FastQCFastQC Report
Fri 29 Sept 2023
SRR12538977_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12538977_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17636310
Total Bases678.5 Mbp
Sequences flagged as poor quality0
Sequence length35-151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12128946.877254935981507No Hit
TTTTACGATCTGTTGAGGCTTATCCCTAGTGGTAAAGTGTTT10692406.062719469095293No Hit
TTTACGATCTGTTGAGGCTTATCCCTAGTGGTAAAGTGTTT4920322.7898806496370274No Hit
TTTACGATCTGTTGAGGCTTATCCCTAGTGGTANN4347532.4651018268560714No Hit
AGAAAAAAGTAGGTAAGGGAAGTCGGCNNNNNNNN3940842.234503702872086No Hit
ACTAAAGATTGCCGTGTAGTAAGTATGTTNNNNNN3746492.1243049141231927No Hit
TTTTACGATCTGTTGAGGCTTATCCCTAGTGGTAN3233661.8335241328826721No Hit
ACTCTAGTCTGGCTTTGTGAAACGACTNNNNNNNN2967981.682880375770215No Hit
AATCCCCACTTTGCATACAATACCGGTAAGCAANN2926841.6595535007039455No Hit
GTTTTACGATCTGTTGAGGCTTATCCCTAGTGGTAAAGTGTTT2849631.615774501582247No Hit
TTGTTTTACGATCTGTTGAGGCTTATCCCTAGTGGTAAAGTGTTT1973171.1188111345287082No Hit
CTTAAGGAATTATAACAAAGAAGTAACACGTANNN1682800.9541678503042871No Hit
TTTTACGATCTGTTGAGGCTTATCCCTAGTNNNNN1502000.8516520746119794No Hit
TTTTACGATCTGTTGAGGCTTATCCCTAGTGGTAAAGTGTTTT1456100.8256262222653151No Hit
TTTACGATCTGTTGAGGCTTATCCCTAGTNNNNNN1345960.7631755168739947No Hit
AGATTGTGCTGACTACGTCCCTGCCCTTTGTTTAAGGAATTATAACAAAG1204800.6831360981974121No Hit
ATCCCCACTTTGCATACAATACCGGTAAGCAANNN1129830.6406272060311936No Hit
TTTACGATCTGTTGAGGCTTATCCCTAGTGGTNNN1058200.6000121340575211No Hit
ATCAGATTGTGCTGACTACGTCCCTGCCCTTNNNN1030580.5843512616868268No Hit
TGTTTTACGATCTGTTGAGGCTTATCCCTAGTGGTAAAGTGTTT1020970.5789022760430045No Hit
AGATTGTGCTGACTACGTCCCTGCCCTTTGTCTTAAGGAATTATAACAAA988830.5606785092800024No Hit
AGCTTTTTGATCCTTCGATGTCGGCTCTTCCTNNN927010.5256258253568915No Hit
GTTTTACGATCTGTTGAGGCTTATCCCTAGTNNNN891760.5056386511690938No Hit
AGAACTGAAACGGACAAGGGGAATCCGACNNNNNN860010.4876360190992334No Hit
CTTAAGGAATTATAACAAAGAAGTAACACGTNNNN816280.46284058286569013No Hit
AGTGTTGCGAAGCTAAGTCTGTTGGATNNNNNNNN775820.43989927598233414No Hit
AATCACGTTTAGGGGCGAAAGACTNNNNNNNNNNN775220.43955906876211637No Hit
AGCTTTTTGATCCTTCGATGTCGGCTCTTCCNNNN767440.4351477151399584No Hit
ATCTTTGGATAAGTATTTGTTAGGCCTTNNNNNNN763360.43283430604247713No Hit
TTTTACGATCTGTTGAGGCTTATCCCTAGTGGTNN743900.42180025186674536No Hit
TTTACGATCTGTTGAGGCTTATCCCTAGTGGTAAAGTGTTTT645840.36619905184247725No Hit
AATTGTGCTCCAGGAACTTTGATGGCTTCTCNNNN637130.3612603770289817No Hit
TTGTTTTACGATCTGTTGAGGCTTATCCCTAGTNN634070.3595253202058707No Hit
TGTTTTACGATCTGTTGAGGCTTATCCCTAGTNNN613490.34785621255239896No Hit
AAGGACTGGAGAGCAGGTAGATGTGCATTATCCNN609560.3456278552599722No Hit
TTACGATCTGTTGAGGCTTATCCCTAGTGGTANNN593530.336538652359819No Hit
GACTGTGAATCATCAGAATGCTGAATGTAAACNNN579640.32866285521177613No Hit
AGGACCCGAGAGGCTTTGAACTAAGCGTGATGNNN539980.30617515795537725No Hit
AATCCCCACTTTGCATACAATACCGGTAAGCANNN529140.30002874751010844No Hit
ATTTCAATCACGTTTAGGGGCGAAAGACTNNNNNN489410.2775013594113508No Hit
ACGATCTGTTGAGGCTTATCCCTAGTGGTNNNNNN478150.2711168039119294No Hit
CTTTATTGTAGATTTGTGTGGTGTTNNNNNNNNNN474310.2689394777025353No Hit
TTTTTGATCCTTCGATGTCGGCTCTTCCTNNNNNN419900.23808835294911465No Hit
GTTTTACGATCTGTTGAGGCTTATCCCTAGTGGTAAAGTGTTTT418660.23738525802733113No Hit
TTTTACGATCTGTTGAGGCTTATCCCTANNNNNNN410360.23267905814765105No Hit
AAGACAAGAGTAGGATTGACAGATTNNNNNNNNNN407760.23120482686004046No Hit
TTTACGATCTGTTGAGGCTTATCCCTAGNNNNNNN365140.20703877398390025No Hit
AATGAATTACTGTAGTTTACTTAAAGACGTNNNNN364150.20647743207054087No Hit
TTACGATCTGTTGAGGCTTATCCCTAGTGGTNNNN354120.20079030137256604No Hit
TTACGATCTGTTGAGGCTTATCCCTAGTNNNNNNN347840.19722946580095269No Hit
TTTTGATCCTTCGATGTCGGCTCTTCCTNNNNNNN344340.19524492368301533No Hit
GTTTTACGATCTGTTGAGGCTTATCCCTANNNNNN338790.19209800689600037No Hit
ATCCCCACTTTGCATACAATACCGGTAAGCANNNN334460.18964284479009497No Hit
ATTAGGCTGTGCGACTGGATCTGTTATGTCTCNNN329080.1865923200488084No Hit
ATGCTTCCGATCCAGAATACCGTAAACACTNNNNN322690.18296911315348846No Hit
AAAAGATCTTGAAGCTGTATGCCAACCNNNNNNNN310470.17604022610171857No Hit
ATTAGGCTGTGCGACTGGATCTGTTATGTCNNNNN308120.17470774782253204No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG303300.17197474982011543No Hit
TGACTGTGAATCATCAGAATGCTGAATGTAAACNN301600.17101082936283155No Hit
TGTTTTACGATCTGTTGAGGCTTATCCCTAGNNNN301010.1706762922629507No Hit
AAGAAGATTAAGAACACAATGTGAAAGAGCNNNNN297700.16879948243141563No Hit
TTGTTTTACGATCTGTTGAGGCTTATCCCTAGTGGTAAAGTGTTTT295820.16773349980806643No Hit
TTTTTGATCCTTCGATGTCGGCTCTTCCNNNNNNN289080.16391183870095274No Hit
ACGCGTGCTACACTGATATATATAACGAGTTATTTTTTGTTTTGTAGCAA283590.16079894263595956No Hit
TTACGATCTGTTGAGGCTTATCCCTAGTGGTAAAGTGTTT283300.16063450914618763No Hit
TTTGATCCTTCGATGTCGGCTCTTCCTNNNNNNNN281780.15977265085496908No Hit
TTGTTTTACGATCTGTTGAGGCTTATCCCTAGNNN278110.15769171669130336No Hit
ATCAGACTACATTTGGAATTCTACTTTTATGTNNN270740.15351283800296092No Hit
TTGTTTTACGATCTGTTGAGGCTTATCCCTANNNN267900.1519025238272632No Hit
AGGACTGGAGAGCAGGTAGATGTGCATTATCCNNN266620.1511767484241318No Hit
AGTGTTGCGAAGCTAAGTCTGTTGGATAATGGCNN255910.14510404954324344No Hit
TTTACGATCTGTTGAGGCTTATCCCTANNNNNNNN251240.14245610334588132No Hit
TTTTACGATCTGTTGAGGCTTATCCCTAGNNNNNN248290.14078341784647697No Hit
AAAAGATCTTGAAGCTGTATGCCAACCANNNNNNN241590.13698443722071113No Hit
GTTTTACGATCTGTTGAGGCTTATCCCTAGTGGTN239180.13561793821950283No Hit
TTAAGGAATTATAACAAAGAAGTAACACGTANNNN232190.13165452410396505No Hit
TTTTACGATCTGTTGAGGCTTATCCCTNNNNNNNN230430.13065658292465943No Hit
ACTCTAGTCTGGCTTTGTGAAACGACTTNNNNNNN227270.12886482489817883No Hit
AACGATTGGTGCCGACTTTTTAACTAAAGAANNNN226480.12841688539155868No Hit
ATTAATGAATTACTGTAGTTTACTTAAAGACGTNN220650.12511120523510869No Hit
GTTTTACGATCTGTTGAGGCTTATCCCTAGNNNNN219950.12471429681152123No Hit
TGTTTTACGATCTGTTGAGGCTTATCCCTANNNNN219090.12422666646254234No Hit
AACTTAAGGAATTATAACAAAGAAGTAACACGTNN216830.12294521926638849No Hit
TTTTACGATCTGTTGAGGCTTATCCCTAGTGGTAAAGTGTTC212330.12039366511475474No Hit
GACGTGGCATCTTTTCGGGCTAGTGAAGATTTNNN209350.11870396925433947No Hit
AAAGATTGCCGTGTAGTAAGTATGTTNNNNNNNNN207840.11784778108345795No Hit
AATAGATCCGACTTAGATAATGAAATTCCTNNNNN203870.11559674330968327No Hit
AACGATTGGTGCCGACTTTTTAACTAAAGAAANNN196950.11167302003650423No Hit
AATAGATCCGACTTAGATAATGAAATTCCTANNNN196290.1112987920942646No Hit
AAAATTAAAAGATCTTGAAGCTGTATGCCAACCNN190330.10791940037343413No Hit
TTACGATCTGTTGAGGCTTATCCCTAGNNNNNNNN188420.10683640738907402No Hit
ACTAGTTTAAGACAAGAGTAGGATTGACAGATTNN187430.1062750654757146No Hit
GTTTTACGATCTGTTGAGGCTTATCCCTNNNNNNN185360.10510135056596306No Hit
TTTTGATCCTTCGATGTCGGCTCTTCCNNNNNNNN180830.10253278605331842No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)