FastQCFastQC Report
Fri 1 Sept 2023
SRR12513922_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12513922_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29380918
Total Bases736.2 Mbp
Sequences flagged as poor quality0
Sequence length10-47
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGCTGAGGCGTGATGACGAGGCAC510149517.363293413772844No Hit
TAGGCTGAGGCGTGATGACGAGGCAC15997305.444792432966185No Hit
TAAGGCTGAGGCGTGATGACGAGGCAC10291243.5026951846773473No Hit
AAGGCTGAGGCGTGATGACGAGGCA10007803.406224407283666No Hit
AAGGCTGAGGCGTGATGACGAGGC8585222.9220393998580985No Hit
AAAGGCTGAGGCGTGATGACGAGGCAC6186652.105669400799526No Hit
TAGGCTGAGGCGTGATGACGAGGCA3181971.0830056433226491No Hit
TAGGCTGAGGCGTGATGACGAGGC2794870.9512534632171806No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGC2424350.8251444015466092No Hit
CAGGCTGAGGCGTGATGACGAGGCAC2288150.7787877832816523No Hit
TAAGGCTGAGGCGTGATGACGAGGCA2255080.767532178538465No Hit
TAAGGCTGAGGCGTGATGACGAGGC1958860.6667116391666182No Hit
ATGGCTGAGGCGTGATGACGAGGCAC1707340.5811050560094821No Hit
TATTCATCTCCGGGGGTAGAGC1645180.5599484672330524No Hit
GGCTGAGGCGTGATGACGAGGCAC1588200.5405549275213253No Hit
GAGGCTGAGGCGTGATGACGAGGCAC1553030.5285845731573124No Hit
AAAGGCTGAGGCGTGATGACGAGGCA1235420.4204837983619164No Hit
ACGGTCCTAAGGTAGCG1208470.41131117822799135No Hit
CAAGGCTGAGGCGTGATGACGAGGCAC1200600.40863256893470784No Hit
AGGCTGAGGCGTGATGACGAGGCAC1058240.3601793517819967No Hit
AAAGGCTGAGGCGTGATGACGAGGC1039750.3538861515491109No Hit
TTAGGCTGAGGCGTGATGACGAGGCAC854750.29092011352402264No Hit
TTGGCTGAGGCGTGATGACGAGGCAC655120.22297465314051793No Hit
TGGCTGAGGCGTGATGACGAGGCAC641320.21827772706080867No Hit
AAGGCTGAGGCGTGATGAC621450.21151483421995185No Hit
TGATGACGAGGCAC587050.19980655471690842No Hit
AGAGCACTGTTTCGGC581110.19778483436085964No Hit
AAGTCTGAGGCGTGATGACGAGGCAC526130.17907200857372804No Hit
ACTAACTGGAGGACCGAACCGAC515470.17544380335563375No Hit
GAAGGCTGAGGCGTGATGACGAGGCAC509020.17324850094881308No Hit
GATGACGAGGCAC476840.16229581390207073No Hit
CAGGCTGAGGCGTGATGACGAGGCA471990.16064508263492652No Hit
AAGGCTGAGGCGTGATGACGAGG444680.1513499339945743No Hit
TACTCATCTCCGGGGGTAGAGCACTGTTTCGGC430030.14636370449691188No Hit
CATTCATCTCCGGGGGTAGAGCACTGTTTCGGC428300.14577488695213675No Hit
CAGGCTGAGGCGTGATGACGAGGC424110.14434879128010908No Hit
AAGGGTGAGGCGTGATGACGAGGCAC375100.12766789655789518No Hit
AAGACTGAGGCGTGATGACGAGGCAC374400.12742964668428672No Hit
AAAGTCTGAGGCGTGATGACGAGGCAC341640.11627955259941164No Hit
GTGATGACGAGGCAC340410.11586091353578537No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGG331230.11273643662189181No Hit
ATGGCTGAGGCGTGATGACGAGGCA326490.11112314462060034No Hit
AAGGCTGAGGCGTGAT324730.11052411636695626No Hit
GGCTGAGGCGTGATGACGAGGCA310110.10554809757816282No Hit
CAAGGCTGAGGCGTGATGACGAGGCA308960.10515668707152036No Hit
AGGCACGCTGATATGTAGGTGAAGCGAC302830.10307029889263503No Hit
GAGGCTGAGGCGTGATGACGAGGCA299730.10201519230951192No Hit
TTAAGGCTGAGGCGTGATGACGAGGCAC299170.10182459241062515No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)