FastQCFastQC Report
Sat 2 Sept 2023
SRR12494978_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12494978_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32379613
Total Bases880.5 Mbp
Sequences flagged as poor quality0
Sequence length18-75
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGG13614034.204506706117828No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAG6366801.966298979546173No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGG5911121.8255684525939206No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGG4026901.2436529120962625No Hit
GCACCACCGGCCCGTCTCGC3975101.2276551915552543No Hit
ACGATTAAAGTCCTACGTGATCTGAGT3969621.2259627686099892No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCG3511961.0846207457760537No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGG3374031.0420229543818205No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGC3240121.0006666849291868No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC2752430.8500503078897206No Hit
AGTCCGCCCGGAGGATTC2423530.7484740475434343No Hit
ATTGATCATCGACACTTCGAACGCAC2345730.7244465831015336No Hit
TTGATCATCGACACTTCGAACGCAC2218830.6852552561391021No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGGG2201400.6798722393624655No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGG2149070.6637108355804006No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGC1761420.5439904423811365No Hit
AAGGGACAAGCATCAAGCACGCAGC1535770.47430153041050865No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTT1532520.47329781242289704No Hit
ATGCTCATAAGGAAAGGTTAAAAA1409620.4353418306759874No Hit
AAACCTGCATTAAAAATTTCGGTTGGG1383780.427361500583716No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGG1356820.41903527383109856No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAG1343980.41506981568927337No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGA1343440.4149030440851779No Hit
CGACTGCATAATTTGTGGTAGTGGGG1334430.41212042898721485No Hit
ACCTCTAGCATCACCAGTATTAGAGGC1281230.3956903376207739No Hit
GCACCACCGGCCCGTCTCG1253950.3872652832509147No Hit
ATTGATCATCGACACTTCGAACGCA1237910.38231154893667196No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGA1222700.37761414875465005No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGA1200020.370609741382641No Hit
AACGATGCCGACCGGCGATGCGGCGGCG1196450.36950719577778773No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG1120800.3461437293892302No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGC1067120.3295653965969266No Hit
CACCACCGGCCCGTCTCGC991520.30621737202356314No Hit
ACTGACCCGGTGAGGCGGGG965030.29803629833376943No Hit
ACGATTAAAGTCCTACGTGATCTGAGTT953960.2946174804498127No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAG952790.2942561419742725No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGG943770.29147043851327065No Hit
ATCGACACTTCGAACGCAC936230.28914181278201195No Hit
CACTCGCCGAATCCCGGGGCCGAGGGA933390.2882647176789914No Hit
TCGACTGCATAATTTGTGGTAGTGGGG925850.2859360919477327No Hit
ACAGGGAAGAGCCCAGCGCCGAATCCCC913180.2820231359775671No Hit
TGCTAAGACTTCACCAGTCAAAGCGA852770.263366334860148No Hit
AGGGACAAGCATCAAGCACGCAGC790350.24408877277192909No Hit
GACCTGGTATTGCAGTACCTCCAGG786330.24284725083032957No Hit
GAGGGCGCACCACCGGCCCGTCTCGC781780.24144204564767344No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCG761990.23533017519387894No Hit
TTGATCATCGACACTTCGAACGCA761130.23506457597254174No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTT715500.2209723754264759No Hit
TAAGGCCTACTTCACAAAGCGCCTTCC709500.2191193576031931No Hit
TCGCTTCTGGCGCCAAGCGCCCGG704810.2176709153379937No Hit
CCTCTAGCATCACCAGTATTAGAGGC701830.21675058315242987No Hit
TTTTCACTGACCCGGTGAGGCGGGG700240.21625953342925994No Hit
TTCACTGACCCGGTGAGGCGGGG665680.20558615076715095No Hit
AAAGGGACAAGCATCAAGCACGCAGC659700.20373930966994572No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGC629090.19428583040816455No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAG623070.19242663585880412No Hit
ATGCTAAGACTTCACCAGTCAAAGCGA621210.19185220033358646No Hit
TTTCACTGACCCGGTGAGGCGGGG593010.1831430165641572No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGA553070.17080809458717125No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGC550680.17006997582089692No Hit
CTCTAGCATCACCAGTATTAGAGGC535480.16537566400191378No Hit
ACCACCGGCCCGTCTCGC534730.16514403677400344No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGC532990.16460666160525142No Hit
AAACGATGCCGACCGGCGATGCGGCGGCG525210.1622039151610614No Hit
TAAATTTGCCCACAGAAC503050.15536010266707015No Hit
ACTAAAATTGGAACGATACAGAGAA496800.15342987576781722No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCG494870.15283382170132792No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCG482640.1490567537048698No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT479700.1481487749714612No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGG479160.14798200336736575No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT472740.14599927429645315No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT472230.1458417677814741No Hit
AGTTGGTCCGAGTGTTGTGGGTTATTGTTA472190.1458294143293189No Hit
ACTTTGCAAGGAGAGCCAAAGCTAAGAC466480.14406595903416142No Hit
GACTGCATAATTTGTGGTAGTGGGG465230.14367991365431082No Hit
AACTTTGCAAGGAGAGCCAAAGCTAAGAC464270.14338343080258556No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGA457350.1412462835797327No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGG444630.13731788579437315No Hit
CTGCATAATTTGTGGTAGTGGGG433640.13392377481472678No Hit
AGCCTGGTGATAGCTGGTTGTCCAAGA397970.12290758385531043No Hit
CTCGCTTCTGGCGCCAAGCGCCCGG391400.12087852933881575No Hit
AAAAGGGACAAGCATCAAGCACGCAGC390350.12055425121974125No Hit
TCGCTTCTGGCGCCAAGCGCCCGGC377290.11652084909106233No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT370850.11453194329407211No Hit
AACAGGGAAGAGCCCAGCGCCGAATCCCC368600.11383706161034106No Hit
AGGTCGGTTTCTATCTACTTCAAATTCC368260.11373205726702168No Hit
AACTTTGCAAGGAGAGCCAAAGCTAAGACC362550.1119686019718642No Hit
CACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCTTCTGCT348210.10753988937421827TruSeq Adapter, Index 8 (100% over 50bp)
ATAAACGATGCCGACCGGCGATGCGGCGGCG330250.10199318935652504No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)