FastQCFastQC Report
Fri 1 Sept 2023
SRR12513919_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12513919_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41036616
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length10-47
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGCTGAGGCGTGATGACGAGGCAC649184815.819647506997164No Hit
TAGGCTGAGGCGTGATGACGAGGCAC17258414.205612373106009No Hit
AAGGCTGAGGCGTGATGACGAGGCA14782373.602239034524679No Hit
AAGGCTGAGGCGTGATGACGAGGC10387482.5312710970124828No Hit
TAAGGCTGAGGCGTGATGACGAGGCAC9869342.4050082492182105No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGC6706181.6341942035376404No Hit
AAAGGCTGAGGCGTGATGACGAGGCAC6101791.4869135408241263No Hit
TAGGCTGAGGCGTGATGACGAGGCA3749860.9137839240935461No Hit
TAGGCTGAGGCGTGATGACGAGGC2973260.724538300136639No Hit
AGAGCACTGTTTCGGC2288340.5576336996208459No Hit
TAAGGCTGAGGCGTGATGACGAGGCA2254160.5493045527925596No Hit
CAGGCTGAGGCGTGATGACGAGGCAC2172440.5293906300655981No Hit
GGCTGAGGCGTGATGACGAGGCAC2101950.5122132877623243No Hit
TATTCATCTCCGGGGGTAGAGC2069110.5042106785803196No Hit
AGGCTGAGGCGTGATGACGAGGCAC1924900.46906889203534713No Hit
TAAGGCTGAGGCGTGATGACGAGGC1832990.4466718210877817No Hit
GAGGCTGAGGCGTGATGACGAGGCAC1560280.38021653637327213No Hit
CTGTAGGCACCATCAATATCT1505890.3669625195215902No Hit
ATGTACACGGAGTCGACCCGCAACGCGA1446000.35236823621128993No Hit
AAAGGCTGAGGCGTGATGACGAGGCA1390590.338865660852737No Hit
CTGTAGGCACCAT1349900.32895012590706796No Hit
ACTGTTTCGGC1247670.3040382277135132No Hit
CTGTAGGCACCATCA1191710.29040162570909844No Hit
CTGTAGGCACCATCAAT1121630.2733241941781944No Hit
AAAGGCTGAGGCGTGATGACGAGGC1095670.26699813649351595No Hit
TGATGACGAGGCAC1045290.2547212957325721No Hit
CTGTAGGCACCATCAATAT1011680.24653104924636085No Hit
CAAGGCTGAGGCGTGATGACGAGGCAC986430.24037800777724944No Hit
CATTCATCTCCGGGGGTAGAGCACTGTTTCGGC977740.23826038677263253No Hit
ATCTCCGGGGGTAGAGCACTGTTTCGGC956080.23298217377378289No Hit
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGC952870.23219994553157114No Hit
ACGGTCCTAAGGTAGCG927170.2259372458976637No Hit
AAGGCTGAGGCGTGATGA896690.21850973286881162No Hit
ACGATGTCGACTTGGAGGTTGTGCC838230.20426391883775213No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGG814610.19850808361001307No Hit
TGGCTGAGGCGTGATGACGAGGCAC806890.19662683687173424No Hit
TACTCATCTCCGGGGGTAGAGCACTGTTTCGGC779510.18995474675592158No Hit
TTAGGCTGAGGCGTGATGACGAGGCAC772400.1882221477521441No Hit
AAGGCTGAGGCGTGATGACGAGG770750.1878200678145586No Hit
GATGACGAGGCAC735310.17918387812484343No Hit
GATTCATCTCCGGGGGTAGAGCACTGTTTCGGC629260.1533411039545756No Hit
ACCATGCACCGAAGCTGCGGCAG582420.14192690742336064No Hit
CTGTAGGCACC580470.14145172204257778No Hit
GAAGGCTGAGGCGTGATGACGAGGCAC566120.13795484500963726No Hit
CTGTAGGCACCATCAATATCTCGTATG558290.13604679294218605No Hit
TTGAGGCGAACCGGGGGAACTGA556470.13560328658678875No Hit
AGCACGCCTGGAAAGTGTG551430.13437511514107303No Hit
CTGTAGGCACCATCAATATCTCGT511850.12473007033523427No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACCC511420.12462528586665138No Hit
TACTGTTTCGGC506760.12348971464898567No Hit
TGTGATGACGAGGCAC500040.12185215272136475No Hit
CTGTAGGCACCATC495190.12067028138967403No Hit
CTGTAGGCACCATCAA489170.11920329882951362No Hit
CAGGCTGAGGCGTGATGACGAGGCA482130.11748775776248216No Hit
ACTAACTGGAGGACCGAACCGAC450110.10968497012521697No Hit
CTGTAGGCAC447150.10896366308566965No Hit
GTGATGACGAGGCAC437240.10654874661205009No Hit
AATTCATCTCCGGGGGTAGAGCACTGTTTCGGC435210.10605406644641459No Hit
AGGCTGAGGCGTGATGACGAGGCA432360.10535956473603965No Hit
GGCTGAGGCGTGATGACGAGGCA432210.10532301201444097No Hit
CTGTAGGCACCATCAATATCTC424310.10339790201024372No Hit
AGACCGCCAGCTAAGGTCCCAAAGTCATGG413610.10079047453620445No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)