FastQCFastQC Report
Fri 1 Sept 2023
SRR12513921_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12513921_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30543901
Total Bases756.4 Mbp
Sequences flagged as poor quality0
Sequence length10-47
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGCTGAGGCGTGATGACGAGGCAC547847717.93640242613411No Hit
TAGGCTGAGGCGTGATGACGAGGCAC18193135.956387168750973No Hit
TAAGGCTGAGGCGTGATGACGAGGCAC11830903.873408311531654No Hit
AAGGCTGAGGCGTGATGACGAGGCA10375233.396825441517768No Hit
AAGGCTGAGGCGTGATGACGAGGC8999822.946519503189851No Hit
AAAGGCTGAGGCGTGATGACGAGGCAC7682592.515261557454629No Hit
TAGGCTGAGGCGTGATGACGAGGCA3486721.1415437733379243No Hit
TAGGCTGAGGCGTGATGACGAGGC3171101.038210541606981No Hit
CAGGCTGAGGCGTGATGACGAGGCAC2385210.7809120387078259No Hit
TAAGGCTGAGGCGTGATGACGAGGCA2308480.7557908205634899No Hit
TAAGGCTGAGGCGTGATGACGAGGC2053580.6723371713390506No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGC1898570.6215872687643926No Hit
GGCTGAGGCGTGATGACGAGGCAC1893590.6199568286971596No Hit
GAGGCTGAGGCGTGATGACGAGGCAC1773910.5807738834669481No Hit
ATGGCTGAGGCGTGATGACGAGGCAC1762820.5771430440401178No Hit
AAAGGCTGAGGCGTGATGACGAGGCA1419170.4646328574729207No Hit
TATTCATCTCCGGGGGTAGAGC1383620.45299387265562446No Hit
CAAGGCTGAGGCGTGATGACGAGGCAC1268750.4153857098999896No Hit
AAAGGCTGAGGCGTGATGACGAGGC1223320.4005120367565361No Hit
AGGCTGAGGCGTGATGACGAGGCAC1203170.39391497503871553No Hit
ACGGTCCTAAGGTAGCG1192110.3902939575400012No Hit
TTAGGCTGAGGCGTGATGACGAGGCAC914460.2993920128277No Hit
TGGCTGAGGCGTGATGACGAGGCAC730110.23903626455572913No Hit
TTGGCTGAGGCGTGATGACGAGGCAC700850.22945661066672524No Hit
TGATGACGAGGCAC638050.20889604114418783No Hit
AAGTCTGAGGCGTGATGACGAGGCAC576820.18884948585971387No Hit
AGAGCACTGTTTCGGC570150.1866657438419539No Hit
AAGGCTGAGGCGTGATGAC568350.1860764281550022No Hit
GAAGGCTGAGGCGTGATGACGAGGCAC559580.18320515116913194No Hit
GATGACGAGGCAC516070.16896008142509367No Hit
CAGGCTGAGGCGTGATGACGAGGCA464540.15208928289808168No Hit
TAACCGTGAGGCTTAACC448690.146900030876868No Hit
AAAGTCTGAGGCGTGATGACGAGGCAC425450.13929131056311372No Hit
CAGGCTGAGGCGTGATGACGAGGC423950.13880021415732063No Hit
AAGGGTGAGGCGTGATGACGAGGCAC422040.1381748847339441No Hit
AAGACTGAGGCGTGATGACGAGGCAC408880.1338663322671194No Hit
ACTAACTGGAGGACCGAACCGAC399460.13078224683873876No Hit
GTGATGACGAGGCAC381990.12506261069926858No Hit
AAGGCTGAGGCGTGATGACGAGG380100.12444382922796927No Hit
ACCATGCACCGAAGCTGCGGCAG348990.11425848977182057No Hit
GGCTGAGGCGTGATGACGAGGCA342450.1121173094425627No Hit
GAGGCTGAGGCGTGATGACGAGGCA333640.10923293655253795No Hit
ATGGCTGAGGCGTGATGACGAGGCA325580.10659411186540974No Hit
TTAAGGCTGAGGCGTGATGACGAGGCAC315190.10319245076128292No Hit
AGGCTGAGGCGTGATGACGAGGC311720.10205638107588157No Hit
CATTCATCTCCGGGGGTAGAGCACTGTTTCGGC310060.10151290105347055No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)