FastQCFastQC Report
Sat 2 Sept 2023
SRR12494971_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12494971_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53086080
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length18-75
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAGGGGTATGATTCTCGC1116800.21037529989029138No Hit
GTCTAGGGGTATGATTCTCGCTT951580.17925226349355614No Hit
ATCCCAGCGGTGCCTCCA863440.1626490409538621No Hit
CTCAGTCGGTAGAGCATCAGA798180.15035579948641903No Hit
GTATAGTGGTGAGTATCCCCGCCTGTCACG670400.1262854593897308No Hit
ATCCCACCGCTGCCACCA663760.12503466068694466No Hit
GTATAGTGGTGAGTATCCCCGCCTGTCAC652940.12299646159595887No Hit
GTCTAGCGGTTAGGATTCCTGGTTTTCACCC604110.11379819342471698No Hit
GTATAGTGGTGAGTATCCCCGCCTGTCACGCG598980.11283183840283555No Hit
ACGCAAATTCGTGAAGCGTTCCATATTTTT589690.11108185045872665No Hit
GTCTAGGGGTATGATTCTCG570890.10754043244481416No Hit
GTGCTCGCTTCGGCAGCACATATACTAAAATTGGAAC560060.10550034962084223No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)