FastQCFastQC Report
Sat 2 Sept 2023
SRR12494976_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12494976_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38280210
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length18-75
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCAAATTCGTGAAGCGTTCCATATTTTT1605370.4193733524450362No Hit
ATCCCAGCGGTGCCTCCA798940.20870836393008294No Hit
ATGACACGCAAATTCGTGAAGCGTTCCATATTTTT743020.19410029359818037No Hit
GTATAGTGGTGAGTATCCCCGCCTGTCACG721030.18835581100521653No Hit
GTATAGTGGTGAGTATCCCCGCCTGTCACGCG626970.1637843679540943No Hit
AAACAGGAGATCCTGGGTTCGAATCCCAGCGGTGCCTCCA535980.14001490587434082No Hit
TCCTCACACGGGGCACAATAATTT480170.12543557101698238No Hit
TCCTGGGTTCGAATCCCAGCGGTGCCTCCA474860.12404843129125989No Hit
AAATTCGTGAAGCGTTCCATATTTTT468270.1223269151344781No Hit
AGACCGGGGTTCGATTCCCCGACGGGGAGCCA433410.11322038201984785No Hit
AATTCGTGAAGCGTTCCATATTTTT425290.11109918153531549No Hit
ATAGCTCAGTCGGTAGAGCATCAGA416990.10893095936516545No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)