FastQCFastQC Report
Wed 28 Jun 2023
SRR1248253.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1248253.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50374980
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAACTGTAGGCACCATCAATAGATCGGAAGAGCACAC34688436.886043428702106No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA25868925.1352715177256645Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA8538561.695000176675008No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC5386001.0691815659281652No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGCTGTAGGCACCATC4127320.8193194319878638No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG3434790.681844439441961No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATACTGTAGGCACCATCA2933060.5822453924547464No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCA2620110.5201212983111855No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAAT2595430.5152220407829442No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA2293700.4553252428090294No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT2146790.42616195579630994No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCCTGTAGGCAC2085780.41405078473480283No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCCTGTAGGCACC1958950.38887360352301875No Hit
TTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCAT1937150.3845460583805691No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA1860370.36930436498436325No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCA1528380.3034006167347362No Hit
GCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAATAGATCGGAA1473790.292563887866556No Hit
CGCTTCTGGCGCCAAGCGCCCGGCTGTAGGCACCATCAATAGATCGGAAG1456520.28913559866425753No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA1441720.28619763223727335No Hit
TAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGCTGTAGGCACCAT1278420.253780745917914No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAA1246830.24750977568626328No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA1245190.24718421724435422No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGA1223720.2429221808127765No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGAT1218560.2418978627882334No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG1187180.2356685799180466No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGA1105440.21944227074631095No Hit
TGTAGGCACCATCAACTGTAGGCACCATCAATAGATCGGAAGAGCACACG1099360.21823532237630663No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACATCAAT1061280.21067601416417436No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG993070.19713556213818845No Hit
CCGCGGACGCTACGCCGCGACGAGTCTGTAGGCACCATCAATAGATCGGA992780.19707799387711916No Hit
ACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAAGAGC957210.1900169488901038No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG938930.18638816333028815No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGTACCATCAA910200.18068493525952764No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAATA850440.16882190325435364No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACTATCAA832330.16522686460619934No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCATC825220.16381544965377653No Hit
CTGTAGGCACCATCAACTGTAGGTACCATCAATAGATCGGAAGAGCACAC797890.1583901373261091No Hit
TTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATC797870.1583861671012078No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACACTGTAGGCACCATCAA797450.15830279237827985No Hit
CTGTAGGCACCATCAACTGTAGGCATCATCAATAGATCGGAAGAGCACAC793770.15757227099643514No Hit
GGTTGGCCTCGGATAGCCGGTCCTGTAGGCACCATCAATAGATCGGAAGA779020.15464423013170428No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGGCTGTAGGCACCAT759290.15072760326654222No Hit
GTTGGCCTCGGATAGCCGGTCCTGTAGGCACCATCAATAGATCGGAAGAG742870.1474680486225503No Hit
TTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA737770.14645564127271116Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAATAGATCGGA729500.14481395327601124No Hit
ATGCTAACTAGTTACGCGACCCCCTGTAGGCACCATCAATAGATCGGAAG724810.14388293553664933No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCATCATCAA712270.14139360452351543No Hit
TTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGG664410.1318928563346328No Hit
CTGTAGGCACCATCAACTGTAGGCACTATCAATAGATCGGAAGAGCACAC652910.12960997701636803No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATACTGTAGGCACCATCAA647450.12852610561830496No Hit
CTGTAGGCACCATCAATCTGTAGGCACCATCAATAGATCGGAAGAGCACA635750.12620352405102692No Hit
CGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCCTGTAGGCACC609720.12103627634194594No Hit
GTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATC592980.11771319809953273No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAA582250.11558317243996921No Hit
TCAACTTCTTAGAGGGACCTGTAGGCACCATCAATAGATCGGAAGAGCAC572360.11361989622626152No Hit
CTGTAGGCACCATCATCTGTAGGCACCATCAATAGATCGGAAGAGCACAC569740.1130997967641873No Hit
CTGTAGGCACCATCAACTGTAGGCACATCAATAGATCGGAAGAGCACACG550470.10927448507175586No Hit
CTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCAT539740.10714445941219233No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA529430.1050978084755567No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACATCA528850.10498267195341814No Hit
TTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCG518590.10294594657903587No Hit
CTGTAGGCACCACAACTGTAGGCACCATCAATAGATCGGAAGAGCACACG518090.10284669095650262No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATACTGTAGGCACCATC514110.10205661620113794No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)