FastQCFastQC Report
Wed 30 Aug 2023
SRR12431997.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12431997.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81397328
Total Bases4 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAAAAA6390040.7850429684866314No Hit
CCGGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAAAAA3902620.4794530847499073No Hit
CGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAAGATCGGAAG3703050.4549350809156782No Hit
CCCCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAA3636630.4467751079003478No Hit
CCGCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAA3619060.44461656038635566No Hit
GCCGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAAAAA3503120.43037284958543115No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA3387270.41614019565851107No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAG3163710.3886749206313013No Hit
GCGGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAAAAA3042140.37373954093431666No Hit
CCCGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAAAAA2900790.3563741060394513No Hit
GGGGGGCCGCCGGTGAAATACCACTACTCTCATAAAAAAAAAAAAAAAGA2820500.34651014588586987No Hit
GGGCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAA2641110.32447133891176383No Hit
CCGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA2576350.3165153037947388No Hit
GGGGGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAAAAAAAGATC2505720.3078381147843084No Hit
CGGGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAAAAA2491950.30614641306161794No Hit
CGCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA2488290.3056967668521011No Hit
GCGCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAA2416470.2968733813964999No Hit
CGCCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAA2393990.2941116199784838No Hit
CCGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAG2330570.2863202094299705No Hit
CGGCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAA2316040.2845351385490197No Hit
CGCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAG2306250.2833323963656399No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAG2190590.26912308472828494No Hit
GCGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA2098720.25783647345278954No Hit
CCGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAA2062450.25338055322897085No Hit
GCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA2051900.25208444188733076No Hit
CGCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAG2040670.25070478971987875No Hit
GGCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA2003070.24608547346910456No Hit
GCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAG1965480.24146738575988638No Hit
CCGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAAGATCGGAA1959760.24076465998982177No Hit
GCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAG1950960.23968354342049164No Hit
GCGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAG1930260.23714046239945372No Hit
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA1923720.23633699622179244No Hit
GGCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAG1902410.233718974165835No Hit
GGCCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAA1866890.22935519455872067No Hit
GCCCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAA1865130.22913897124485463No Hit
CCCCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAA1807450.22205274354951798No Hit
GGCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAG1780750.21877253759484588No Hit
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAG1772030.2177012493579642No Hit
CCGCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAG1747790.21472326462608207No Hit
CGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA1731620.21273671292993795No Hit
CGCGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAAAAA1721880.21154011345433846No Hit
GGCGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAAAAA1647270.2023739649045974No Hit
CGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAG1606100.19731605931831078No Hit
CGGGGGGGGATGCGTGCATTTATCAAAAAAAAAAAAAAAGATCGGAAGAG1592100.19559610113983103No Hit
GGGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAA1563280.19205544437527483No Hit
CCGGGGCCGCCGGTGAAATACCACTACTCTCATAAAAAAAAAAAAAAAGA1525970.18747175582962625No Hit
CGCCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAA1404240.1725167194677447No Hit
CGGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAA1390150.17078570441526042No Hit
GGGCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAG1372230.1685841579468063No Hit
GCGCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAG1369460.16824385193577854No Hit
CCGGGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAAAAAAAGATC1356860.16669588957514675No Hit
GCGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAA1350180.1658752238157007No Hit
GGCCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAA1330710.16348325340605777No Hit
CGGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAGAT1328840.1632535161350751No Hit
CGGGGGGGTGGGGGCCGGGCCGCAAAAAAAAAAAAAAAGATCGGAAGAGC1327760.16312083364702093No Hit
GCCCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAA1326530.16296972303562593No Hit
GCGGGGCCGCCGGTGAAATACCACTACTCTCATAAAAAAAAAAAAAAAGA1271420.15619923052019596No Hit
CCCCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAAAAAAA1210040.14865844244911822No Hit
CGGCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAG1148210.14106237000801797No Hit
GCGGGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAAAAAAAGATC1136920.13967534659122963No Hit
CGGGGGCCGCCGGTGAAATACCACTACTCTCATAAAAAAAAAAAAAAAGA1125240.1382404100537551No Hit
CCGGGGGGGGATGCGTGCATTTATCAAAAAAAAAAAAAAAGATCGGAAGA1102990.1355069050915283No Hit
GGGGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAAAAAAAAAAAAAAAGA1044420.1283113371977026No Hit
CCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAGA1032910.12689728586668103No Hit
CGGGGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAAAAAAAGATC1000530.12291926830816854No Hit
CGCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAGA982150.12066120892813582No Hit
CCGCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAAAAG954790.11729991923076392No Hit
GGGGGGCCGCCGGTGAAATACCACTACTCTCATAAAAAAAAAAAAAAAAA909770.11176902514539544No Hit
CCGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAA894560.10990041343863279No Hit
CCGCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAAAAAAAAAAAAAA870210.10690891474963406No Hit
GGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAAGATCGGAAGA865570.10633887146762361No Hit
CGGGGCCGCCGGTGAAATACCACTACTCTCATAAAAAAAAAAAAAAAGAT848500.10424175103143435No Hit
CCGGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAAAAAAAAAAAAAAAGA848500.10424175103143435No Hit
GGGCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAAAAAAAAAAAAAA841280.10335474402796122No Hit
GGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAA839680.10315817737899209No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAA835840.10268641742146621No Hit
CGGGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAAAAAAAGATCG834790.10255742055808025No Hit
CCGCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAAAAAAAG829960.10196403498650472No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)