FastQCFastQC Report
Sat 2 Sept 2023
SRR12494970_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12494970_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36434357
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length18-75
%GC61

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGG26665447.318762342917154No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGG7573742.0787357383581657No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAG6473501.7767570318312467No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAAT5813821.5956971602380687No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGGG5132281.408637457222039No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC5125551.4067902996064952No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGG5053771.3870891148154474No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGG4568611.253929086768294No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGG3072030.8431684412599899No Hit
GAGGGCGCACCACCGGCCCGTCTCGC2859720.7848965195131616No Hit
TCGCTTCTGGCGCCAAGCGCCCGGC2835370.7782132672191799No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGA2469670.6778409730134664No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAC2389060.6557162515589338No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGC2166990.5947655395702469No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGG2151040.5903878034680289No Hit
TTTTCACTGACCCGGTGAGGCGGGG2010550.5518280451607805No Hit
CGAGGGCGCACCACCGGCCCGTCTCGC1981730.5439179288933245No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAA1962860.5387387514482552No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGC1901770.5219716104774402No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGG1733110.4756801389413844No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT1725960.47371770551625214No Hit
ATTGATCATCGACACTTCGAACGCAC1647130.4520815339214028No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGA1510270.4145180879684524No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGA1490220.409015040391683No Hit
TAAGGCCTACTTCACAAAGCGCCTTC1470700.4036574599079654No Hit
CACTCGCCGAATCCCGGGGCCGAGGGA1411960.38753531453841766No Hit
GACCTGGTATTGCAGTACCTCCAGG1391550.38193345912485843No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG1385690.38032508711488994No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCG1372220.3766280272216688No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT1350880.37077091822973574No Hit
TGCTAAGACTTCACCAGTCAAAGCGA1301480.3572122872924586No Hit
TTGATCATCGACACTTCGAACGCAC1186860.32575296992341596No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTT1171990.3216716573315676No Hit
ATTGATCATCGACACTTCGAACGCA1157090.31758211075332No Hit
TTTCACTGACCCGGTGAGGCGGGG1144900.31423636761312956No Hit
TTCACTGACCCGGTGAGGCGGGG1075080.2950731366001601No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGC1063220.2918179673103604No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGG1055280.28963870557671706No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAT999480.2743234908742866No Hit
AACGATGCCGACCGGCGATGCGGCGGCG987190.27095030111276563No Hit
ACCTCTAGCATCACCAGTATTAGAGGC972320.2668689885209172No Hit
AAACCTGCATTAAAAATTTCGGTTGGG934590.2565133782929118No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAA923890.25357658981054615No Hit
CCGCCACGCAGTTTTATCCGGTAAAGC910420.24987952991732504No Hit
GCACCACCGGCCCGTCTCGC878950.2412420781846102No Hit
CGCCACGCAGTTTTATCCGGTAAAGC827800.22720313137404896No Hit
ATGCTCATAAGGAAAGGTTAAAAA827090.22700826036260224No Hit
TACCTGGTTGATCCTGCCAGTAGCAT766670.21042501175470174No Hit
ATTGATCATCGACACTTCGAACGC761450.2089922981212486No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAG757100.20779837009337093No Hit
CGACTGCATAATTTGTGGTAGTGGGG752880.20664012267322296No Hit
TAAGGCCTACTTCACAAAGCGCCTTCC710840.19510156306587215No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGA690800.18960126015123582No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGA689810.18932953860006368No Hit
TCGCTTCTGGCGCCAAGCGCCCGG674080.1850121850647728No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC667480.18320070805695843No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAG663590.1821330344872012No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT648300.1779364460857646No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAG623790.17120927919765402No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGA609760.167358518224982No Hit
TTTGGTGACTCTAGATAACCTCGG607310.16668607600238422No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAA601250.16502281074975467No Hit
ACAGGGAAGAGCCCAGCGCCGAATCCCC582800.15995890911427366No Hit
CCTCTAGCATCACCAGTATTAGAGGC564010.15480168896626886No Hit
TTGATCATCGACACTTCGAACGCA561580.1541347360679372No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAA518770.14238483747634137No Hit
CAGGATAGCTGGCGCTCTCGCAGA509500.1398405356790021No Hit
AACCTATTCTCAAACTTTAAATGGGT508340.13952215487156805No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT502920.13803454799545387No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGC501600.137672252593891No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGC499740.1371617454371433No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTT493490.13544633160398578No Hit
ACGATCAAAAGGGACAAGCATCAAGCACGCAGC448730.12316122389644478No Hit
TTTGGTGACTCTAGATAACCTCGGG444830.12209080566455448No Hit
ACGATGCCGACCGGCGATGCGGCGGCG442220.1213744488478279No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCG439430.12060868811270638No Hit
TTCTTGGACCGGCGCAAGACGGACCA435310.11947788731388892No Hit
AGCCTGGTGATAGCTGGTTGTCCAAGATAGAAT425850.11688143693602167No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGA417560.11460611202772153No Hit
CACTCGCCGAATCCCGGGGCCGAGGG412190.11313222846227257No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGG409930.11251193482020282No Hit
GCCGCGACCGGCTCCGGGACGGCTGGG390900.10728884278100476No Hit
TTGGTGACTCTAGATAACCTCGGG383750.10532640935587254No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)