FastQCFastQC Report
Sat 2 Sept 2023
SRR12494979_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12494979_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51820147
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length18-75
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCCAGCGGTGCCTCCA6455341.2457201250316792No Hit
ATCCCACCGCTGCCACCA2538890.4899426472101671No Hit
ATCCCGGACGAGCCCCCA2431500.4692190471786968No Hit
TCCCCGTACGGGCCACCA2227070.4297691397903599No Hit
AACCATCCTCTGCTACCA2221080.428613218715879No Hit
ATCTCGGTGGAACCTCCA2141100.41317906720720027No Hit
ATCCTGCCGACTACGCCA1948370.37598696892928535No Hit
ACCCCGTCCGTGCCTCCA1871080.36107192054086606No Hit
CTCCTGGCTGGCTCGCCA1756820.339022581313789No Hit
TCCCCGGCATCTCCACCA1599180.3086019806157632No Hit
AACCGGGCGGAAACACCA1389550.2681486025116833No Hit
TTCCCGGCCAATGCACCA1239080.2391116335505571No Hit
ATCCCACTTCTGACACCA1226870.23675540711993737No Hit
GCCCACCCAGGGACGCCA1215110.23448601950125691No Hit
GTCCCTTCGTGGTCGCCA1214190.23430848237462545No Hit
ATCCCATCCTCGTCGCCA1174680.2266840346863547No Hit
TTCCGGCTCGAAGGACCA1159200.2236967795556427No Hit
TCCCCAGCATCTCCACCA1119470.21602987733709053No Hit
GTCTAGGGGTATGATTCTC1110930.21438186966162023No Hit
GCCCCACGTTGGGCGCCA1046990.20204303936073356No Hit
GCCCCAGTGGAACCACCA1043730.20141394041201774No Hit
TTCCCGGTCAGGGAACCA1039090.2005185357733547No Hit
ATCTCGGTGGGACCTCCA1026720.19813143332071212No Hit
ATCCCAGCGGGGCCTCCA973780.1879153295338973No Hit
TCCCGGGTTTCGGCACCA957870.18484509509399885No Hit
GTCTAGGGGTATGATTCTCGC941650.18171503836143113No Hit
ATCCGGGTGCCCCCTCCA885720.17092193891306406No Hit
TTCCCGGGCGGCGCACCA844410.16295013597703611No Hit
GTCTAGGGGTATGATTCTCG819620.15816628231486876No Hit
GTCCCTGTTCGGGCGCCA818130.15787874935978086No Hit
ATCCGAGTCACGGCACCA815110.15729596444409932No Hit
ATCCCACTCCTGACACCA803950.15514236190800462No Hit
TTCCCGGCCAACGCACCA755840.14585832803600499No Hit
TGCCCGCATCCTCCACCA708220.13666885198145037No Hit
CTCAGTCGGTAGAGCATCAGA678610.1309548581558443No Hit
GTCTAGGGGTATGATTCTCGCTT678310.1308969656145514No Hit
ACCCCACTCCTGGTACCA669730.1292412389335754No Hit
ACCCTGCTCGCTGCGCCA603300.11642190053995793No Hit
TCCCCGGCACCTCCACCA601610.11609577255734145No Hit
GTCCCATCTGGGTCGCCA601430.11606103703256573No Hit
GTGCTCGCTTCGGCAGCACATATACTAAAATTGGAAC595100.11483950441128622No Hit
ATGACACGCAAATTCGTGAAGCGTTCCATATTTTT588400.11354657098907883No Hit
CTCCCGGTATGGGAACCA547360.10562687134021446No Hit
ACGCAAATTCGTGAAGCGTTCCATATTTTT539900.10418727681339847No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)