FastQCFastQC Report
Wed 28 Jun 2023
SRR1248255.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1248255.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58390098
Total Bases2.9 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA26491594.5370004345599835Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTGTAGGCACCATCAACTGTAGGCACCATCAATAGATCGGAAGAGCACAC12629772.162998596097578No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC10303561.7646074168260515No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA8936181.5304272995054744No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGCTGTAGGCACCATC8353781.4306843602146377No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG6567361.1247386500361758No Hit
ACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAAGAGC6220851.0653946838725976No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA4518280.7738092852661421No Hit
TAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGCTGTAGGCACCAT4032730.690653062442197No Hit
TTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGG3991790.68364159964246No Hit
GCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAATAGATCGGAA3873410.6633676141458095No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA3354030.5744176007377142No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAAT3239020.5547207678945838No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCA2999910.5137703314010537No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATACTGTAGGCACCATCA2805550.48048386560337675No Hit
TTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATC2321540.39759138612851785No Hit
TTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCG2321050.3975074677901722No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGAT2292810.3926710313108226No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGGCTGTAGGCACCAT2237280.38316085717136494No Hit
TCGGTTGGCCTCGGATAGCCGGTCCTGTAGGCACCATCAATAGATCGGAA2177390.37290398108254585No Hit
AGCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAATAGATCGGA2116840.3625340721298327No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAA2093660.35856422094033824No Hit
CGGTTGGCCTCGGATAGCCGGTCCTGTAGGCACCATCAATAGATCGGAAG2021670.34623507568012646No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGA1949300.33384085089221804No Hit
TTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCAT1938340.3319638203039152No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT1766350.30250848354459003No Hit
CGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAATAGATCGGAAG1725220.2954644809810047No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA1719160.2944266337761584No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG1699030.29097913142738685No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGA1698120.290823283084745No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCTGTAGGCACCATCAATAGATCGGAA1679870.28769775313615675No Hit
TTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGA1667580.28559294420091574No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA1579400.2704910685369975No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACC1490630.2552881483432345No Hit
CCGGGGTCCGGTGCGGAGTGCCCTTCTGTAGGCACCATCAATAGATCGGA1377050.23583622003854146No Hit
GGTTGGCCTCGGATAGCCGGTCCTGTAGGCACCATCAATAGATCGGAAGA1346940.23067952377815842No Hit
CGCTTCTGGCGCCAAGCGCCCGGCTGTAGGCACCATCAATAGATCGGAAG1342490.22991740825644785No Hit
GTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATC1315010.22521113083249147No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG1275640.21846854923929054No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAA1040790.17824768850362266No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG1029490.17631242886422285No Hit
TAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGGCTGTAGGCACCA977460.1674016714272341No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCTGTAGGCACCATCAATAGATCGGA973610.16674231305451825No Hit
CTCGGTTGGCCTCGGATAGCCGGTCCTGTAGGCACCATCAATAGATCGGA973530.16672861210131895No Hit
CCGGGGTCCGGTGCGGAGTGCCCTCTGTAGGCACCATCAATAGATCGGAA967310.1656633629900741No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACATCAAT962560.1648498688938662No Hit
TCGGTTGGCCTCGGGTAGCCGGTCCTGTAGGCACCATCAATAGATCGGAA911040.156026455033523No Hit
CACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAAGAG854160.14628507730882725No Hit
TTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA847450.14513590985923674Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCATC816750.13987816906900893No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGTACCATCAA811540.1389858944919051No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAATA784870.13441833921909158No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAGA782790.13406211443591No Hit
GCGAGCGCACGGGGTCGGCGGCGACCTGTAGGCACCATCAATAGATCGGA777860.13321779319500374No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACATCA768120.13154970214299008No Hit
CGCTTCTGGCGCCAAGCGCCCGGCCTGTAGGCACCATCAATAGATCGGAA757140.1296692463163874No Hit
CGGTTGGCCTCGGGTAGCCGGTCCTGTAGGCACCATCAATAGATCGGAAG740950.12689651591268095No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACTATCAA729770.12498180770308008No Hit
TTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAAG725860.12431217361546472No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAAT724520.12408268264937662No Hit
TTCGGTTGGCCTCGGATAGCCGGTCCTGTAGGCACCATCAATAGATCGGA715780.1225858535123541No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA713790.12224504230152174No Hit
TCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAAGA705040.12074650054534931No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGTACCATC673790.11539456570187638No Hit
GGTTGGCCTCGGGTAGCCGGTCCTGTAGGCACCATCAATAGATCGGAAGA669620.11468040351636334No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACTATC640410.1096778429794723No Hit
TTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCTGTAGGCACCATC631170.10809538288495424No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA623860.10684345828636903No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCATCATCAA617310.10572169274317711No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACACTGTAGGCACCATCAA598070.10242661349874767No Hit
ACTGACCCGGTGAGGCGGGGCTGTAGGCACATCAATAGATCGGAAGAGCA589080.1008869688829774No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)