FastQCFastQC Report
Wed 30 Aug 2023
SRR12431999.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12431999.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96987322
Total Bases4.8 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAA8076670.832755233720135No Hit
CGCCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAA5651310.5826854359376992No Hit
CCGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAA4329050.4463521531195592No Hit
CGGGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCGCTCTAAAA4290150.44234131962113565No Hit
GGCCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAA4270280.44029259824289196No Hit
GCCCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAA4170630.4300180594737939No Hit
CCGCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAA3604830.37168053779235183No Hit
GGGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAA3226950.3327187444148628No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAAAAA2985750.3078495145994442No Hit
CGGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAA2913020.3003505963387668No Hit
GCGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAA2811820.2899162428672894No Hit
CGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAAGATCGGAAG2795440.2882273623350483No Hit
GGGCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAA2576020.26560378685370856No Hit
CGGGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCGCTCAAAAA2558980.2638468561901317No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAG2434200.25098125711729624No Hit
GCGCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAA2351890.2424945808896548No Hit
CGGCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAA2308710.23804245260014498No Hit
CGGGGGGGTGGGGGCCGGGCCGCAAAAAAAAAAAAAAAGATCGGAAGAGC2133360.21996276997935876No Hit
GGGGGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAAAAAAAGATC2108340.21738305136417727No Hit
CGGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCGCTCTAAAAA2105360.21707579471057054No Hit
CCCCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAAAAAAA1940770.20010553544307577No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAG1919670.19792999336552464No Hit
GGGGGGCCGCCGGTGAAATACCACTACTCTCATAAAAAAAAAAAAAAAGA1888210.19468627043852185No Hit
CCGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCGCTCTAAAAA1854800.1912414903052999No Hit
CCCCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAA1854520.19121262055261204No Hit
CCGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAAGATCGGAA1853490.19110642110522447No Hit
CGCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAG1837290.18943609969971126No Hit
CCGGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAAAAA1822810.18794312106070937No Hit
CGCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAG1798730.18546032232955148No Hit
CCGGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCGCTCTAAAA1777320.18325281731152449No Hit
CCGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAG1753210.18076692539257863No Hit
GCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAG1732640.17864602963261528No Hit
GCCGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAAAAA1709410.17625087122211705No Hit
GGGGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAAAAAAAAAAAAAAAGA1690910.17434340541952484No Hit
CGCCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAA1608390.16583507687736754No Hit
CCGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAA1582830.1631996808820023No Hit
GGCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAG1572660.16215109022187457No Hit
CCGCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAG1545440.159344537835574No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGAAAAAAAAAAA1498200.1544737981320899No Hit
GCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAG1485910.15320662220161105No Hit
GGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAA1472760.15185077488787657No Hit
GGCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAG1439920.1484647653226264No Hit
GCGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCGCTCTAAAAA1436990.14816266398199962No Hit
GCGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAG1429440.14738421172202282No Hit
GCGGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAAAAA1403150.14467354815714986No Hit
CCCGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAAAAA1396740.14401263703311656No Hit
GGCCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAA1395650.143900251210153No Hit
GCGGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCGCTCTAAAA1392390.14356412480385838No Hit
CCGGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAAAAAAAAAAAAAAAGA1391710.14349401254733066No Hit
GCCCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAA1379940.14228045187184363No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGAAAAAAAAAA1336770.13782935464492976No Hit
CGCCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAAAAAAA1324200.13653330896176305No Hit
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAG1308510.13491557174864566No Hit
CCCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAAA1248590.12873744467343887No Hit
CCGGGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAAAAAAAGATC1245670.12843637439540811No Hit
CGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAA1243580.12822088231284498No Hit
GCGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAA1242660.12812602455401337No Hit
GGGCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAG1227490.12656190259588773No Hit
CGGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCGCTCAAAAAA1199690.12369554857901945No Hit
GCGCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAG1192100.12291297206865862No Hit
CGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAG1191810.12288307125337475No Hit
CGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAAA1178600.1215210375640643No Hit
CGGGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCGCTAAAAAA1176780.12133338417159308No Hit
CGGGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAAAAA1170950.12073227467812751No Hit
GCGGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAAAAAAAAAAAAAAAGA1141040.1176483664535041No Hit
CCGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAAAAAAA1112690.11472530399385603No Hit
CCGGGGCCGCCGGTGAAATACCACTACTCTCATAAAAAAAAAAAAAAAGA1102360.11366021633219237No Hit
CGGGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAAAAAAAAAAAAAAAGA1086260.11200020555263913No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA1078490.11119906991555041No Hit
CCGGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCGCTCAAAAA1070830.11040927596701762No Hit
CGGCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAG1069500.11027214464175018No Hit
CGGGGGGGGATGCGTGCATTTATCAAAAAAAAAAAAAAAGATCGGAAGAG1057820.1090678635296271No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAA1045170.10776356934569242No Hit
GCGGGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAAAAAAAGATC1040750.10730783967826227No Hit
CCGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCGCTCAAAAAA1031590.10636338634033013No Hit
GGCCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAAAAAAA1021290.10530139186645446No Hit
CGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAAA1000040.1031103838499634No Hit
GCCCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAAAAAAA997660.1028649909521164No Hit
CCGCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAAAAAAAAAAAAAA977290.10076471644407296No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)