FastQCFastQC Report
Sat 2 Sept 2023
SRR12494974_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12494974_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29098608
Total Bases819.4 Mbp
Sequences flagged as poor quality0
Sequence length18-75
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGG17060935.863143006703276No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC5924882.0361386359100067No Hit
TCGACTGCATAATTTGTGGTAGTGGGG4323491.4858064688180275No Hit
GCACCACCGGCCCGTCTCGC3484601.197514327833139No Hit
CGACTGCATAATTTGTGGTAGTGGGG3141991.0797733004960237No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCG3096861.0642639675409902No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGG2991341.028001064518275No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAG2902550.9974875774126377No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGGG2596680.8923725835957513No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGC2372360.8152829853579251No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGG2071580.7119172161087568No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGG1926480.6620522878620173No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGG1900990.6532924186614012No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG1771070.6086442348032594No Hit
ATTGATCATCGACACTTCGAACGCAC1716460.5898770140482321No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGC1284290.44135788213649263No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGC1257220.43205503163587755No Hit
AACGATGCCGACCGGCGATGCGGCGGCG1186500.40775146357516484No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT1177740.40474101029162635No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTT1159120.3983420787688538No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGA1150760.39546908910556816No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGA1134820.38999116383849014No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGG1098260.3774269889473751No Hit
TTGATCATCGACACTTCGAACGCAC1082530.37202123208092974No Hit
GACCTGGTATTGCAGTACCTCCAGG1000210.34373121903288295No Hit
TGCTAAGACTTCACCAGTCAAAGCGA949170.3261908610886129No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT930640.3198228588803973No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGA923370.3173244575823009No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGC900410.3094340457797844No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTT870460.2991414572133485No Hit
GACTGCATAATTTGTGGTAGTGGGG789300.27125008866403505No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCG751830.2583731840368446No Hit
ACCTCTAGCATCACCAGTATTAGAGGC672840.2312275556274032No Hit
TCGCTTCTGGCGCCAAGCGCCCGG652130.2241103766888093No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCG652120.22410694009830298No Hit
ACTGACCCGGTGAGGCGGGG636430.21871492959388297No Hit
TTCACTGACCCGGTGAGGCGGGG618080.21240878601478116No Hit
CACCACCGGCCCGTCTCGC590010.20276227646353392No Hit
ATTGATCATCGACACTTCGAACGCA588280.20216774630594014No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGA586120.20142544275657445No Hit
TTTTCACTGACCCGGTGAGGCGGGG580600.19952844479708443No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGC559530.19228754860026293No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGA558830.19204698726482036No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCG553220.19011905999077344No Hit
AACTTTGCAAGGAGAGCCAAAGCTAAGAC550780.18928053190723074No Hit
GAGGGCGCACCACCGGCCCGTCTCGC546550.18782685412305633No Hit
ACGATTAAAGTCCTACGTGATCTGAGT537090.18457583950407525No Hit
AAACCTGCATTAAAAATTTCGGTTGGG534520.18369263574395037No Hit
AAACGATGCCGACCGGCGATGCGGCGGCG529080.18182313050851093No Hit
TTTCACTGACCCGGTGAGGCGGGG513650.17652047135725532No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAG504210.17327632991928688No Hit
ACTTTGCAAGGAGAGCCAAAGCTAAGAC469210.16124826314715812No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGG467610.16069840866614649No Hit
GCACCACCGGCCCGTCTCG466260.16023446894779297No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAG463590.15931689928260487No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAG456150.1567600759459009No Hit
CTGGTCCGATGGTAGTGGGTTATCAGAA436340.14995219015287606No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT429120.1474709718073112No Hit
ACTAAAATTGGAACGATACAGAGAA417660.14353263908706562No Hit
AACTTTGCAAGGAGAGCCAAAGCTAAGACC417400.14344328773390122No Hit
AAGGGACAAGCATCAAGCACGCAGC416400.14309962868326898No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGC391780.134638742856703No Hit
AGTTGGTCCGAGTGTTGTGGGTTATTGTTA382480.13144271368582305No Hit
AGGGCTTCTGTCGTGAGTGGCACACGTAGG379140.13029489245671133No Hit
AGCCTGGTGATAGCTGGTTGTCCAAGATAGA376440.12936701302000425No Hit
ATGCTCATAAGGAAAGGTTAAAAA376040.12922954939975137No Hit
CTGGTCCGAAGGTAGTGAGTTATCTCAA351130.120669002448502No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGC344880.11852113338205045No Hit
TTGATCATCGACACTTCGAACGCA332790.11436629545990654No Hit
TCGACTGCATAATTTGTGGTAGTGGG320700.11021145753776264No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG318320.10939354899725787No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGA315180.10831445957827261No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT311870.10717694812067985No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCG310970.10686765497511083No Hit
TCGCTTCTGGCGCCAAGCGCCCGGC302370.10391218713967348No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAG295490.1015478128713236No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)