FastQCFastQC Report
Fri 1 Sept 2023
SRR12513920_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12513920_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28800198
Total Bases728.8 Mbp
Sequences flagged as poor quality0
Sequence length10-47
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGCTGAGGCGTGATGACGAGGCAC337353911.713596552357036No Hit
TAGGCTGAGGCGTGATGACGAGGCAC9287303.2247347743928705No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGC7505652.606110555212155No Hit
AAGGCTGAGGCGTGATGACGAGGCA6750392.3438693025652118No Hit
TAAGGCTGAGGCGTGATGACGAGGCAC6454302.2410609815946407No Hit
AAGGCTGAGGCGTGATGACGAGGC4759551.6526101660828858No Hit
AAAGGCTGAGGCGTGATGACGAGGCAC3932871.3655704728141105No Hit
TATTCATCTCCGGGGGTAGAGC1971350.6844918218965023No Hit
TAGGCTGAGGCGTGATGACGAGGCA1966530.6828182222913883No Hit
TAGGCTGAGGCGTGATGACGAGGC1541460.5352254869914437No Hit
AGGCTGAGGCGTGATGACGAGGCAC1485140.5156700658794082No Hit
TAAGGCTGAGGCGTGATGACGAGGCA1313020.45590658786443067No Hit
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGC1197810.4159033906641892No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGG1197680.415858252085628No Hit
GGCTGAGGCGTGATGACGAGGCAC1141870.39647991308948644No Hit
CAGGCTGAGGCGTGATGACGAGGCAC1088920.3780946228216903No Hit
ATGGCTGAGGCGTGATGACGAGGCAC1075660.37349048780845184No Hit
AGAGCACTGTTTCGGC1046690.3634315291860146No Hit
TAAGGCTGAGGCGTGATGACGAGGC1005350.3490774612035653No Hit
CATTCATCTCCGGGGGTAGAGCACTGTTTCGGC982800.3412476539223793No Hit
TACTCATCTCCGGGGGTAGAGCACTGTTTCGGC956190.33200813411074465No Hit
GAGGCTGAGGCGTGATGACGAGGCAC886740.30789371656403197No Hit
TGATGACGAGGCAC829270.28793899264164785No Hit
ACTGTTTCGGC825700.2866994178303913No Hit
AAAGGCTGAGGCGTGATGACGAGGCA795730.2762932393728682No Hit
GATTCATCTCCGGGGGTAGAGCACTGTTTCGGC708940.24615802988576674No Hit
ACGATGTCGACTTGGAGGTTGTGCC669850.23258520653226064No Hit
GATGACGAGGCAC658030.22848106808154584No Hit
CTGTAGGCACCAT640800.22249847032301653No Hit
AGACCGCCAGCTAAGGTCCCAAAGTCATGG626410.21750197689613107No Hit
CAAGGCTGAGGCGTGATGACGAGGCAC621940.21594990423329727No Hit
TTAGGCTGAGGCGTGATGACGAGGCAC617100.21426936023148177No Hit
AAAGGCTGAGGCGTGATGACGAGGC598260.20772773853846419No Hit
ACCATGCACCGAAGCTGCGGCAG543450.18869661937740845No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACCC513650.17834946829184994No Hit
ACGGTCCTAAGGTAGCG513630.17834252389514824No Hit
AATTCATCTCCGGGGGTAGAGCACTGTTTCGGC481400.1671516286103311No Hit
TGGCTGAGGCGTGATGACGAGGCAC476640.1654988621953224No Hit
TTGGCTGAGGCGTGATGACGAGGCAC453520.1574711396081374No Hit
ACCGCCAGCTAAGGTCCCAAAGTCATGG451530.15678017213631656No Hit
AAGGCTGAGGCGTGATGACGAGG446660.15508921153944843No Hit
AGGCACGCTGATATGTAGGTGAAGCGAC437130.15178020651108023No Hit
TTGAGGCGAACCGGGGGAACTGA435010.15104410046069822No Hit
ATGACGAGGCAC427480.14842953510250173No Hit
CTGTAGGCAC427090.14829411936681824No Hit
CTGTAGGCACC418170.14519691843785243No Hit
AACTGGAGGAAGGTGGGG418140.14518650184279983No Hit
ACTAACTGGAGGACCGAACCGAC411780.14297818369165377No Hit
CTGTAGGCACCATCA389660.13529768093955466No Hit
GAAGGCTGAGGCGTGATGACGAGGCAC381660.1325199222588678No Hit
AAGTCTGAGGCGTGATGACGAGGCAC353130.12261374036386832No Hit
GACGAGGCAC353030.12257901838035974No Hit
ATTCATCTCCGGGGGTAGAGC350760.12179082935471486No Hit
ACTCATCTCCGGGGGTAGAGCACTGTTTCGGC349250.12126652740373522No Hit
CTGTAGGCACCATCAATATCT344710.11969014935244542No Hit
ATCTCCGGGGGTAGAGCACTGTTTCGGC339680.11794363358196357No Hit
TATTCATCTCCGGGGGTAGAGCA339400.11784641202813953No Hit
ACGGGGCTAAACCATGCACCGAAGCTGCGGCAGCGACGC333290.11572489883576495No Hit
TGTGATGACGAGGCAC325350.11296797334518324No Hit
AGGCTGAGGCGTGATGACGAGGCA323760.11241589380739674No Hit
CTGTAGGCACCATCAAT320520.11129090154171856No Hit
GTGATGACGAGGCAC314780.10929785968832575No Hit
TTTTCATCTCCGGGGGTAGAGCACTGTTTCGGC311480.10815203423254244No Hit
TTGAGGCGAACCGGGGGAACTG292090.10141944163022769No Hit
ACCATGCACCGAAGCTGCGGCA289370.10047500367879417No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)