FastQCFastQC Report
Sat 2 Sept 2023
SRR12507495_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12507495_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42269172
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTA2199720.5204076389289102TruSeq Adapter, Index 1 (100% over 47bp)
CCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTA2110420.4992811309386425TruSeq Adapter, Index 1 (100% over 47bp)
CTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTA1983190.46918118008084003TruSeq Adapter, Index 1 (100% over 47bp)
TTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTA1756180.4154753729266331TruSeq Adapter, Index 1 (100% over 47bp)
GCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTA686390.1623854851001103TruSeq Adapter, Index 1 (100% over 47bp)
TCGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGT684060.16183425594426123TruSeq Adapter, Index 1 (100% over 48bp)
TTGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGT637300.15077182018138421TruSeq Adapter, Index 1 (100% over 48bp)
ACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTA594350.14061075054888703TruSeq Adapter, Index 1 (100% over 47bp)
CTGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGT571730.1352593327354508TruSeq Adapter, Index 1 (98% over 50bp)
CCGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGT524730.12414011800373095TruSeq Adapter, Index 1 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)