FastQCFastQC Report
Sat 2 Sept 2023
SRR12494973_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12494973_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38710131
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length18-75
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGG15437183.9878914385487354No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC6325121.6339701872876637No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAT5067111.3089880785988557No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC4987781.2884947353962715No Hit
GACCTGGTATTGCAGTACCTCCAGG4682231.2095619102916495No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAAT4235161.0940701802326631No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGGG3878151.0018436775633748No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAC3720010.9609913229175071No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGG3307900.8545308203684456No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT3178080.8209943799983523No Hit
CGACTGCATAATTTGTGGTAGTGGGG3163370.8171943411919738No Hit
AGCTAGGAATAATGGAAT3046000.7868741131359127No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGG2888550.7462000064014249No Hit
AGTCCGCCCGGAGGATTC2824220.7295816177940602No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAG2722370.7032706760925196No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGG2317890.5987812337808931No Hit
TCGCTTCTGGCGCCAAGCGCCCGGC2044500.528156311328422No Hit
TGCTAAGACTTCACCAGTCAAAGCGA1892680.4889366042186734No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGA1886300.4872884568641734No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGC1806560.4666891982359863No Hit
ATTGATCATCGACACTTCGAACGCAC1724540.4455009465093259No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGG1668140.43093111723130045No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGC1624390.4196291663285769No Hit
CACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCT1607160.41517813515020136TruSeq Adapter, Index 10 (100% over 50bp)
TCGACTGCATAATTTGTGGTAGTGGGG1547360.39972998283059286No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGG1464290.3782704842822671No Hit
AACGATGCCGACCGGCGATGCGGCGGCG1368440.3535095244188143No Hit
AGCCTGGTGATAGCTGGTTGTCCAAGATAGAAT1333370.34444988057519105No Hit
ATGCTCATAAGGAAAGGTTAAAAA1317230.34028042943073483No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG1278310.3302262139076719No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT1276770.32982838523589597No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGG1272580.32874598125229804No Hit
TTGATCATCGACACTTCGAACGCAC1232390.31836368624017314No Hit
GAGGGCGCACCACCGGCCCGTCTCGC1222670.31585271566247086No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTT1185100.3061472460529777No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGC1169090.30201137784834675No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGA1145150.29582695031437634No Hit
CGAGGGCGCACCACCGGCCCGTCTCGC1134260.293013733278247No Hit
CTCGCTTCTGGCGCCAAGCGCCCGG1133580.2928380686699304No Hit
ACCTCTAGCATCACCAGTATTAGAGGC1117460.2886737841316011No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAA1109470.2866097249838808No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGA1102560.2848246625670164No Hit
GCACCACCGGCCCGTCTCGC1098700.2838275075845132No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGG1090820.28179186477049123No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAA909910.2350573290490802No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGC909330.23490749747139836No Hit
AACCTCGGGCCGATCGCAC905280.23386125973068908No Hit
ACTGACCCGGTGAGGCGGGG898380.2320787806168881No Hit
TAAGGCCTACTTCACAAAGCGCCTTC849640.21948776148548815No Hit
TTTTCACTGACCCGGTGAGGCGGGG838340.2165686290237561No Hit
CCTCTAGCATCACCAGTATTAGAGGC835950.21595121959158445No Hit
TAAGGCCTACTTCACAAAGCGCCTTCC819710.2117559354164934No Hit
ATTGATCATCGACACTTCGAACGCA803190.20748831875562496No Hit
ATCGACACTTCGAACGCAC713880.1844168390956879No Hit
AGATAACCTCGGGCCGATCGCAC705480.18224686452236494No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGA704930.18210478285387358No Hit
ACGATCAAAAGGGACAAGCATCAAGCACGCAGC703710.18178961988012907No Hit
GACCTGGTATTGCAGTACCTCCAG680330.17574985731771353No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAA655760.16940268169074396No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAA645710.16680646211194688No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCG618500.15977729447621863No Hit
TTCACTGACCCGGTGAGGCGGGG596140.15400102882627806No Hit
AGCCTGGTGATAGCTGGTTGTCCAAGATAGAA584310.1509449813021816No Hit
TTTCACTGACCCGGTGAGGCGGGG583750.15080031633062674No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGA573370.1481188477507348No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGA564320.1457809584782857No Hit
CACTCGCCGAATCCCGGGGCCGAGGGA547100.1413325106029737No Hit
GACTGCATAATTTGTGGTAGTGGGG523030.13511450012917808No Hit
GACCTGGTATTGCAGTACTTCCAGG513530.13266036221887237No Hit
TCGCTTCTGGCGCCAAGCGCCCGG512730.132453697973794No Hit
CATGCTCATAAGGAAAGGTTAAAAA507730.13116204644205415No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAG498230.12870790853174843No Hit
AGTTGGTCCGAGTGTTGTGGGTTATTGTTA450440.11636230319137904No Hit
AAACATCACCTCTAGCATCACCAGTATTAGAGGC448490.11585855909400047No Hit
AGCCTGGTGATAGCTGGTTGTCCAAGATAGA447130.11550722987736725No Hit
CGCCACGCAGTTTTATCCGGTAAAGC432830.11181310649659129No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGC424790.10973613083355362No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT424580.10968188146922055No Hit
CGATCAAAAGGGACAAGCATCAAGCACGCAGC422260.10908255515849326No Hit
AAACCTGCATTAAAAATTTCGGTTGGG419200.10829206442106848No Hit
CTCTAGCATCACCAGTATTAGAGGC411790.10637783685103003No Hit
CCGCCACGCAGTTTTATCCGGTAAAGC406590.1050345192580206No Hit
CGCTTCTGGCGCCAAGCGCCCGG395420.10214896973611377No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)