FastQCFastQC Report
Fri 1 Sept 2023
SRR12513923_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12513923_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32746654
Total Bases721.8 Mbp
Sequences flagged as poor quality0
Sequence length10-47
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCAGGTAACATCAAATCGTACCCC2690180.8215129399174647No Hit
TATGCGTTGTTGGGT2668480.8148863086897367No Hit
ATGCGTTGTTGGGT2232640.6817917946670216No Hit
AAATGAACCGTGAGGCTTAACC2221250.6783135767092419No Hit
TTGAGGCGTGGCTTCCGGAGCTAACGCGTT1808330.5522182510616199No Hit
TAGGCGTGTGACTGCGTACCTTTTGT1776030.5423546478977669No Hit
ATTTAGGTAGCGCCTCGTGA1629220.49752258658243376No Hit
GGTTAAGCGACTAAGCGTAC1544760.4717306384951574No Hit
AGTGGGAGCACGC1407190.4297202395090503No Hit
ACCTGCGGTTGGATCACCTCC1243920.3798617104513945No Hit
ACAAGCAGTGGGAGCACGC1236150.3774889489472726No Hit
AAGCAGTGGGAGCACGC1191560.36387229058578013No Hit
GGTTAAGCGACTAAGCGT1160360.35434459960397785No Hit
AGGTAGCGCCTCGTGA1155570.3528818547385024No Hit
AGGGAACTGCCAGGCAT1152830.35204512803048515No Hit
ATCAGGTAACATCAAATCGT1140900.3484020077287896No Hit
AAATTGAAGAGTTTGATC1098930.33558543110999983No Hit
TAGGCGTGTGACTGCGTACCTTTTGTAT1001450.3058175042860867No Hit
AAGGCTGAGGCGTGATGACGAGGCAC982420.3000062235366092No Hit
TAGACTCCTACGGGAGGCAGC847770.25888751870649135No Hit
TTGAGGCGTGGCTTCCGGAGCT832550.2542397156057532No Hit
ACGTACAAGCAGTGGGAGCACGC808700.24695652874947163No Hit
ATGGGGGGACCATCCTCCAAGGCT773460.2361951239354103No Hit
TGATGACGAGGCAC731160.22327777366200527No Hit
AGCGCCTCGTGA687980.21009169364295965No Hit
AAATTGAAGAGTTTGATCATGGCTC674670.20602715624014595No Hit
AGGCGTGTGACTGCGTACCTTTTGT626630.19135695512585804No Hit
AAATTGATGGGGTTAGCGC621620.18982702782397248No Hit
AAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGC612660.18709087041381386No Hit
TAGTCGATGGGAAAC595240.18177124294897426No Hit
AGGGCGGGACACGTGGTATCCTGTCTGAAT577220.17626839065756153No Hit
ATCAGGTAACATC572050.17468960340192313No Hit
ATGTGTTGTTGGGT553890.16914399865097668No Hit
ATGACGAGGCAC547140.16708271935202906No Hit
AGGCGTGTGACTGCGT545310.16652388363098106No Hit
AAAATTGAAGAGTTTGATC535160.16342433031478576No Hit
TAGGCTGGTTTTCCAGGCAAATCCGGAAA528420.1613661047629477No Hit
ATAACGGTCCTAAGGT512560.15652286184719819No Hit
TAATGAACCGTGAGGCTTAACC510180.1557960700351248No Hit
AAATGGGCGCAAGCCTGATGC496040.15147807162221827No Hit
GATGACGAGGCAC484910.14807925108928688No Hit
ACGGTCCTAAGGTAGCG484020.14780746759653673No Hit
AGGGGAGCGTTCTGT479860.1465371087989631No Hit
ATCAGGTAACATCAAATCGTACCCCAAACCGAC476000.145358362414676No Hit
TAGGCGTGTGACTGCGT475640.14524842751873215No Hit
AGCTGGTCTGAGAGGATGACCAGCCAC449640.1373086850338969No Hit
AAAATTGAAGAGTTTGATCATGGCTC446120.1362337660513346No Hit
ATCAGGTAACATCAAATC446000.13619712108601995No Hit
ATGACTGGGGTGAAGTCGTAAC445290.13598030504124176No Hit
TAGGGAACTGCCAGGCAT439490.1342091317177016No Hit
TAGGGTGAGTCGACCCCT437410.13357395231891478No Hit
AGTGGGAGCATGC428850.13095994479313824No Hit
ACTAATGAACCGTGAGGCTTAACC428280.13078588120789378No Hit
AAGCGTGTAGGCTG424800.1297231772137697No Hit
GGTTAAGCGACTAAGCGTACAC424060.12949719992766284No Hit
AGTGGGGAATATTGCAC420300.12834899101447128No Hit
ATAACGGTCCT411320.12560672611009355No Hit
AAAAAGGTACTCCGGGGATAAC406170.12403404634867428No Hit
ACGTGCTACAATGGCGCATAC403690.12327671706550537No Hit
ATGCGTTGTTGGGTAGGGGAGCGTTCTGT402230.12283086998751079No Hit
GGTTAAGCGACT399000.12184450967112548No Hit
CTGTAGGCACCAT397230.12130399643273478No Hit
ACGCTGATATGTAGGTGAAGCGAC385620.11775859603854488No Hit
AAATTGATGGGGTTAGCG382040.11666535457332526No Hit
AGGCGTGTGACTGCGTACCTTTTGTAT375980.11481478382493675No Hit
AAGGGGGTCATCCCGACTTACC369250.11275961202020822No Hit
AGTAATCGTGGATC364060.11117471727035072No Hit
ACTGGAACTGAGACACGGTCC362740.11077162265188986No Hit
TCCTGCGGTTGGATCACCTCC352160.10754075820998384No Hit
TGAGGCGTGGCTTCCGGAGCTAACGCGTT348880.10653912915805078No Hit
ACTGTTTCGGC347180.10601999214942692No Hit
ACCAACCCGATGC345270.1054367264515025No Hit
AAATGAACCGTGAGGCTTAAC344290.1051374592347664No Hit
ACTAACTGGAGGACCGAACCGAC343870.1050092018561652No Hit
AGGCTGGTTTTCCAGGCAAATCCGGAAA337520.10307007244159969No Hit
AAGTCGAACGGTAAC336780.10284409515549284No Hit
TATTCATCTCCGGGGGTAGAGC335910.10257841915696181No Hit
TAGTGTCTGGTGGGT333760.10192186352840814No Hit
ATCAAATCGTACCCC331280.10116453424523922No Hit
ACGAATGGCGTAATGATGGCC328740.10038888247941302No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)