FastQCFastQC Report
Wed 30 Aug 2023
SRR12431998.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12431998.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101471691
Total Bases5 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAAAAAAAGATC4111680.4052046397847061No Hit
CCCCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAA4088050.402875911469732No Hit
GGGATGTTAGGGATAACAGGGTAATGCAAAAAAAAAAAAAAAGATCGGAA3981060.3923320840292294No Hit
GGGATGTTAGGGATAACAGGGTAATGCGAAAAAAAAAAAAAAAGATCGGA3853150.3797265978350553No Hit
CGGATGTTAGGGATAACAGGGTAATGCAAAAAAAAAAAAAAAGATCGGAA3458470.3408310205454248No Hit
CGGATGTTAGGGATAACAGGGTAATGCGAAAAAAAAAAAAAAAGATCGGA3385480.3336378813279065No Hit
CGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAAGATCGGAAG3266290.32189174811327426No Hit
CCGATGTTAGGGATAACAGGGTAATGCAAAAAAAAAAAAAAAGATCGGAA3147540.3101889767462336No Hit
CCGATGTTAGGGATAACAGGGTAATGCGAAAAAAAAAAAAAAAGATCGGA3037940.29938793470979014No Hit
CGCCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAA2720450.2680994051828702No Hit
GCGATGTTAGGGATAACAGGGTAATGCAAAAAAAAAAAAAAAGATCGGAA2650580.2612137408846375No Hit
GCGATGTTAGGGATAACAGGGTAATGCGAAAAAAAAAAAAAAAGATCGGA2503990.24676734716089438No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAAAAA2401930.2367093695127245No Hit
CGGGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCGCTCTAAAA2208120.21760946114517793No Hit
GGGGGGCCGCCGGTGAAATACCACTACTCTCATAAAAAAAAAAAAAAAGA2193540.21617260719543935No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAG2155940.21246714021943325No Hit
CCGCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAA2142500.21114263287481827No Hit
CCGGGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAAAAAAAGATC2090470.20601509439711613No Hit
CCGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAA2089520.20592147222617982No Hit
GGCCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAA2087010.2056741125956007No Hit
GCCCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAA2073010.2042944174449601No Hit
CCGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAAGATCGGAA1926780.18988350159651918No Hit
CGGGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCGCTCAAAAA1848940.1822123965589575No Hit
GCGGGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAAAAAAAGATC1799410.17733123221529834No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAG1684660.1660226594627264No Hit
CGGGGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAAAAAAAGATC1668780.16445769096328552No Hit
CCGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAA1664770.1640625068522806No Hit
CGCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAG1643840.16199986260207294No Hit
GGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAA1621840.15983177022249487No Hit
GGGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAA1591490.1568407882352133No Hit
CGCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAG1591050.15679742638762176No Hit
GGGCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAA1570500.1547722310057886No Hit
GCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAG1497410.14756923682290857No Hit
CGGGGGGGTGGGGGCCGGGCCGCAAAAAAAAAAAAAAAGATCGGAAGAGC1452490.14314238638242463No Hit
GCGCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAA1450410.1429374031029009No Hit
CGGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAA1448590.1427580427333176No Hit
CCGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAG1421420.1400804486445387No Hit
CCGCGCGCCGGGCCGTACCCATATCCGCAGCAAAAAAAAAAAAAAAGATC1420840.14002328984544074No Hit
CCGGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAAAAA1399530.13792319672685852No Hit
CGGCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAA1389640.13694854065258458No Hit
GCGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAA1382190.13621434573313654No Hit
GCGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAA1377900.13579156771911882No Hit
GGCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAG1365460.13456561002812104No Hit
CGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAA1365080.13452816115974653No Hit
GGGGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAAAAAAAAAAAAAAAGA1358980.1339270082726817No Hit
GCCGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAAAAA1319140.13000079007257304No Hit
GCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAG1296720.12779130683847575No Hit
CGCATGTTAGGGATAACAGGGTAATGCGAAAAAAAAAAAAAAAGATCGGA1257850.1239606818023758No Hit
CGGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCGCTCTAAAAA1256080.12378624891547338No Hit
CCGCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAG1248570.12304614101680833No Hit
CGCATGTTAGGGATAACAGGGTAATGCAAAAAAAAAAAAAAAGATCGGAA1230380.12125352281751173No Hit
CCGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCGCTCTAAAAA1228960.12111358230937533No Hit
GGCATGTTAGGGATAACAGGGTAATGCAAAAAAAAAAAAAAAGATCGGAA1205720.11882328835931197No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAA1204960.11874839062256291No Hit
GGCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAG1200660.11832462711200901No Hit
GGCATGTTAGGGATAACAGGGTAATGCGAAAAAAAAAAAAAAAGATCGGA1200260.11828520725056214No Hit
GCGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAG1167560.11506263357728018No Hit
CCCCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAA1160650.11438165547078545No Hit
CGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAAA1127440.11110882147415875No Hit
CCCGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAAAAA1121280.11050175560787688No Hit
CCGGGGCCGCCGGTGAAATACCACTACTCTCATAAAAAAAAAAAAAAAGA1105800.10897620696988287No Hit
GCCATGTTAGGGATAACAGGGTAATGCGAAAAAAAAAAAAAAAGATCGGA1105740.10897029399066582No Hit
GCCATGTTAGGGATAACAGGGTAATGCAAAAAAAAAAAAAAAGATCGGAA1093280.10774236530659571No Hit
GCGGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAAAAA1088900.10731071782375244No Hit
CGGGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAAAAAAAGATCG1088100.10723187810085869No Hit
GCGCGCGCCGGGCCGTACCCATATCCGCAGCAAAAAAAAAAAAAAAGATC1074800.10592116770775013No Hit
CCGGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAAAAAAAAAAAAAAAGA1061960.10465579015530549No Hit
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAG1034730.10197228308730955No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)