FastQCFastQC Report
Fri 1 Sept 2023
SRR12513924_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12513924_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32912858
Total Bases811.4 Mbp
Sequences flagged as poor quality0
Sequence length10-47
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGCTGAGGCGTGATGACGAGGCAC439904013.36571865013971No Hit
TAGGCTGAGGCGTGATGACGAGGCAC14750524.481689192716111No Hit
TAAGGCTGAGGCGTGATGACGAGGCAC9226312.8032539744801257No Hit
AAGGCTGAGGCGTGATGACGAGGCA6128271.8619683529154472No Hit
AAAGGCTGAGGCGTGATGACGAGGCAC5599291.7012469716242813No Hit
AAGGCTGAGGCGTGATGACGAGGC4326541.3145439997948523No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGC4230821.285461141053141No Hit
TATTCATCTCCGGGGGTAGAGC2699720.8202630108877205No Hit
ACGGTCCTAAGGTAGCG2104460.6394036032969243No Hit
TAGGCTGAGGCGTGATGACGAGGCA2044450.621170607547968No Hit
CAGGCTGAGGCGTGATGACGAGGCAC1794710.5452914481021368No Hit
TAGGCTGAGGCGTGATGACGAGGC1768640.5373705316019655No Hit
GAGGCTGAGGCGTGATGACGAGGCAC1580600.4802378450391638No Hit
GGCTGAGGCGTGATGACGAGGCAC1515450.46044314960432786No Hit
ATGGCTGAGGCGTGATGACGAGGCAC1378370.418793773545889No Hit
TAAGGCTGAGGCGTGATGACGAGGCA1259310.38261946136674No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGG1102040.3348357046355561No Hit
TAACCGTGAGGCTTAACC1059270.32184078331939453No Hit
TAAGGCTGAGGCGTGATGACGAGGC966800.29374538060474725No Hit
TTAGGCTGAGGCGTGATGACGAGGCAC941990.28620729321045285No Hit
AGGCTGAGGCGTGATGACGAGGCAC909420.2763114646561535No Hit
CAAGGCTGAGGCGTGATGACGAGGCAC898850.27309995382351787No Hit
TGGCTGAGGCGTGATGACGAGGCAC868010.2637297557082402No Hit
TGATGACGAGGCAC811600.24659055740464714No Hit
AAAGGCTGAGGCGTGATGACGAGGCA774820.23541559350452032No Hit
TACGGTCCTAAGGTAGCG748750.2274946770043489No Hit
TTGGCTGAGGCGTGATGACGAGGCAC702150.21333607673937036No Hit
AGAGCACTGTTTCGGC697760.21200225152127475No Hit
ACCATGCACCGAAGCTGCGGCAG645110.19600546388283874No Hit
AGGTAGCGCCTCGTGA638810.19409131835345322No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACCC616270.18724293101498507No Hit
TAAGTCTGAGGCGTGATGACGAGGCAC585400.1778636179209961No Hit
AAAGGCTGAGGCGTGATGACGAGGC570980.17348235148706928No Hit
CATTCATCTCCGGGGGTAGAGCACTGTTTCGGC570830.17343677659351248No Hit
TACTCATCTCCGGGGGTAGAGCACTGTTTCGGC569760.17311167568614066No Hit
AACTGGAGGAAGGTGGGG568780.17281391971490292No Hit
ACTAACTGGAGGACCGAACCGAC563910.17133425483742554No Hit
TTGAGGCGAACCGGGGGAACTGA555490.16877598414577064No Hit
GAAGGCTGAGGCGTGATGACGAGGCAC543920.16526064068942295No Hit
ACGATGTCGACTTGGAGGTTGTGCC542500.16482919836375193No Hit
ACTAACTGGAGGACCG495180.15045183860970082No Hit
AGACCGCCAGCTAAGGTCCCAAAGTCATGG487880.14823386045660333No Hit
GATGACGAGGCAC486220.1477294983012414No Hit
ATGTACACGGAGTCGACCCGCAACGCGA478230.14530187563778266No Hit
ATGACGAGGCAC460810.14000911133271987No Hit
AAGTCTGAGGCGTGATGACGAGGCAC454110.13797343275384957No Hit
TATTCATCTCCGGGGGTAGAGCA433730.13178132388259933No Hit
ACTGTTTCGGC427550.12990363826805926No Hit
GATTCATCTCCGGGGGTAGAGCACTGTTTCGGC420780.12784669140552912No Hit
TTGAGGCGAACCGGGGGAACTG407050.12367506948196356No Hit
ACCGCCAGCTAAGGTCCCAAAGTCATGG407010.12366291617701508No Hit
AGGTAACATCAAATCGTACCCC384690.11688137201576357No Hit
ACGGTCCTAAGGTAGCGA380660.11565692654220427No Hit
AGGCACGCTGATATGTAGGTGAAGCGAC377910.1148213868269963No Hit
GACGAGGCAC372720.11324449550993111No Hit
TGTGATGACGAGGCAC370610.11260340867389881No Hit
TTGAGGCGAACCGGGGG366550.11136984822162817No Hit
CATTCATCTCCGGGGGTAGAGC365190.11095663585337985No Hit
ACGGGGCTAAACCATGCACCGAAGCTGCGGCAGCGACGC360730.1096015423516244No Hit
ACCGGAGTGGACGCATCACTGGTGTTCGGGT357010.10847128499141583No Hit
AAGGCTGAGGCGTGATGACGAGG353490.10740179415594962No Hit
TTAGGTAGCGCCTCGTGA352870.10721341792924818No Hit
TACTCATCTCCGGGGGTAGAGC346860.10538738386073918No Hit
ACCATGCACCGAAGCTGCGGC345670.1050258230385219No Hit
ACTGGAGGAAGGTGGGG338420.10282303651661No Hit
AAGGGTGAGGCGTGATGACGAGGCAC330250.1003407239808831No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)