FastQCFastQC Report
Sat 2 Sept 2023
SRR12494975_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12494975_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51146338
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length18-75
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGTGGTGAGTATCCCCGCCTGTCAC786330.153741212127445No Hit
ATGACACGCAAATTCGTGAAGCGTTCCATATTTTT767260.1500126949460194No Hit
GTCTAGCGGTTAGGATTCCTGGTTTTCACCC752050.14703887500215557No Hit
GTATAGTGGTGAGTATCCCCGCCTGTCACG748470.1463389226419299No Hit
GTGCTCGCTTCGGCAGCACATATACTAAAATTGGAAC728090.14235427764153907No Hit
ACGCAAATTCGTGAAGCGTTCCATATTTTT706670.13816629452532847No Hit
TCCTCACACGGGGCACAATAATTT688880.13468803964029644No Hit
CTGGGCCCATAACCCAGAGGTCGATGGATC671270.13124497789069475No Hit
GTATAGTGGTGAGTATCCCCGCCTGTCACGCG649670.1270218016390538No Hit
GTCTAGCGGTTAGGATTCCTGGTTTTCAC633450.12385050910194197No Hit
AGACCGGGGTTCGATTCCCCGACGGGGAGCCA614650.12017478162366188No Hit
GTCTAGCGGTTAGGATTCCTGGTTTTCACC611440.11954717070848749No Hit
GTGCTCGCTTCGGCAGCACATATACTAAAA585350.11444612124527859No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)