FastQCFastQC Report
Sat 2 Sept 2023
SRR12494977_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12494977_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31110733
Total Bases896 Mbp
Sequences flagged as poor quality0
Sequence length18-75
%GC64

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGG21486876.906577868158877No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC7208552.317062089151033No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGG4864461.5635954318401948No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGG3397781.0921568450347987No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAG3346721.075744502709081No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGGG3102760.9973278353807994No Hit
GACCTGGTATTGCAGTACCTCCAGG2557840.82217284947931No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGG2381010.7653339443979028No Hit
AACAGGGAAGAGCCCAGCGCCGAATCCCCG2287200.7351803636384909No Hit
AGTCCGCCCGGAGGATTC2175740.6993534996427118No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGG2108600.6777725230710572No Hit
CGACTGCATAATTTGTGGTAGTGGGG1963250.6310523123964967No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG1930730.6205993282125497No Hit
ACAGGGAAGAGCCCAGCGCCGAATCCCC1845730.5932775675841517No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGG1594030.5123730128762958No Hit
GAGGGCGCACCACCGGCCCGTCTCGC1483540.4768579383841583No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGG1435650.46146453701364093No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGG1425410.45817306843911393No Hit
CACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGTCTTCTGCT1402320.4507511925225291TruSeq Adapter, Index 11 (100% over 50bp)
AACGATGCCGACCGGCGATGCGGCGGCG1373790.44158072392572684No Hit
TCGACTGCATAATTTGTGGTAGTGGGG1371680.4409025013971867No Hit
TCGCTTCTGGCGCCAAGCGCCCGGC1266340.4070428041666521No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGA1197860.3850311080745028No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGA1194080.38381609330773403No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGC1183050.3802706930756019No Hit
AGGGAAGAGCCCAGCGCCGAATCCCC1167900.3754009910341875No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGA1153350.3707241484795617No Hit
ACCTCTAGCATCACCAGTATTAGAGGC1131610.36373620640825144No Hit
AACAGGGAAGAGCCCAGCGCCGAATCCCC1101940.3541993047865507No Hit
TGCTAAGACTTCACCAGTCAAAGCGA1031210.33146438561894376No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGC1001300.3218503402025275No Hit
ATGCTAAGACTTCACCAGTCAAAGCGA960650.3087841099725937No Hit
AAGCTTGGGCTGCAGGTCGACCCGTGC927160.29801933628500493No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAA868700.2792283936222268No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT847940.27255545537933806No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGC837740.2692768441039303No Hit
CGAGGGCGCACCACCGGCCCGTCTCGC834600.26826754612306947No Hit
CACTCGCCGAATCCCGGGGCCGAGGGA827660.2660368047258803No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAA827540.26599823282852253No Hit
ACAGGGAAGAGCCCAGCGCCGAATCCCCG827060.2658439452390916No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGA785780.2525752125480296No Hit
TAAGGCCTACTTCACAAAGCGCCTTCC780040.25073019012441783No Hit
GACCTGGTATTGCAGTACCTCCAG772520.24831301788999954No Hit
GCACCACCGGCCCGTCTCGC771520.24799158541201843No Hit
AGCCTGGTGATAGCTGGTTGTCCAAGATAGAAT702180.22570345738880532No Hit
AGCGGAGGAAAAGAAACTAACC663980.21342473672992532No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGC660940.21244758199686262No Hit
AACAGGGAAGAGCCCAGCGCCGAATCCCCGC660050.2121615070914594No Hit
ACAGGGAAGAGCCCAGCGCCGAATCCCCGCCC655360.2106539887697278No Hit
CAGGGAAGAGCCCAGCGCCGAATCCCC643710.20690930040124736No Hit
TAAGGCCTACTTCACAAAGCGCCTTC641550.20621500624880806No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCG629020.20218745729970425No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAC628880.2021424567527869No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGG627050.2015542353180814No Hit
CTCGCTTCTGGCGCCAAGCGCCCGG614790.19761347313803243No Hit
CAGCGGAGGAAAAGAAACTAACC584470.18786764040564394No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAT538880.1732135337344832No Hit
GCGACCGGCTCCGGGACGGCTGGG522610.16798382731772987No Hit
AACAGGGAAGAGCCCAGCGCCGAATCCCCGCCC502310.16145874801471247No Hit
GCAGTCCGCCCGGAGGATTCAACCCGGCGGCGGG488500.15701976549379276No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCC484010.1555765336676574No Hit
CCTCTAGCATCACCAGTATTAGAGGC480470.1544386626956041No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC479770.1542136599610173No Hit
AGGGAAGAGCCCAGCGCCGAATCCCCGCCC477870.15360293825285312No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT477250.1534036501165048No Hit
GAGAAATAAGGCCTACTTCAC475420.15281542868179931No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGA472820.1519797042390483No Hit
GACTGCATAATTTGTGGTAGTGGGG468050.1504464713190782No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAG461270.148267159118366No Hit
AGTTGGTCCGAGTGTTGTGGGTTATTGTTA457340.14700392947990007No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAG454300.14602677474683737No Hit
TCGCTTCTGGCGCCAAGCGCCCGG447450.1438249622726665No Hit
AACCCGGCGGCGGGTCCGGCCGTGTCGG447110.14371567523015288No Hit
ACCCCTCCCACGGCGCGACCGC435560.14000313010947057No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGG434840.13977169872532413No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGG427940.1375538146272542No Hit
GCAGTCCGCCCGGAGGATTC423700.13619094092061412No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGA416470.13386698410481038No Hit
ACAGGGAAGAGCCCAGCGCCGAATCCCCGC407950.13112837939241098No Hit
ATGCTCATAAGGAAAGGTTAAAAA407370.1309419485551819No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGC406620.13070087419669604No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTC406460.13064944500021905No Hit
AGAAATAAGGCCTACTTCAC405680.13039872766739377No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAAT404920.13015443898412807No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGT400970.1288847806961025No Hit
CTGCATAATTTGTGGTAGTGGGG397400.1277372667497098No Hit
CACTCGCCGAATCCCGGGGCCGAGGG393380.1264451081882256No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGC392840.12627153465011576No Hit
ATGCTAAGACTTCACCAGTCAAAGCGAA389450.12518187854975968No Hit
AACAGGGAAGAGCCCAGCGCCGAATCCCCGCCCC388600.12490866094347568No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCC379080.1218486237530951No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTT374350.12032824813224426No Hit
AGGGAAGAGCCCAGCGCCGAATCCCCGC367550.11814250728197243No Hit
TGGCAATTTTTGACAGTCTCTACGGAGACTG360650.11592462318390248No Hit
ACGATGCCGACCGGCGATGCGGCGGCG357380.1148735389809041No Hit
TTTTTGACAGTCTCTACGGAGACTG352820.11340780688131005No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCC333540.10721058870583344No Hit
ACGATCAAAAGGGACAAGCATCAAGCACGCAGC332440.10685701298005419No Hit
TTTGACAGTCTCTACGGAGACTG332330.10682165540747625No Hit
TCAGCGGAGGAAAAGAAACTAACC323750.10406376474639796No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAG323430.103960906353444No Hit
AGTTGGTCCGAGTGTTGTGGGTT314940.10123194461538403No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTT314120.10096836998343948No Hit
ACAGGGAAGAGCCCAGCGCCGAATCCCCGCCCC313260.10069193805237568No Hit
GAAATAAGGCCTACTTCAC313110.10064372318067852No Hit
TTGACAGTCTCTACGGAGACTG311520.10013264554068849No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)