FastQCFastQC Report
Sat 2 Sept 2023
SRR12494969_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12494969_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56205243
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length18-75
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCCAGCGGTGCCTCCA2152650.38299807724343443No Hit
ATCCCACCGCTGCCACCA1931850.34371348594649787No Hit
ATCCCGGACGAGCCCCCA1450440.25806133424243No Hit
GTCTAGGGGTATGATTCTCGC1292960.23004259584822007No Hit
ATCCTGCCGACTACGCCA1121480.1995329866290232No Hit
AACCATCCTCTGCTACCA1093110.19448541482153187No Hit
TTCCCGGCCCATGCACCA1035540.18424259815049637No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGC1028380.18296869564286022No Hit
GTCTAGGGGTATGATTCTCGCTT974640.1734073100618033No Hit
GTATAGTGGTGAGTATCCCCGCCTGTCAC864530.15381661102328123No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCC823050.14643651660753426No Hit
ACCCCGTCCGTGCCTCCA805560.14332470726974705No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGC798540.14207571347036074No Hit
GTATAGTGGTGAGTATCCCCGCCTGTCACG790820.14070217612972513No Hit
TCCCCGGCATCTCCACCA787700.14014706777444233No Hit
ATCCCACTTCTGACACCA783970.13948342861892796No Hit
GTATAGTGGTGAGTATCCCCGCC745680.13267089691258874No Hit
AACCGGGCGGAAACACCA733810.13055899429168913No Hit
TTCGATTCCCCGACGGGGAGCCA719290.1279756054074884No Hit
GTCTAGGGGTATGATTCTCG711950.1266696774178167No Hit
GTATAGTGGTGAGTATCCCCGCCTGTCACGCG703010.1250790784767179No Hit
ATCCCACTCCTGACACCA691960.12311306971842467No Hit
AGACCGGGGTTCGATTCCCCGACGGGGAGCCA673300.11979309474740639No Hit
CTCAGTCGGTAGAGCATCAGA671430.119460385572926No Hit
GTCTAGGGGTATGATTCTC654730.11648913251740588No Hit
ATCTCGGTGGAACCTCCA636950.11332572656967252No Hit
GTATAGTGGTGAGTATCCCCGC599510.10666442630627893No Hit
GTATAGTGGTGAGTATCCCC594140.10570899942555181No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTC592630.10544034121514252No Hit
ATCCCATCCTCGTCGCCA582390.10361844712600922No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)