FastQCFastQC Report
Fri 1 Sept 2023
SRR12513925_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12513925_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28787651
Total Bases713.9 Mbp
Sequences flagged as poor quality0
Sequence length10-47
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGCTGAGGCGTGATGACGAGGCAC27987659.722102716890655No Hit
TAGGCTGAGGCGTGATGACGAGGCAC9431033.276067922318497No Hit
AAGGCTGAGGCGTGATGACGAGGCA7924982.7529095722328996No Hit
AAGGCTGAGGCGTGATGACGAGGC7074202.457373128498744No Hit
TAAGGCTGAGGCGTGATGACGAGGCAC6951332.4146916328810573No Hit
TACACTGAGGCGTGACGACGAGGCAC5611091.9491308964388931No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGC4512871.5676409304809205No Hit
AAAGGCTGAGGCGTGATGACGAGGCAC3650281.2680020332329303No Hit
TAGGCTGAGGCGTGATGACGAGGCA2873290.998098108108925No Hit
TAGGCTGAGGCGTGATGACGAGGC2628900.9132040679526092No Hit
TAAGGCTGAGGCGTGATGACGAGGCA2005150.6965313008692512No Hit
TAAGGCTGAGGCGTGATGACGAGGC1995010.6930089572087698No Hit
AACACTGAGGCGTGACGACGAGGCAC1819010.6318716313463714No Hit
ACACTGAGGCGTGACGACGAGGCAC1458030.5064775865179135No Hit
TATTCATCTCCGGGGGTAGAGC1436120.49886668419038427No Hit
TACACTGAGGCGTGACGACGAGGC1421150.49366653778038366No Hit
CAGGCTGAGGCGTGATGACGAGGCAC1406910.4887199723242442No Hit
GGCTGAGGCGTGATGACGAGGCAC1140390.39613860818307134No Hit
GAGGCTGAGGCGTGATGACGAGGCAC1113160.38667969123288315No Hit
TACACTGAGGCGTGACGACGAGGCA1098250.38150038709306294No Hit
ATGGCTGAGGCGTGATGACGAGGCAC1024420.35585397363612614No Hit
AAAGGCTGAGGCGTGATGACGAGGCA1019780.35424217140884473No Hit
AAAGGCTGAGGCGTGATGACGAGGC966250.33564739269626415No Hit
CAAGGCTGAGGCGTGATGACGAGGCAC893810.31048382516517237No Hit
CATTCATCTCCGGGGGTAGAGCACTGTTTCGGC816060.28347571672311855No Hit
TACACTGAGGCGTGATGACGAGGCAC812310.28217307483684584No Hit
ATGACGAGGCAC800340.2780150419358634No Hit
TGATGACGAGGCAC781260.2713872000185079No Hit
TACTCATCTCCGGGGGTAGAGCACTGTTTCGGC742270.25784319811296863No Hit
TTAGGCTGAGGCGTGATGACGAGGCAC726420.25233736507365606No Hit
CACACTGAGGCGTGACGACGAGGCAC692560.2405753772685378No Hit
AGAGCACTGTTTCGGC649350.2255654690269797No Hit
AGGCTGAGGCGTGATGACGAGGCAC611130.21228894292208833No Hit
TGGCTGAGGCGTGATGACGAGGCAC608210.21127461910664405No Hit
ACTGTTTCGGC553610.1923081532425136No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGG552070.1917732016412176No Hit
GAAGGCTGAGGCGTGATGACGAGGCAC544210.18904286424759006No Hit
GATTCATCTCCGGGGGTAGAGCACTGTTTCGGC519080.18031342675371465No Hit
GATGACGAGGCAC513880.1785070966714165No Hit
TTGGCTGAGGCGTGATGACGAGGCAC506470.17593307630414168No Hit
AAGGCTGAGGCGTGATGACGAGG497230.17272336669636573No Hit
AACACTGAGGCGTGACGACGAGGC457700.1589917843592032No Hit
CAGGCTGAGGCGTGATGACGAGGCA440410.15298573683556188No Hit
TAAGTCTGAGGCGTGATGACGAGGCAC425210.14770569505653655No Hit
CAGGCTGAGGCGTGATGACGAGGC421280.14634052635972278No Hit
GACGAGGCAC416800.144784303519589No Hit
GCGTGATGACGAGGCAC381390.13248389040147804No Hit
ACACTGAGGCGTGACGACGAGGC369100.12821469872620034No Hit
TGTGATGACGAGGCAC363890.12640489493220547No Hit
GTGATGACGAGGCAC361280.1254982561793597No Hit
GGCTGAGGCGTGATGACGAGGC358600.1245673014446368No Hit
AACACTGAGGCGTGACGACGAGGCA358250.12444572153525134No Hit
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGC355180.1233792920443561No Hit
ACGATGTCGACTTGGAGGTTGTGCC354040.1229832889109292No Hit
GACACTGAGGCGTGACGACGAGGCAC348260.12097548355022088No Hit
GAGGCTGAGGCGTGATGACGAGGCA344980.11983610611369439No Hit
GGCTGAGGCGTGATGACGAGGCA332470.11549049278108867No Hit
AAGACTGAGGCGTGATGACGAGGCAC321420.11165204135620513No Hit
AATTCATCTCCGGGGGTAGAGCACTGTTTCGGC320380.1112907753397455No Hit
CAAGGCTGAGGCGTGATGACGAGGC308760.10725432234814851No Hit
AAGTCTGAGGCGTGATGACGAGGCAC304760.10586483766945765No Hit
ATGTACACGGAGTCGACCCGCAACGCGA297650.10339502865308461No Hit
ACACTGAGGCGTGACGACGAGGCA295420.10262039094471447No Hit
TTGACGAGGCAC291620.10130038049995813No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)