FastQCFastQC Report
Fri 29 Sept 2023
SRR12538975_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12538975_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21436142
Total Bases845.4 Mbp
Sequences flagged as poor quality0
Sequence length35-151
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14822766.914845031349391No Hit
TTTTACGATCTGTTGAGGCTTATCCCTAGTGGTAAAGTGTTT7823733.649784555448457No Hit
TTTACGATCTGTTGAGGCTTATCCCTAGTGGTANN6700453.1257723521331404No Hit
CTTAAGGAATTATAACAAAGAAGTAACACGTANNN5913942.758863978415519No Hit
TTTACGATCTGTTGAGGCTTATCCCTAGTGGTAAAGTGTTT3621451.689413141599827No Hit
TTTTACGATCTGTTGAGGCTTATCCCTAGTGGTAN3479361.6231278930695643No Hit
AATCCCCACTTTGCATACAATACCGGTAAGCAANN3360831.5678334282353608No Hit
CTTAAGGAATTATAACAAAGAAGTAACACGTNNNN2756541.2859310224759661No Hit
AGATTGTGCTGACTACGTCCCTGCCCTTTGTTTAAGGAATTATAACAAAG2569061.1984712547621674No Hit
GTTTTACGATCTGTTGAGGCTTATCCCTAGTGGTAAAGTGTTT2027800.9459724609027128No Hit
TTTTACGATCTGTTGAGGCTTATCCCTAGTNNNNN2023400.9439198527421585No Hit
ACTAAAGATTGCCGTGTAGTAAGTATGTTNNNNNN1933180.9018320554137028No Hit
TTTACGATCTGTTGAGGCTTATCCCTAGTNNNNNN1534970.7160663518649951No Hit
ATCCCCACTTTGCATACAATACCGGTAAGCAANNN1507200.7031115953607696No Hit
AGATTGTGCTGACTACGTCCCTGCCCTTTGTCTTAAGGAATTATAACAAA1411310.6583787325163268No Hit
CTTTATTGTAGATTTGTGTGGTGTTNNNNNNNNNN1238970.5779818028822538No Hit
ATCTTTGGATAAGTATTTGTTAGGCCTTNNNNNNN1130320.5272963763722036No Hit
TTGTTTTACGATCTGTTGAGGCTTATCCCTAGTGGTAAAGTGTTT1108570.5171499610331001No Hit
TTTTACGATCTGTTGAGGCTTATCCCTAGTGGTAAAGTGTTTT1085330.5063084579305362No Hit
TTTACGATCTGTTGAGGCTTATCCCTAGTGGTNNN1012130.472160522168588No Hit
AAAAGATCTTGAAGCTGTATGCCAACCNNNNNNNN984410.45922909075709606No Hit
AGAAAAAAGTAGGTAAGGGAAGTCGGCNNNNNNNN948490.44247234413729863No Hit
GTTTTACGATCTGTTGAGGCTTATCCCTAGTNNNN942210.4395427124899621No Hit
TTTTACGATCTGTTGAGGCTTATCCCTANNNNNNN859760.40107963457230317No Hit
AATTGTGCTCCAGGAACTTTGATGGCTTCTCNNNN816170.3807448187271758No Hit
TTACGATCTGTTGAGGCTTATCCCTAGTGGTANNN795120.37092495468634235No Hit
AAGGACTGGAGAGCAGGTAGATGTGCATTATCCNN724690.338069229061834No Hit
AGGACTGGAGAGCAGGTAGATGTGCATTATCCNNN718740.335293543026539No Hit
TTAAGGAATTATAACAAAGAAGTAACACGTANNNN649800.3031329051654911No Hit
TTTTACGATCTGTTGAGGCTTATCCCTAGTGGTNN641840.29941955040230656No Hit
TGTTTTACGATCTGTTGAGGCTTATCCCTAGTGGTAAAGTGTTT627240.29260862332410376No Hit
TTTATTGTAGATTTGTGTGGTGTTNNNNNNNNNNN572470.26705831674374986No Hit
AAGAAGATTAAGAACACAATGTGAAAGAGCNNNNN569030.2654535503636802No Hit
TTTACGATCTGTTGAGGCTTATCCCTANNNNNNNN507070.23654909544823877No Hit
TTTACGATCTGTTGAGGCTTATCCCTAGTGGTAAAGTGTTTT490430.22878650458650626No Hit
ATGCTTCCGATCCAGAATACCGTAAACACTNNNNN482080.2248912140999999No Hit
TTAAGGAATTATAACAAAGAAGTAACACGTNNNNN481650.2246906183024912No Hit
AATCCCCACTTTGCATACAATACCGGTAAGCANNN480140.22398620050193735No Hit
ACTCTAGTCTGGCTTTGTGAAACGACTNNNNNNNN471860.22012356514525797No Hit
AACGGTGCCTTCTCAGAACTCTTGTCTTCACANNN465180.21700733275605283No Hit
TTTTACGATCTGTTGAGGCTTATCCCTNNNNNNNN451570.21065824251397477No Hit
AACGATTGGTGCCGACTTTTTAACTAAAGAANNNN440980.20571798787300438No Hit
CCAAATCGTCTAGTGGTTAGGACTCCACGCTNNNN415180.19368224002248166No Hit
AAAAGATCTTGAAGCTGTATGCCAACCANNNNNNN413470.19288452185099353No Hit
ATTAGGCTGTGCGACTGGATCTGTTATGTCTCNNN407180.18995022518511026No Hit
AACGATTGGTGCCGACTTTTTAACTAAAGAAANNN404420.18866268006621714No Hit
GTTTTACGATCTGTTGAGGCTTATCCCTANNNNNN393600.18361512999867236No Hit
AATCCCCACTTTGCATACAATACCGGTAAGCAAGGTGGGGAGTTTGACTG393360.1835031695535512No Hit
ACGATCTGTTGAGGCTTATCCCTAGTGGTNNNNNN386790.18043825236835995No Hit
ATTAGGCTGTGCGACTGGATCTGTTATGTCNNNNN368960.17212052429956845No Hit
AATCACGTTTAGGGGCGAAAGACTNNNNNNNNNNN368380.17184995322385904No Hit
ATCAGACTACATTTGGAATTCTACTTTTATGTNNN360150.16801064295991322No Hit
GAAAGATATGATGAATTGTTTGGACAAGANNNNNN350980.16373282095257627No Hit
GACGTGGCATCTTTTCGGGCTAGTGAAGATTTNNN346320.16155892230980742No Hit
TGTTTTACGATCTGTTGAGGCTTATCCCTAGTNNN332900.1552984674201169No Hit
AACTTAAGGAATTATAACAAAGAAGTAACACGTNN315090.14699006938841888No Hit
GACTGTGAATCATCAGAATGCTGAATGTAAACNNN309280.1442796936127779No Hit
AGTGTTGCGAAGCTAAGTCTGTTGGATNNNNNNNN308440.14388783205485392No Hit
AATAGATCCGACTTAGATAATGAAATTCCTANNNN307620.14350530053402333No Hit
CTTTATTGTAGATTTGTGTGGTGTTAATCCCCACTTTGCATACAATACCG290360.13545347852239456No Hit
TTTACGATCTGTTGAGGCTTATCCCTAGNNNNNNN289900.13523888766924572No Hit
AGAACTGAAACGGACAAGGGGAATCCGACNNNNNN288220.13445516455339773No Hit
GTTTTACGATCTGTTGAGGCTTATCCCTAGTGGTAAAGTGTTTT285870.13335888519491987No Hit
ACTTAAGGAATTATAACAAAGAAGTAACACGTNNN283670.13233258111464274No Hit
AATAGATCCGACTTAGATAATGAAATTCCTNNNNN280480.1308444401982409No Hit
TTTATTGTAGATTTGTGGTGTTTAATTCGACGGAATTGTCGTTGAATTAT269320.1256382795001078No Hit
TTACGATCTGTTGAGGCTTATCCCTAGTNNNNNNN268050.1250458221446751No Hit
TTGTTTTACGATCTGTTGAGGCTTATCCCTAGTNN267300.12469594575367153No Hit
AAAATTAAAAGATCTTGAAGCTGTATGCCAACCNN262770.12258269235200997No Hit
AATGAATTACTGTAGTTTACTTAAAGACGTNNNNN255530.11920521892418888No Hit
TTTATTGTAGATTTGTGGTGTTTAATNNNNNNNNN254680.11880869234771818No Hit
AACAGATCTATTCGGAAGAGGTATCGATNNNNNNN252980.11801563919477676No Hit
TTTACGATCTGTTGAGGCTTATCCCTNNNNNNNNN252900.11797831904640305No Hit
ATCCCCACTTTGCATACAATACCGGTAAGCANNNN248300.11583241051491448No Hit
AACTGGTCTGTAAAAGCAAAAAGAAGAAACNNNNN241550.11268352299588237No Hit
ATTAACGGTGCCTTCTCAGAACTCTTGTCTNNNNN238900.11144729308100311No Hit
TTTTACGATCTGTTGAGGCTTATCCCTAGNNNNNN228600.10664232397788745No Hit
ACGCGTGCTACACTGATATATATAACGAGTTATTTTTTGTTTTGTAGCAA225050.10498624239380389No Hit
AGGTAGTGGTAAGACGTGTATAGCTAGAGCTNNNN224750.10484629183740246No Hit
ACTTAAGGAATTATAACAAAGAAGTAACACGTANN222060.10359140184833633No Hit
CTTTATTGTAGATTTGTGGTGTTNNNNNNNNNNNN220270.10275636352847448No Hit
GAAAAGGATTGGCTCTGAGGACATTNNNNNNNNNN216030.10077839566466765No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)