FastQCFastQC Report
Wed 28 Jun 2023
SRR1248254.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1248254.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68317045
Total Bases3.4 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA27710074.056099030629911Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTGTAGGCACCATCAACTGTAGGCACCATCAATAGATCGGAAGAGCACAC11569561.6935100164241588No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA9189801.3451694229456206No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC6906741.0109834229510366No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG6249260.9147438973685118No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGCTGTAGGCACCATC5195370.7604793210830474No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCCTGTAGGCAC5066160.7415660323130193No Hit
TTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGG4261190.6237374581994873No Hit
GCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAATAGATCGGAA4228630.6189714440956865No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA3950340.5782363683909337No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATACTGTAGGCACCATCA3208340.469625113322744No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAAT3180190.4655046189424616No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGAT3085330.45161935795085983No Hit
TTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATC2992950.43809711031851567No Hit
TTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCG2946150.43124669692607465No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA2936450.42982684628704304No Hit
AGCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAATAGATCGGA2531330.37052685753606585No Hit
TAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGCTGTAGGCACCAT2510790.3675202872138278No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCA2494270.365102149836838No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCCTGTAGGCACC2488020.3641872976209671No Hit
CGCTTCTGGCGCCAAGCGCCCGGCTGTAGGCACCATCAATAGATCGGAAG2472370.3618965076724264No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGA2318200.3393296650930965No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGA2306740.33765219207007563No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA2141330.31344007926572354No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG2127910.31147570858780554No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCA2108970.3087033404328305No Hit
TTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCAT2010630.2943086897274319No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT2004220.2933704172948347No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA1948100.28515577627808697No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCTGTAGGCACCATCAATAGATCGGAA1825350.2671880787583831No Hit
CGGTTGGCCTCGGATAGCCGGTCCTGTAGGCACCATCAATAGATCGGAAG1821110.2665674430151363No Hit
ATGCTAACTAGTTACGCGACCCCCTGTAGGCACCATCAATAGATCGGAAG1778810.2603757232181222No Hit
TTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGA1747940.2558570851534928No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG1707920.24999910344482845No Hit
ACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAAGAGC1650020.24152391251700073No Hit
GGTTGGCCTCGGATAGCCGGTCCTGTAGGCACCATCAATAGATCGGAAGA1646750.24104526183765704No Hit
GTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATC1625220.2378937789244251No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAA1595510.2335449374310613No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG1549750.22684675544734112No Hit
TCGGTTGGCCTCGGATAGCCGGTCCTGTAGGCACCATCAATAGATCGGAA1420980.2079978722733104No Hit
GCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCCTGTAGGCAC1417360.20746798986987802No Hit
CGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAATAGATCGGAAG1302290.19062446275303624No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGGCTGTAGGCACCAT1287980.18852981711957828No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTCTGTAGGCA1197190.17524030789095169No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1172760.17166433354955563No Hit
CCGGGGTCCGGTGCGGAGTGCCCTTCTGTAGGCACCATCAATAGATCGGA1093190.16001716701886037No Hit
CCGCGGACGCTACGCCGCGACGAGTCTGTAGGCACCATCAATAGATCGGA1073290.15710427756352752No Hit
GCGAGCGCACGGGGTCGGCGGCGACCTGTAGGCACCATCAATAGATCGGA958130.1402475765747772No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAA946970.13861401645811816No Hit
GTTGGCCTCGGATAGCCGGTCCTGTAGGCACCATCAATAGATCGGAAGAG922800.13507609996890235No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA911050.1333561778030651No Hit
TTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAAG897700.13140205346996492No Hit
CCGGGGTCCGGTGCGGAGTGCCCTCTGTAGGCACCATCAATAGATCGGAA891520.1304974475989118No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACATCAAT878850.12864285918689838No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCATC875180.128105657965739No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA874430.1279958756998345No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAATA869980.12734450092213445No Hit
CTCGGTTGGCCTCGGATAGCCGGTCCTGTAGGCACCATCAATAGATCGGA801440.11731186558200812No Hit
TCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAAGA798690.11690933060702494No Hit
CGCTTCTGGCGCCAAGCGCCCGGCCTGTAGGCACCATCAATAGATCGGAA780580.1142584548263175No Hit
CGGTTGGCCTCGGGTAGCCGGTCCTGTAGGCACCATCAATAGATCGGAAG756940.11079811780500752No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCTGTAGGCACCATCAATAGATCGGA756060.11066930661301291No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAGA723150.10585206078512326No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGTACCATCAA696500.10195113093664984No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATACTGTAGGCACCATC684210.10015216553936138No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)