FastQCFastQC Report
Wed 30 Aug 2023
SRR12432003.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12432003.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54516603
Total Bases2.7 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA3879540.7116254107028642No Hit
CCCCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAA3486690.6395647946002798No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAG3103730.569318304737366No Hit
CGCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA2888920.5299156295560089No Hit
CCGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA2771070.508298361877023No Hit
GCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA2456160.450534307869476No Hit
CGCCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAA2414450.4428834276413004No Hit
CGCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAG2347590.4306192739118393No Hit
GCGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA2341950.42958472669326075No Hit
GGCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA2290400.4201288917433098No Hit
CCGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAG2145730.39359202186533887No Hit
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA2115380.3880249105029526No Hit
GCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAG2027890.37197658848993215No Hit
GCCCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAA1952570.3581606139326033No Hit
CGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA1940240.355898917619647No Hit
CGGGGGGGTGGGGGCCGGGCCGCAAAAAAAAAAAAAAAGATCGGAAGAGC1912410.3507940507591788No Hit
GGCCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAA1903780.3492110467704673No Hit
GGCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAG1865090.3421141262231618No Hit
GCGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAG1825490.3348502840501636No Hit
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAG1652540.30312600365066766No Hit
CCGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAA1645060.30175394457354576No Hit
CGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAG1554000.285050776182808No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAAAAA1384850.2540235311433473No Hit
GGGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAA1331390.24421734420979974No Hit
CGGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAGAT1217800.22338148985548492No Hit
CGGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAA1193550.21893330367631306No Hit
CCCCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAAAAAAA1176740.2158498393599469No Hit
GCGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAA1151700.2112567431980309No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAG1069790.19623196258211467No Hit
CGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAAGATCGGAAG1066530.19563397961534765No Hit
GGGGGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAAAAAAAGATC1053870.19331175128428307No Hit
CGGGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCGCTCTAAAA1015020.18618548187971287No Hit
CCGCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAA1013880.18597637127170233No Hit
CGCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAG1012660.18575258623505944No Hit
GCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAG988230.18127138259146483No Hit
GGGCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAAAAAAAG988220.1812695482878858No Hit
CCGCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAAAAAAAG972620.17840803470458347No Hit
CCGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAAGATCGGAA940890.17258778944828973No Hit
GGGGGGCCGCCGGTGAAATACCACTACTCTCATAAAAAAAAAAAAAAAGA909470.16682440760294623No Hit
CGGCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAAAAAAAG890430.16333189358845415No Hit
GGCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAG881550.16170303201026667No Hit
GCCGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAAAAA859680.1576914100829063No Hit
CCGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAA856430.1570952614197183No Hit
GCGCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAAAAAAAG846680.15530681543015437No Hit
GGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAA836950.1535220380477485No Hit
CGGGGGGTGGGGGCCGGGCCGCAAAAAAAAAAAAAAAGATCGGAAGAGCA835630.1532799099753152No Hit
CCGCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGAAAAAA827560.15179962698702998No Hit
CCGCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAG803210.1473330977720677No Hit
CCGGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAAAAA792210.14531536383512378No Hit
CGCCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAAAAAAA790890.1450732357626905No Hit
CGCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAAAAAAAGA755070.1385027603425694No Hit
CCCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAA752030.13794513205454126No Hit
GGGCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAA732250.13431687957520022No Hit
GCGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAA727480.1334419167679982No Hit
CCGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCGCTCTAAAAA713820.13093625807902962No Hit
CGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAA704690.12926153891136613No Hit
CCGGGGGTGGGGGCCGGGCCGCAAAAAAAAAAAAAAAGATCGGAAGAGCA698860.12819213992478584No Hit
CGGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCGCTCTAAAAA697500.1279426746380364No Hit
CCCGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAAAAA693800.12726398231379166No Hit
GGGCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAAAAAAAAAAAAAA690320.12662564466828571No Hit
GCGCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAA687720.12614872573773536No Hit
GCGCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGAAAAAA687040.12602399309436063No Hit
CGCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAA683140.12530861469853505No Hit
CGGGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAAAAGATC681060.12492707955409474No Hit
CGGGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCGCTCAAAAA676170.1240301051039442No Hit
CCGCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAAAAAAAAAAAAAA669900.12287999675988616No Hit
GCCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAA667380.12241775225796808No Hit
CGGCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAA660610.12117592873495805No Hit
GCCCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAAAAAAA658270.12074670169746271No Hit
CCCCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAAAAAAAG656660.12045137882123727No Hit
GGGGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAAAAAAAAAAAAAA646020.11849967981313876No Hit
GCGCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAG640050.11740460057645191No Hit
GGCCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAAAAAAA639470.11729821096886761No Hit
GCGGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAAAAA632730.11606189035659467No Hit
GGGCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAG629550.11547858181845996No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAA625440.11472468304747453No Hit
GGGCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGAAAAAA622330.11415421463439313No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAGA621060.11392125807985504No Hit
CGGCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAAAAAAAAAAAAAA610450.11197506198249367No Hit
CGGGGGGGTGGGGGCCGGGCCGCAAAAAAAAAAAAAAAAAAAAAAAAAAA608350.11158985823089528No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAA601040.11024898231461706No Hit
CCGGGGGGTGGGGGCCGGGCCGCAAAAAAAAAAAAAAAGATCGGAAGAGC600200.11009490081397771No Hit
CCGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAAAAAAA589320.10809917851998226No Hit
GCGCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAAAAAAAAAAAAAA587620.10778734691154546No Hit
CGGCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGAAAAAA587060.10768462591111921No Hit
GCGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCGCTCTAAAAA581010.10657487224580006No Hit
CGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAAA579070.10621901735146631No Hit
GGCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAA577790.10598422649334918No Hit
CGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAA573090.10512210381120042No Hit
GCGGGGGTGGGGGCCGGGCCGCAAAAAAAAAAAAAAAGATCGGAAGAGCA569860.10452962375517051No Hit
CCCCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAA561850.10306034658835951No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGAAAAAAAAAAA553570.10154154322491445No Hit
CCGGGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAAAAAAAGATC552750.10139113033143317No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAAAAAAAG551200.10110681327668196No Hit
CGGGGGGGTGGGGGCCGGGCCGAAAAAAAAAAAAAAAGATCGGAAGAGCA550100.10090503988298756No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)