCGCTAAAAAA2661860.26854811701145875No Hit
GCGCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAA2620740.26439962739460765No Hit
CGGCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAA2568090.25908790613178645No Hit
GCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAG2540720.25632661817426666No Hit
GCCGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAAAAA2539620.25621564204152014No Hit
CCGGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAAAAA2514040.25363494251819696No Hit
GGCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAG2416370.2437812708042416No Hit
GCGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAA2383520.24046711993085743No Hit
CGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAA2357510.23784303882836969No Hit
GCGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAG2330130.23508074199691584No Hit
CGCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA2227230.22469942921544755No Hit
CCCCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAA2157550.21766959564292368No Hit
CCCCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAAAAAAA2144530.21635604178077872No Hit
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAG2131810.21507275413665553No Hit
CCGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA2110470.21291981716137337No Hit
CCCGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAAAAA2108270.21269786489588036No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAA2052290.20705018861301747No Hit
CGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAAA2025420.2043393443522006No Hit
CGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAG1986120.20037446979134832No Hit
GCGGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAAAAA1946030.19632989418970534No Hit
CCGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCGCTCAAAAAA1892490.19092838314675287No Hit
CGGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCGCTCAAAAAA1892440.19092333877708256No Hit
GCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA1816660.1832780921047826No Hit
GGGGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAAAAAAAAAAAAAA1797850.1813804002348174No Hit
GGCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA1751670.17672142040733244No Hit
GCGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA1725940.17412558777499834No Hit
CGCCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAA1717730.17329730227513582No Hit
CGGGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAAAAA1673430.16882799074725396No Hit
GGCCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAA1626830.1641266382145385No Hit
GCCCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAA1607490.16217547602606816No Hit
CCGGGGCCGCCGGTGAAATACCACTACTCTCATAAAAAAAAAAAAAAAGA1597220.16113936249578947No Hit
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA1589110.16032116573526753No Hit
GGGCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAAAAAAAAAAAAAA1510740.15241462071404627No Hit
GCGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCGCTCAAAAAA1510220.1523621592694752No Hit
CCGCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAAAAAAAAAAAAAA1492230.15054719506210285No Hit
CGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA1462770.1475750524523647No Hit
CGGGGGGGTGGGGGCCGGGCCGCAAAAAAAAAAAAAAAGATCGGAAGAGC1445820.14586501113413453No Hit
CCGGGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAAAAAAAGATC1438710.1451477017670185No Hit
CGCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAA1434450.1447179214711093No Hit
CGCCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAAAAAAA1433090.14458071461607727No Hit
CCGGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAAAAAAAAAAAAAA1397580.14099820327623336No Hit
GGGGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAAAAAAAAAAAAAAAGA1378180.1390409878441587No Hit
GCGGGGCCGCCGGTGAAATACCACTACTCTCATAAAAAAAAAAAAAAAGA1357030.13690721947362366No Hit
CGGCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAAAAAAAAAAAAAA1331450.13432651995030045No Hit
GCGCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAAAAAAAAAAAAAA1277690.12890281368079867No Hit
CGCGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAAAAA1270860.1282137527838363No Hit
CGGGGGCCGCCGGTGAAATACCACTACTCTCATAAAAAAAAAAAAAAAGA1228670.12395731365604089No Hit
GCGGGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAAAAAAAGATC1226450.12373334364267977No Hit
CCGGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCGCTCAAAAA1204190.12148759026546418No Hit
GGCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAA1186600.1197129810154542No Hit
GGCGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAAAAA1181620.11921056179629277No Hit
CGGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCGCTAAAAAAA1171380.11817747488781624No Hit
GCGGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAAAAAAAAAAAAAA1168840.11792122090856522No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAG1160420.11707174905608746No Hit
GGCCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAAAAAAA1130000.11400275454867964No Hit
CCGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCGCTCTAAAAA1129380.11394020436476797No Hit
GCCCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAAAAAAA1123690.11336615509628833No Hit
CCCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAAA1111530.11213936439247246No Hit
CCGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAAAAAAA1109960.11198097118482518No Hit
CGGGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAAAAAAAAAAAAAA1107430.11172572607950823No Hit
CGCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAG1100340.11101043446026032No Hit
CGGGGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAAAAAAAGATC1098840.11085910337015144No Hit
CGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAAGATCGGAAG1089720.10993901034228955No Hit
CCGGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAAAAAAAAAAAAAAAGA1082170.10917731052207491No Hit
CGCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAAAAAAAAAAAAAAA1076620.1086173854886721No Hit
CCGGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCGCTAAAAAA1070040.10795354644006121No Hit
CCGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCGCTAAAAAAA1047640.10569366882776879No Hit
GCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAG1046330.10556150634240705No Hit
GGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAAA1039720.10489464067199399No Hit
CCCCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAAAAAAAAAAAAAA1022530.10316038637934637No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)