FastQCFastQC Report
Sat 2 Sept 2023
SRR12494988_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12494988_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31120180
Total Bases892.3 Mbp
Sequences flagged as poor quality0
Sequence length18-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACCTCGGGCCGATCGCAC9520283.059198243711958No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC6799582.18494237501197No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTT4674561.5020992809167557No Hit
CGACTGCATAATTTGTGGTAGTGGGG4159021.336438285382668No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT3533521.1354433039911722No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGG3506271.1266869279033733No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC3214981.033085284211081No Hit
AGATAACCTCGGGCCGATCGCAC2658700.8543331047571061No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCG2630890.8453967811240167No Hit
TCGACTGCATAATTTGTGGTAGTGGGG2594830.8338094445469145No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG2486650.7990474348156085No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAC2415160.7760752026498561No Hit
GACTGCATAATTTGTGGTAGTGGGG2062710.6628207163326176No Hit
CACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCT1850070.5944920627065782TruSeq Adapter, Index 12 (100% over 50bp)
ACGATCAAAAGGGACAAGCATCAAGCACGCAGC1728840.5555366325001976No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC1695500.5448233268573639No Hit
CGACTGCATAATTTGTGGTAGTGGGGG1612400.5181203964758558No Hit
AGTCCGCCCGGAGGATTC1397160.44895627210382455No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGC1366230.43901738357554493No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGA1321420.424618366603278No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGG1280820.41157216956971326No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG977860.31422054756752693No Hit
GACTGCATAATTTGTGGTAGTGGGGG973150.31270706017767247No Hit
AGTTGGTCCGAGTGTTGTGGGTTATTGTTA965550.31026491492015795No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCG960670.3086968005969117No Hit
GCTGGTCCGAAGGTAGTGAGTTATCTCAA953940.3065342167044021No Hit
AACCTCGGGCCGATCGCA858330.2758113866950641No Hit
GTCTAGGGGTATGATTCTCGCTT823090.26448754473785174No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGC821960.2641244362982476No Hit
AGCCTGGTGATAGCTGGTTGTCCAAGATAGAAT800540.25724144269088417No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAT787890.2531765561767316No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCG750860.24127752474439415No Hit
ACCCCTCCCACGGCGCGACCGC744460.23922098136964504No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCG728750.23417281005444057No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC701930.22555460797463253No Hit
ACTAAAATTGGAACGATACAGAGAA674400.21670825811418828No Hit
ACCCCTCCCACGGCGCGACCGCTC658160.2114897793007624No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGG626310.2012552626623625No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATC615900.1979101663293721No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGG609660.19590503653899174No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCG590180.1896454326420991No Hit
CGATCAAAAGGGACAAGCATCAAGCACGCAGC589270.18935301788100198No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGC561160.18032029377722109No Hit
GCGCAGCGGAAGCGTGCTGGGC550880.17701697098153032No Hit
ACCACCGGCCCGTCTCGC541470.1739932095508445No Hit
GACCGCCTGGGAATACCGGGTGCTGTAGGCTT535910.1722065874940312No Hit
CTGGTCCGATGGTAGTGGGTTATCAGAA533550.17144823712459248No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGC531200.17069310010417676No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGA521810.16767576537153706No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGA513910.1651372196433311No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTG502790.16156397552970453No Hit
ATCCAGCGATCCGAGTTC496410.15951385885300148No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCA467100.15009553286645516No Hit
ACGATTAAAGTCCTACGTGATCTGAGT461210.14820287029188134No Hit
GCCGGGCGCGGTGGCGCGTGCCTGT459360.1476084007226179No Hit
CTGGTCCGAAGGTAGTGAGTTATCTCAA459000.14749272015778828No Hit
GAGAAATAAGGCCTACTTCAC451670.14513733532389594No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGA435140.13982566938880175No Hit
ACGATTAAAGTCCTACGTGATCTGAGTT433370.1392569066117227No Hit
AAACATCACCTCTAGCATCACC432000.13881667779556545No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGA429020.13785909978669791No Hit
ATGTGTGACTGAAAGGTATTTTCTGAGC425330.13667337399719412No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGG423070.13594715711798583No Hit
ATTCGAACGTCTGCCCTATC422430.13574150278051092No Hit
AAACATCACCTCTAGCATCACCAGTATTAGAGGC422180.13566116905493478No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGA418690.1345397102458919No Hit
CACATATACTAAAATTGGAACGATACAGAGAA408560.1312845876855468No Hit
GACCGGCTCCGGGACGGCTGGG394480.12676019226109875No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCC378690.1216863141537099No Hit
GCTGGTCCGATGGTAGTGGGTTATCAGAA376120.1208604834547872No Hit
ATACTAAAATTGGAACGATACAGAGAA370810.11915419512355005No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAA358110.11507324186428226No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGC349100.11217801439451827No Hit
GGTGACTCTAGATAACCTCGGGCCGATCGCAC348200.1118888129824442No Hit
AGCCTGGTGATAGCTGGTTGTCCAAGATAGAA347080.1115289178918631No Hit
TTTGGTGACTCTAGATAAC344120.11057776658104163No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGA341490.10973265578798067No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGA334270.10741261779334181No Hit
ATGCAGCTCAAAACGCTTAGCCTAGCCACAC331230.10643575969033597No Hit
CACACGTCTGAACTCCAGTCACCTTGTAATGTCGTATGCCGTCTTCTGCT329260.10580272993279602TruSeq Adapter, Index 12 (98% over 50bp)
GCACCACCGGCCCGTCTCGC329040.10573203625428901No Hit
ACATATACTAAAATTGGAACGATACAGAGAA322740.10370762636977035No Hit
GCGACCGGCTCCGGGACGGCTGGG314460.10104697337868869No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)