FastQCFastQC Report
Sat 2 Sept 2023
SRR12494980_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12494980_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38765703
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length18-75
%GC59

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGG9362842.415238026252226No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAT8057502.0785125449679063No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAG5102831.3163259286178817No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGG4470461.153199775585135No Hit
CGACTGCATAATTTGTGGTAGTGGGG4272471.1021262789946051No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAAT3971861.0245809291785577No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC3772580.9731746642128482No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGA3474460.8962716347488914No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC3409430.8794964972001152No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGA3023720.7799987530214529No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGG2966430.7652202257237538No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAA2896320.7471346514727206No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGGG2824960.7287266272457383No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAA2565670.6618401838346644No Hit
GACCTGGTATTGCAGTACCTCCAGG2539670.655133224334923No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGG2418880.6239742382590096No Hit
CAGCGGAGGAAAAGAAACTAACC2188610.5645737934895698No Hit
CACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCT2099200.5415095916098827TruSeq Adapter, Index 12 (100% over 50bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGC2038960.5259700823689435No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAA2009410.5183473649375068No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGA1911190.49301053562733016No Hit
AGCGGAGGAAAAGAAACTAACC1855860.47873760989191916No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGC1823280.4703332737187818No Hit
AGCTAGGAATAATGGAAT1795820.46324969264713195No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGG1755090.4527429826308064No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGG1736260.44788559619310914No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAC1587850.4096017554486243No Hit
ACAGGGAAGAGCCCAGCGCCGAATCCCC1573750.40596451971991837No Hit
ATTGATCATCGACACTTCGAACGCAC1367910.352866037280428No Hit
TCGACTGCATAATTTGTGGTAGTGGGG1355190.34958478632517No Hit
AGTTGGTCCGAGTGTTGTGGGTTATTGTTA1330400.3431899584021474No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGA1259000.3247716157759347No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT1167810.3012482451304959No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCG1122070.2894491556105664No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT1090990.2814317594085679No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGA1070190.2760661918087749No Hit
CACTCGCCGAATCCCGGGGCCGAGGGA1061630.27385805437347543No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGG1029800.26564718818590755No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGC1023190.26394207271308867No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT1014040.26158173888914127No Hit
TCAGCGGAGGAAAAGAAACTAACC1012480.2611793213191568No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG1011410.26090330413974433No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAA968060.24972073897382951No Hit
AGTCCGCCCGGAGGATTC966650.24935701540095895No Hit
GAGGGCGCACCACCGGCCCGTCTCGC958710.24730881315373024No Hit
GACTGCATAATTTGTGGTAGTGGGG949770.24500265092574228No Hit
TTGATCATCGACACTTCGAACGCAC946040.24404046019751013No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGG904570.23334285979542277No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGG873960.22544670478438117No Hit
AACAGGGAAGAGCCCAGCGCCGAATCCCCG871160.2247244168382552No Hit
CGCCACGCAGTTTTATCCGGTAAAGC863820.22283099057948205No Hit
CAGCGGAGGAAAAGAAACT826910.21330968768965702No Hit
CAGGGAAGAGCCCAGCGCCGAATCCCC812940.20970598675844987No Hit
AGCCTGGTGATAGCTGGTTGTCCAAGATAGAAT811040.20921586279500723No Hit
AACAGGGAAGAGCCCAGCGCCGAATCCCC803430.20725278734142907No Hit
CAGCGGAGGAGAAGAAACTAACC746990.19269352602737527No Hit
GACCTGGTATTGCAGTACCTCCAG738840.19059115218418715No Hit
AGATAACCTCGGGCCGATCGCAC723440.1866185684804942No Hit
ACCTCTAGCATCACCAGTATTAGAGGC720810.1859401337310973No Hit
AGGGAAGAGCCCAGCGCCGAATCCCC679050.17516772493458974No Hit
AGCGGAGGAAAAGAAACTAACCAGGATTCCCT673020.17361222625061126No Hit
CGAGGGCGCACCACCGGCCCGTCTCGC646860.16686399315394848No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGA640630.16525690247381816No Hit
AGCGGAGGAAAAGAAACT629870.1624812530808483No Hit
AGCGGAGGAGAAGAAACTAACC619400.15978041208229862No Hit
ACGATCAAAAGGGACAAGCATCAAGCACGCAGC611160.15765482184084215No Hit
GCACCACCGGCCCGTCTCGC593370.15306571378313455No Hit
TGCTAAGACTTCACCAGTCAAAGCGA592870.1529367337927549No Hit
CAGCGGAGGAAAAGAAACTAACCAGGATTCCCT579590.14951102524827165No Hit
CCTCTAGCATCACCAGTATTAGAGGC576780.14878615770233808No Hit
CCGCCACGCAGTTTTATCCGGTAAAGC547580.14125372626416707No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAG532990.13749009014488917No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTT527530.13608162864994347No Hit
GCGACCGGCTCCGGGACGGCTGGG521340.13448485636904353No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAG510650.13172726417472683No Hit
AACCTCGGGCCGATCGCAC507300.1308630982391832No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAT504770.13021045948786225No Hit
AGCGGAGGAAAAGAAACTAACCAGGATTCCCTC503520.12988800951191315No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGA497630.12836862522524098No Hit
GGAATGCAGCCCAAAGCGGGTGGTA496120.1279791056542945No Hit
TCGCTTCTGGCGCCAAGCGCCCGGC495790.1278939788606439No Hit
AGCCTGGTGATAGCTGGTTGTCCAAGATAGAA489740.12633332097705025No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGG484480.12497645147825645No Hit
AGTTGGTCCGAGTGTTGTGGGTT479700.12374340277022708No Hit
CGGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGG470000.12124119095686206No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCG466970.12045957221516143No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTT462600.11933228709924337No Hit
GACCTGGTATTGCAGTACTTCCAGG461050.11893244912906649No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAT458480.11826949197851512No Hit
GACCGGCTCCGGGACGGCTGGG448890.11579565576303363No Hit
CACTCGCCGAATCCCGGGGCCGAGGG444650.11470190544461427No Hit
CGATCAAAAGGGACAAGCATCAAGCACGCAGC444640.11469932584480667No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGC444610.1146915870453839No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAG425650.10980066581018794No Hit
ATTGATCATCGACACTTCGAACGCA422560.10900356946964176No Hit
AACGATGCCGACCGGCGATGCGGCGGCG420070.10836124911755116No Hit
GGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGG419700.10826580392467022No Hit
CTGCATAATTTGTGGTAGTGGGG419020.1080903911377539No Hit
CAGCGGAGGAAAAGAAACTAAC411610.10617890768032763No Hit
CAGCGGAGGAAAAGAAACTAACCAGGATTCCCTC397360.10250297795450788No Hit
ACCGCAGCTAGGAATAATGGAAT394210.10169040401511614No Hit
GTGAACAGGGAAGAGCCCAGCGCCGAATCCCC389900.10057859649804364No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)