FastQCFastQC Report
Fri 29 Sept 2023
SRR12538976_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12538976_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24305451
Total Bases902.6 Mbp
Sequences flagged as poor quality0
Sequence length35-151
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN277426311.414159729025394No Hit
TTTTACGATCTGTTGAGGCTTATCCCTAGTGGTAN12021674.94607979090781No Hit
TTTACGATCTGTTGAGGCTTATCCCTAGTGGTANN10964964.511317234969225No Hit
AAGGACTGGAGAGCAGGTAGATGTGCATTATCCNN4567981.8794055703800765No Hit
CTTAAGGAATTATAACAAAGAAGTAACACGTANNN3276941.3482325425683317No Hit
TTTTACGATCTGTTGAGGCTTATCCCTAGTNNNNN2951921.214509453044093No Hit
AATCCCCACTTTGCATACAATACCGGTAAGCAANN2801581.152655015535404No Hit
TTTTACGATCTGTTGAGGCTTATCCCTAGTGGTAAAGTGTTT2274520.9358065398580754No Hit
TTTACGATCTGTTGAGGCTTATCCCTAGTNNNNNN2216690.9120135232216016No Hit
CTTAAGGAATTATAACAAAGAAGTAACACGTNNNN2068470.8510313180364356No Hit
ACTAAAGATTGCCGTGTAGTAAGTATGTTNNNNNN1853440.7625614517500622No Hit
AGAAAAAAGTAGGTAAGGGAAGTCGGCNNNNNNNN1679700.6910795442553196No Hit
AGGACTGGAGAGCAGGTAGATGTGCATTATCCNNN1615430.6646369162209744No Hit
TTTACGATCTGTTGAGGCTTATCCCTAGTGGTNNN1543760.6351497036611252No Hit
TTTTACGATCTGTTGAGGCTTATCCCTAGTGGTNN1521700.6260735503323925No Hit
AATTGTGCTCCAGGAACTTTGATGGCTTCTCNNNN1191150.4900752510208512No Hit
AAAAGATCTTGAAGCTGTATGCCAACCNNNNNNNN1189040.48920713300074126No Hit
ATTAGGCTGTGCGACTGGATCTGTTATGTCNNNNN1102840.4537418375820305No Hit
TTTACGATCTGTTGAGGCTTATCCCTAGTGGTAAAGTGTTT1070470.44042383743465613No Hit
TTACGATCTGTTGAGGCTTATCCCTAGTGGTANNN1025410.4218847862563834No Hit
ATTAGGCTGTGCGACTGGATCTGTTATGTCTCNNN969350.3988200013239828No Hit
TTTTACGATCTGTTGAGGCTTATCCCTANNNNNNN952060.3917063707231765No Hit
AACGGTGCCTTCTCAGAACTCTTGTCTTCACANNN913040.3756523588062612No Hit
CCAAATCGTCTAGTGGTTAGGACTCCACGCTNNNN898130.3695179324177116No Hit
GTTTTACGATCTGTTGAGGCTTATCCCTAGTNNNN871830.3586973144419332No Hit
AAAAGATCTTGAAGCTGTATGCCAACCANNNNNNN824670.33929425954696335No Hit
ATCCCCACTTTGCATACAATACCGGTAAGCAANNN791150.325503114507112No Hit
AAGAAGATTAAGAACACAATGTGAAAGAGCNNNNN788010.32421122323547913No Hit
ATCTTTGGATAAGTATTTGTTAGGCCTTNNNNNNN728500.2997270036256476No Hit
ATCAGACTACATTTGGAATTCTACTTTTATGTNNN685970.28222887121082424No Hit
AATAGATCCGACTTAGATAATGAAATTCCTANNNN669700.27553489955812793No Hit
TTTTACGATCTGTTGAGGCTTATCCCTNNNNNNNN634180.2609208938357079No Hit
ATGCTTCCGATCCAGAATACCGTAAACACTNNNNN577940.23778205144187617No Hit
ACTCTAGTCTGGCTTTGTGAAACGACTNNNNNNNN539780.2220818696184654No Hit
AACGATTGGTGCCGACTTTTTAACTAAAGAANNNN529330.21778242255204397No Hit
GTTTTACGATCTGTTGAGGCTTATCCCTAGTGGTAAAGTGTTT483530.19893891292122085No Hit
AACAGATCTATTCGGAAGAGGTATCGATNNNNNNN478040.19668016034756977No Hit
TCCAAATCGTCTAGTGGTTAGGACTCCACGCTNNN476680.19612061508342304No Hit
TTTACGATCTGTTGAGGCTTATCCCTANNNNNNNN451240.18565382720114923No Hit
AGATTGTGCTGACTACGTCCCTGCCCTTTGTTTAAGGAATTATAACAAAG446240.1835966754947275No Hit
ATGGGATGTAGGAGGACAAGATANNNNNNNNNNNN443750.18257221394492948No Hit
AACGATTGGTGCCGACTTTTTAACTAAAGAAANNN437340.1799349454572968No Hit
TTAAGGAATTATAACAAAGAAGTAACACGTNNNNN437230.1798896881197555No Hit
TTAAGGAATTATAACAAAGAAGTAACACGTANNNN431210.17741287746522375No Hit
AACTTAAGGAATTATAACAAAGAAGTAACACGTNN429240.1766023596928936No Hit
GACTGTGAATCATCAGAATGCTGAATGTAAACNNN412760.16982198766852752No Hit
ATTAACGGTGCCTTCTCAGAACTCTTGTCTNNNNN402440.16557602654647305No Hit
AACTGGTCTGTAAAAGCAAAAAGAAGAAACNNNNN391750.16117783619814335No Hit
GACGTGGCATCTTTTCGGGCTAGTGAAGATTTNNN381520.1569689038068045No Hit
GTTTTACGATCTGTTGAGGCTTATCCCTAGTGGTA376620.1549528951345112No Hit
GTTTTACGATCTGTTGAGGCTTATCCCTANNNNNN372430.15322900200452977No Hit
AATCCCCACTTTGCATACAATACCGGTAAGCANNN355010.14606188545935642No Hit
AACGGTGCCTTCTCAGAACTCTTGTCTTCANNNNN354290.1457656556136317No Hit
AATAGATCCGACTTAGATAATGAAATTCCTNNNNN350640.14426393486794384No Hit
AAAATTAAAAGATCTTGAAGCTGTATGCCAACCNN333520.1372202474251558No Hit
AATGAATTACTGTAGTTTACTTAAAGACGTNNNNN323500.13309771540548662No Hit
TTTACGATCTGTTGAGGCTTATCCCTAGNNNNNNN323430.1330689152815967No Hit
GAAAGATATGATGAATTGTTTGGACAAGANNNNNN316490.13021358871308333No Hit
GTATGGGATGTAGGAGGACAAGATANNNNNNNNNN313620.12903278363359724No Hit
TTTACGATCTGTTGAGGCTTATCCCTNNNNNNNNN308380.12687688864526728No Hit
AGATTGTGCTGACTACGTCCCTGCCCTTTGTCTTAAGGAATTATAACAAA306720.12619391427873525No Hit
TGTTTTACGATCTGTTGAGGCTTATCCCTAGTNNN304030.12508716666068037No Hit
TTACGATCTGTTGAGGCTTATCCCTAGTNNNNNNN298960.12300121483036872No Hit
AGAACTGAAACGGACAAGGGGAATCCGACNNNNNN289150.11896508318236926No Hit
TTGTTTTACGATCTGTTGAGGCTTATCCCTAGTNN285260.11736461915477314No Hit
ACGATCTGTTGAGGCTTATCCCTAGTGGTNNNNNN275030.11315568676343425No Hit
TTTTACGATCTGTTGAGGCTTATCCCTAGNNNNNN266560.10967087177275582No Hit
AAACGATGTCGACTTGGAGGTTGTGCCCTTGAGGCGTGGCTTCCGGAGCT265250.10913189802567334No Hit
AAAATGATAGTAAAGATTTAGGACATTGGGCTNNN262020.10780297802332489No Hit
TTTTACGATCTGTTGAGGCTTATCCCTAGTGGTAAAGTGTTTT258510.10635885752541684No Hit
TTACGATCTGTTGAGGCTTATCCCTAGTGGTNNNN256260.10543313925752704No Hit
AACGGTGCCTTCTCAGAACTCTTGTCTTCNNNNNN247150.10168500884842663No Hit
GTTTTACGATCTGTTGAGGCTTATCCCTNNNNNNN246240.10131060723785788No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)