FastQCFastQC Report
Fri 1 Sept 2023
SRR12513927_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12513927_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28123244
Total Bases697.1 Mbp
Sequences flagged as poor quality0
Sequence length10-47
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGCTGAGGCGTGATGACGAGGCAC519008518.454787790483916No Hit
TAGGCTGAGGCGTGATGACGAGGCAC13989094.974209234183653No Hit
TAAGGCTGAGGCGTGATGACGAGGCAC10139263.6052953208385206No Hit
AAGGCTGAGGCGTGATGACGAGGCA10082783.585212289165503No Hit
AAGGCTGAGGCGTGATGACGAGGC8498633.0219237866015742No Hit
AAAGGCTGAGGCGTGATGACGAGGCAC6388422.2715800495846072No Hit
TAGGCTGAGGCGTGATGACGAGGCA2748970.9774725845994153No Hit
TAGGCTGAGGCGTGATGACGAGGC2238840.7960817038034446No Hit
TAAGGCTGAGGCGTGATGACGAGGCA2121780.7544577716567833No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGC2104420.7482849418082779No Hit
ATGGCTGAGGCGTGATGACGAGGCAC1916990.6816390029542823No Hit
TAAGGCTGAGGCGTGATGACGAGGC1768220.6287397001569236No Hit
CAGGCTGAGGCGTGATGACGAGGCAC1564770.5563974056477979No Hit
AGGCTGAGGCGTGATGACGAGGCAC1555940.5532576540601077No Hit
ACGGTCCTAAGGTAGCG1378710.49023860831986527No Hit
AAAGGCTGAGGCGTGATGACGAGGCA1269830.45152330221933146No Hit
TATTCATCTCCGGGGGTAGAGC1212270.4310562465695636No Hit
GGCTGAGGCGTGATGACGAGGCAC1139910.40532664012729114No Hit
GAGGCTGAGGCGTGATGACGAGGCAC1139770.4052768592414161No Hit
AAAGGCTGAGGCGTGATGACGAGGC1039080.36947373496457236No Hit
CAAGGCTGAGGCGTGATGACGAGGCAC985120.3502867592373056No Hit
AAGGCTGAGGCGTGATGAC893570.31773361565258973No Hit
AGAGCACTGTTTCGGC737720.26231682234097886No Hit
TAACCGTGAGGCTTAACC719810.25594842472653584No Hit
TTAGGCTGAGGCGTGATGACGAGGCAC704240.2504120790617185No Hit
TAAGTCTGAGGCGTGATGACGAGGCAC656630.2334830220866412No Hit
TGATGACGAGGCAC629570.2238610880025078No Hit
GATGACGAGGCAC605880.21543745095693798No Hit
TTGGCTGAGGCGTGATGACGAGGCAC596400.212066573827685No Hit
AAGTCTGAGGCGTGATGACGAGGCAC452170.1607815940437028No Hit
GAAGGCTGAGGCGTGATGACGAGGCAC435550.15487189173482263No Hit
AAGGGTGAGGCGTGATGACGAGGCAC427690.15207705057069518No Hit
AAGGCTGAGGCGTGAT424550.1509605364160692No Hit
GTGATGACGAGGCAC420050.1493604365129428No Hit
TGGCTGAGGCGTGATGACGAGGCAC418300.1487381754395048No Hit
ACTAACTGGAGGACCGAACCGAC413560.1470527368748783No Hit
ATTCATCTCCGGGGGT406910.14468814479581374No Hit
AAGGCTGAGGCGTGATGACGAGG390300.13878199826449608No Hit
ATGGCTGAGGCGTGATGACGAGGCA377220.13413104121274202No Hit
AGGCTGAGGCGTGATGACGAGGC353990.12587096993504732No Hit
AAAGTCTGAGGCGTGATGACGAGGCAC351810.12509581042642165No Hit
AGGCTGAGGCGTGATGACGAGGCA339580.12074709446748035No Hit
AAGACTGAGGCGTGATGACGAGGCAC332920.11837894661085328No Hit
AGGTAGCGCCTCGTGA313020.1113029492614721No Hit
CAGGCTGAGGCGTGATGACGAGGCA312990.1112922819287846No Hit
TACTCATCTCCGGGGGTAGAGCACTGTTTCGGC310810.11051712242015892No Hit
ATGGCTGAGGCGTGATGACGAGGC296180.10531501984621688No Hit
CATTCATCTCCGGGGGTAGAGCACTGTTTCGGC292580.10403493992371578No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)